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Mouse Anti-NCK1 Recombinant Antibody (10H10) (CBMAB-0771-CN)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
10H10
Antibody Isotype
IgG
Application
ELISA, MA

Basic Information

Immunogen
Recombinant protein of NCK1 (aa. 270-377)
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
NCK Adaptor Protein 1
Introduction
The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It's an adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. This protein plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2.
Entrez Gene ID
4690
UniProt ID
P16333
Alternative Names
NCK; nck-1; NCKalpha
Function
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors.
Biological Process
Actin filament organization Source: Ensembl
Cell migration Source: GO_Central
Ephrin receptor signaling pathway Source: Ensembl
Lamellipodium assembly Source: Ensembl
Negative regulation of cell death Source: UniProtKB
Negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation Source: ParkinsonsUK-UCL
Negative regulation of insulin receptor signaling pathway Source: FlyBase
Negative regulation of peptidyl-serine phosphorylation Source: ParkinsonsUK-UCL
Negative regulation of PERK-mediated unfolded protein response Source: ParkinsonsUK-UCL
Negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
Peptidyl-serine dephosphorylation Source: ParkinsonsUK-UCL
Positive regulation of actin filament polymerization Source: UniProtKB
Positive regulation of cap-dependent translational initiation Source: ParkinsonsUK-UCL
Positive regulation of cap-independent translational initiation Source: ParkinsonsUK-UCL
Positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
Positive regulation of neuron projection development Source: Ensembl
Positive regulation of T cell proliferation Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Positive regulation of translation in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
Regulation of cell migration Source: Ensembl
Response to other organism Source: Ensembl
Signal complex assembly Source: UniProtKB
Signal transduction Source: GO_Central
Substrate-dependent cell migration, cell extension Source: Ensembl
T cell activation Source: UniProtKB
Cellular Location
Nucleus
Cytoplasm
Endoplasmic reticulum
Note: Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.
PTM
Phosphorylated on Ser and Tyr residues. Phosphorylated in response to activation of EGFR and FcERI. Phosphorylated by activated PDGFRB.
More Infomation

Golding, A. P., Ferrier, B., New, L. A., Lu, P., Martin, C. E., Shata, E., ... & Jones, N. (2023). Distinct requirements for adaptor proteins NCK1 and NCK2 in mammary gland development. Journal of Mammary Gland Biology and Neoplasia, 28(1), 19.

Paensuwan, P., Ngoenkam, J., Wangteeraprasert, A., & Pongcharoen, S. (2022). Essential function of adaptor protein Nck1 in platelet-derived growth factor receptor signaling in human lens epithelial cells. Scientific Reports, 12(1), 1063.

Alfaidi, M., Scott, M. L., & Orr, A. W. (2021). Sinner or Saint?: Nck adaptor proteins in vascular biology. Frontiers in Cell and Developmental Biology, 9, 688388.

Li, B., Zhang, X., & Meng, X. (2020). NCK1-AS1 promotes NCK1 expression to facilitate tumorigenesis and chemo-resistance in ovarian cancer. Biochemical and biophysical research communications, 522(2), 292-299.

Wines-Samuelson, M., Chowdhury, S., & Berk, B. C. (2020). Nck1 is a critical adaptor between proatherogenic blood flow, inflammation, and atherosclerosis. The Journal of Clinical Investigation, 130(8), 3968-3970.

Alfaidi, M., Acosta, C. H., Wang, D., Traylor, J. G., & Orr, A. W. (2020). Selective role of Nck1 in atherogenic inflammation and plaque formation. The Journal of clinical investigation, 130(8), 4331-4347.

Alfaidi, M., Acosta, C. H., Lindquist, J. M., Cockerham, E. D., & Orr, A. W. (2019). The differential roles of the adaptor proteins Nck1 and Nck2 in shear stress-induced endothelial activation. bioRxiv, 668129.

Alfaidi, M., Bhattarai, U., Cockerham, E. D., & Orr, A. W. (2019). The Adaptor Protein Nck1, but not Nck2, Mediates Shear Stress-Induced Endothelial Permeability. bioRxiv, 651687.

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For research use only. Not intended for any clinical use.

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