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Mouse Anti-NELFB Recombinant Antibody (CBCNC-671) (CBMAB-C4033-CN)


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Summary

Host Animal
Mouse
Specificity
Mouse, Human, Rabbit, Rat, Sheep
Clone
CBCNC-671
Antibody Isotype
IgG1
Application
ELISA, WB

Basic Information

Immunogen
Recombinant full length human COBRA1 expressed in E. coli
Specificity
Mouse, Human, Rabbit, Rat, Sheep
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
HEPES, pH 7.5, 0.01% BSA, 50% glycerol

Target

Full Name
Negative Elongation Factor Complex Member B
Introduction
NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]
Entrez Gene ID
Human25920
Mouse58202
Rat311796
Sheep101103711
UniProt ID
HumanQ8WX92
MouseQ8C4Y3
RatD4ACV0
SheepA0A6P7E113
Alternative Names
Negative Elongation Factor Complex Member B; Cofactor Of BRCA1; COBRA1; NELF-B; Negative Elongation Factor Protein B; Negative Elongation Factor B; KIAA1182;
Function
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed:12612062).

The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed:10199401).

May be able to induce chromatin unfolding (PubMed:11739404).

Essential for early embryogenesis; plays an important role in maintaining the undifferentiated state of embryonic stem cells (ESCs) by preventing unscheduled expression of developmental genes (By similarity).

Plays a key role in establishing the responsiveness of stem cells to developmental cues; facilitates plasticity and cell fate commitment in ESCs by establishing the appropriate expression level of signaling molecules (By similarity).

Supports the transcription of genes involved in energy metabolism in cardiomyocytes; facilitates the association of transcription initiation factors with the promoters of the metabolism-related genes (By similarity).

(Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II (PubMed:23884411).

In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1 (PubMed:23884411).
Biological Process
Cell population proliferation Source: UniProtKB
Negative regulation of stem cell differentiation Source: UniProtKB
Negative regulation of transcription elongation from RNA polymerase II promoter Source: UniProtKB
Stem cell differentiation Source: UniProtKB
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

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