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Mouse Anti-NFATC2 Recombinant Antibody (CBWJN-0605) (CBMAB-N2164-WJ)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBWJN-0605
Application
IP, IF

Basic Information

Specificity
Human, Mouse, Rat
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
NFATC2
Introduction
This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012]
Entrez Gene ID
Human4773
Mouse18019
Rat311658
UniProt ID
HumanQ13469
MouseQ60591
RatD4A0I8
Alternative Names
Nuclear Factor Of Activated T Cells 2; Nuclear Factor Of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2; Nuclear Factor Of Activated T-Cells 2; NFAT Pre-Existing Subunit; NF-ATc2; NFAT1; NFATP; Nuclear Factor Of Activated T-Cells, Preexisting Component;
Function
Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway.
Biological Process
B cell receptor signaling pathway Source: UniProtKB
Calcineurin-NFAT signaling cascade Source: GO_Central
Cell migration Source: UniProtKB
Cellular response to DNA damage stimulus Source: UniProtKB
Myotube cell development Source: Ensembl
Negative regulation of vascular associated smooth muscle cell differentiation Source: BHF-UCL
Positive regulation of B cell proliferation Source: UniProtKB
Positive regulation of gene expression Source: Ensembl
Positive regulation of myoblast fusion Source: Ensembl
Positive regulation of transcription, DNA-templated Source: MGI
Regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Response to xenobiotic stimulus Source: UniProtKB
Cellular Location
Nucleus
Cytoplasm
Note: Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
PTM
In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.
Ubiquitinated in endothelial cells by RNF213 downstream of the non-canonical Wnt signaling pathway, leading to its degradation by the proteasome.
More Infomation

Zhu, L., Zhou, X., Gu, M., Kim, J., Li, Y., Ko, C. J., ... & Sun, S. C. (2022). Dapl1 controls NFATc2 activation to regulate CD8+ T cell exhaustion and responses in chronic infection and cancer. Nature cell biology, 24(7), 1165-1176.

Lin, D. C., Zheng, S. Y., Zhang, Z. G., Luo, J. H., Zhu, Z. L., Li, L., ... & Zhou, R. X. (2021). TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway. Cancer Letters, 519, 211-225.

Simonett, S. P., Shin, S., Herring, J. A., Bacher, R., Smith, L. A., Dong, C., ... & Attie, A. D. (2021). Identification of direct transcriptional targets of NFATC2 that promote β cell proliferation. The Journal of clinical investigation, 131(21).

Hung, Y. P., Fisch, A. S., Diaz‐Perez, J. A., Iafrate, A. J., Lennerz, J. K., Nardi, V., ... & Nielsen, G. P. (2021). Identification of EWSR1–NFATC2 fusion in simple bone cysts. Histopathology, 78(6), 849-856.

Pižem, J., Šekoranja, D., Zupan, A., Boštjancic, E., Matjašic, A., Mavcic, B., ... & Salapura, V. (2020). FUS-NFATC2 or EWSR1-NFATC2 fusions are present in a large proportion of simple bone cysts. The American Journal of Surgical Pathology, 44(12), 1623-1634.

Perret, R., Escuriol, J., Velasco, V., Mayeur, L., Soubeyran, I., Delfour, C., ... & Le Loarer, F. (2020). NFATc2-rearranged sarcomas: clinicopathologic, molecular, and cytogenetic study of 7 cases with evidence of AGGRECAN as a novel diagnostic marker. Modern Pathology, 33(10), 1930-1944.

Kim, C., Beilina, A., Smith, N., Li, Y., Kim, M., Kumaran, R., ... & Masliah, E. (2020). LRRK2 mediates microglial neurotoxicity via NFATc2 in rodent models of synucleinopathies. Science translational medicine, 12(565), eaay0399.

Rathod, S., Ramsey, M., Finkelman, F. D., & Fernandez, C. A. (2020). Genetic inhibition of NFATC2 attenuates asparaginase hypersensitivity in mice. Blood Advances, 4(18), 4406-4416.

Bode-Lesniewska, B., Fritz, C., Exner, G. U., Wagner, U., & Fuchs, B. (2019). EWSR1-NFATC2 and FUS-NFATC2 gene fusion-associated mesenchymal tumors: clinicopathologic correlation and literature review. Sarcoma, 2019.

Diaz-Perez, J. A., Nielsen, G. P., Antonescu, C., Taylor, M. S., Lozano-Calderon, S. A., & Rosenberg, A. E. (2019). EWSR1/FUS-NFATc2 rearranged round cell sarcoma: clinicopathological series of 4 cases and literature review. Human pathology, 90, 45-53.

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For research use only. Not intended for any clinical use.

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