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Mouse Anti-NFATC2 Antibody (G1-G9) (CBMAB-1454CQ)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
G1-G9
Antibody Isotype
IgG2a, κ
Application
IF, WB

Basic Information

Immunogen
Recaombinant GST/NFATC2 fusion protein
Specificity
Human, Mouse
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
NFATC2
Introduction
This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Diseases associated with NFATC2 include Leukostasis and Fibrosarcoma Of Bone. Among its related pathways are RANK Signaling in Osteoclasts and Immune response NFAT in immune response.
Entrez Gene ID
Human4773
Mouse18019
UniProt ID
HumanQ13469
MouseQ60591
Alternative Names
NFAT1; NFATP
Function
Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway.
Biological Process
B cell receptor signaling pathway Source: UniProtKB
Calcineurin-NFAT signaling cascade Source: GO_Central
Cell migration Source: UniProtKB
Cellular response to DNA damage stimulus Source: UniProtKB
Myotube cell development Source: Ensembl
Negative regulation of vascular associated smooth muscle cell differentiation Source: BHF-UCL
Positive regulation of B cell proliferation Source: UniProtKB
Positive regulation of gene expression Source: Ensembl
Positive regulation of myoblast fusion Source: Ensembl
Positive regulation of transcription, DNA-templated Source: MGI
Regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Response to xenobiotic stimulus Source: UniProtKB
Cellular Location
Nucleus
Cytoplasm
Note: Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
PTM
In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.
Ubiquitinated in endothelial cells by RNF213 downstream of the non-canonical Wnt signaling pathway, leading to its degradation by the proteasome.
More Infomation

Zhu, L., Zhou, X., Gu, M., Kim, J., Li, Y., Ko, C. J., ... & Sun, S. C. (2022). Dapl1 controls NFATc2 activation to regulate CD8+ T cell exhaustion and responses in chronic infection and cancer. Nature cell biology, 24(7), 1165-1176.

Lin, D. C., Zheng, S. Y., Zhang, Z. G., Luo, J. H., Zhu, Z. L., Li, L., ... & Zhou, R. X. (2021). TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway. Cancer Letters, 519, 211-225.

Simonett, S. P., Shin, S., Herring, J. A., Bacher, R., Smith, L. A., Dong, C., ... & Attie, A. D. (2021). Identification of direct transcriptional targets of NFATC2 that promote β cell proliferation. The Journal of clinical investigation, 131(21).

Hung, Y. P., Fisch, A. S., Diaz‐Perez, J. A., Iafrate, A. J., Lennerz, J. K., Nardi, V., ... & Nielsen, G. P. (2021). Identification of EWSR1–NFATC2 fusion in simple bone cysts. Histopathology, 78(6), 849-856.

Pižem, J., Šekoranja, D., Zupan, A., Boštjancic, E., Matjašic, A., Mavcic, B., ... & Salapura, V. (2020). FUS-NFATC2 or EWSR1-NFATC2 fusions are present in a large proportion of simple bone cysts. The American Journal of Surgical Pathology, 44(12), 1623-1634.

Perret, R., Escuriol, J., Velasco, V., Mayeur, L., Soubeyran, I., Delfour, C., ... & Le Loarer, F. (2020). NFATc2-rearranged sarcomas: clinicopathologic, molecular, and cytogenetic study of 7 cases with evidence of AGGRECAN as a novel diagnostic marker. Modern Pathology, 33(10), 1930-1944.

Kim, C., Beilina, A., Smith, N., Li, Y., Kim, M., Kumaran, R., ... & Masliah, E. (2020). LRRK2 mediates microglial neurotoxicity via NFATc2 in rodent models of synucleinopathies. Science translational medicine, 12(565), eaay0399.

Rathod, S., Ramsey, M., Finkelman, F. D., & Fernandez, C. A. (2020). Genetic inhibition of NFATC2 attenuates asparaginase hypersensitivity in mice. Blood Advances, 4(18), 4406-4416.

Bode-Lesniewska, B., Fritz, C., Exner, G. U., Wagner, U., & Fuchs, B. (2019). EWSR1-NFATC2 and FUS-NFATC2 gene fusion-associated mesenchymal tumors: clinicopathologic correlation and literature review. Sarcoma, 2019.

Diaz-Perez, J. A., Nielsen, G. P., Antonescu, C., Taylor, M. S., Lozano-Calderon, S. A., & Rosenberg, A. E. (2019). EWSR1/FUS-NFATc2 rearranged round cell sarcoma: clinicopathological series of 4 cases and literature review. Human pathology, 90, 45-53.

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For research use only. Not intended for any clinical use.

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