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Mouse Anti-NFIB Recombinant Antibody (2D6) (CBMAB-N2222-WJ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
2D6
Antibody Isotype
IgG2a, κ
Application
ELISA, IHC, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
NFIB
Introduction
Recognizes and binds the palindromic sequence 5-TTGGCNNNNNGCCAA-3 present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Entrez Gene ID
4781
UniProt ID
O00712
Alternative Names
Nuclear Factor I B; CCAAT-Box-Binding Transcription Factor; TGGCA-Binding Protein; Nuclear Factor I/B; Nuclear Factor 1/B; NF-I/B; NF1-B; NFI-B;
Function
Transcriptional activator of GFAP, essential for proper brain development (PubMed:30388402).
Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Biological Process
Anterior commissure morphogenesisISS:UniProtKB
Brain developmentManual Assertion Based On ExperimentIMP:UniProtKB
Cell differentiation involved in salivary gland developmentIEA:Ensembl
Chondrocyte differentiationISS:UniProtKB
Club cell differentiationISS:UniProtKB
Commissural neuron axon guidanceISS:UniProtKB
DNA replicationIEA:UniProtKB-KW
Glial cell differentiationISS:UniProtKB
Hindbrain developmentIEA:Ensembl
Lung ciliated cell differentiationISS:UniProtKB
Negative regulation of DNA bindingIDA:UniProtKB
Negative regulation of epithelial cell proliferation involved in lung morphogenesisISS:UniProtKB
Negative regulation of mesenchymal cell proliferation involved in lung developmentISS:UniProtKB
Negative regulation of miRNA transcriptionManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of transcription by RNA polymerase IIIDA:UniProtKB
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Principal sensory nucleus of trigeminal nerve developmentISS:UniProtKB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Response to bacteriumIEA:Ensembl
Salivary gland cavitationIEA:Ensembl
Type I pneumocyte differentiationISS:UniProtKB
Type II pneumocyte differentiationISS:UniProtKB
Cellular Location
Nucleus
Involvement in disease
Macrocephaly, acquired, with impaired intellectual development (MACID):
An autosomal dominant disorder characterized by postnatal macrocephaly and borderline to mild mental retardation. Additional variable neurodevelopmental features include muscular hypotonia, motor and speech delay, attention deficit disorder, autism spectrum disorder, and behavioral abnormalities. Some patients present corpus callosum dysgenesis.
More Infomation

Campbell, C. E., Webber, K., Bard, J. E., Chaves, L. D., Osinski, J. M., & Gronostajski, R. M. (2024). Nuclear Factor IA (Nfia) and Nuclear Factor IB (Nfib) are jointly required for mouse postnatal neural stem cell self-renewal. Stem Cells and Development, (ja).

Zhang, W., Wang, Y., Liu, Y., Liu, C., Wang, Y., He, L., ... & Shang, Y. (2023). NFIB facilitates replication licensing by acting as a genome organizer. Nature Communications, 14(1), 5076.

Marinella, G., Conti, E., Buchignani, B., Sgherri, G., Pasquariello, R., Giordano, F., ... & Battaglia, A. (2023). Further characterization of NFIB‐associated phenotypes: Report of two new individuals. American Journal of Medical Genetics Part A, 191(2), 540-545.

Huang, H., Jin, J., Wu, L., Wu, H., Pi, H., Dong, Y., & Xiang, R. (2022). A de novo non-sense nuclear factor IB mutation (p. Tyr290*) is responsible for brain malformation and lung lobulation defects. Frontiers in Pediatrics, 10, 865181.

Sun, H., Li, N., Tan, J., Li, H., Zhang, J., Qu, L., & Lamont, S. J. (2022). Transcriptional regulation of RIP2 gene by NFIB is associated with cellular immune and inflammatory response to APEC infection. International journal of molecular sciences, 23(7), 3814.

Yu, J. G., Song, Y., Yang, A., Zhang, X., & Li, L. (2021). Serum nuclear factor IB as a novel and noninvasive indicator in the diagnosis of secondary hyperparathyroidism. Journal of Clinical Laboratory Analysis, 35(6), e23787.

Pan, D., Qian, B., Zhao, D., & Yao, B. (2021). Nfib promotes chondrocyte proliferation and inhibits differentiation by mildly regulating Sox9 and its downstream genes. Molecular Biology Reports, 48, 7487-7497.

Dasgupta, S., Ewing‑Graham, P. C., Van Den Bosch, T. P., Swagemakers, S., Santegoets, L. A., Van Doorn, H. C., ... & Van Kemenade, F. J. (2021). Nuclear factor IB is downregulated in vulvar squamous cell carcinoma (VSCC): Unravelling differentially expressed genes in VSCC through gene expression dataset analysis. Oncology Letters, 21(5), 1-11.

Barrus, K., Rego, S., Yip, T., Martin, P. M., Glen, O. A., Van Ziffle, J., & Slavotinek, A. M. (2020). The expanding spectrum of NFIB‐associated phenotypes in a diverse patient population—A report of two new patients. American Journal of Medical Genetics Part A, 182(12), 2959-2963.

Jain, S., Burchett, R., Monckton, E. A., & Godbout, R. (2019). A positive feedback loop involving nuclear factor IB and calpain 1 suppresses glioblastoma cell migration. Journal of Biological Chemistry, 294(34), 12638-12654.

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For research use only. Not intended for any clinical use.

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