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Mouse Anti-MYOC Recombinant Antibody (A1274) (CBMAB-AP12344LY)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
A1274
Antibody Isotype
IgG2a
Application
ELISA, WB

Basic Information

Immunogen
Fusion Protein of MYOC
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
Affinity purity
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Myocilin
Introduction
MYOC encodes the protein myocilin, which is believed to have a role in cytoskeletal function. MYOC is expressed in many occular tissues, including the trabecular meshwork, and was revealed to be the trabecular meshwork glucocorticoid-inducible response protein (TIGR). The trabecular meshwork is a specialized eye tissue essential in regulating intraocular pressure, and mutations in MYOC have been identified as the cause of hereditary juvenile-onset open-angle glaucoma. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human4653
Mouse17926
Rat81523
UniProt ID
HumanQ99972
MouseO70624
RatQ9R1J4
Alternative Names
Myocilin; Trabecular Meshwork Inducible Glucocorticoid Response Protein; Juvenile-Onset Open-Angle Glaucoma 1; Myocilin 55 KDa Subunit; GLC1A; TIGR; Myocilin Trabecular Meshwork Inducible Glucocorticoid Response Protein;
Function
Secreted glycoprotein regulating the activation of different signaling pathways in adjacent cells to control different processes including cell adhesion, cell-matrix adhesion, cytoskeleton organization and cell migration. Promotes substrate adhesion, spreading and formation of focal contacts. Negatively regulates cell-matrix adhesion and stress fiber assembly through Rho protein signal transduction. Modulates the organization of actin cytoskeleton by stimulating the formation of stress fibers through interactions with components of Wnt signaling pathways. Promotes cell migration through activation of PTK2 and the downstream phosphatidylinositol 3-kinase signaling. Plays a role in bone formation and promotes osteoblast differentiation in a dose-dependent manner through mitogen-activated protein kinase signaling. Mediates myelination in the peripheral nervous system through ERBB2/ERBB3 signaling. Plays a role as a regulator of muscle hypertrophy through the components of dystrophin-associated protein complex. Involved in positive regulation of mitochondrial depolarization. Plays a role in neurite outgrowth. May participate in the obstruction of fluid outflow in the trabecular meshwork.
Biological Process
Bone development Source: UniProtKB
Clustering of voltage-gated sodium channels Source: UniProtKB
ERBB2-ERBB3 signaling pathway Source: UniProtKB
Myelination in peripheral nervous system Source: UniProtKB
Negative regulation of cell-matrix adhesion Source: UniProtKB
Negative regulation of Rho protein signal transduction Source: UniProtKB
Negative regulation of stress fiber assembly Source: UniProtKB
Neuron projection development Source: UniProtKB
Non-canonical Wnt signaling pathway via JNK cascade Source: UniProtKB
Osteoblast differentiation Source: UniProtKB
Positive regulation of cell migration Source: UniProtKB
Positive regulation of focal adhesion assembly Source: UniProtKB
Positive regulation of mitochondrial depolarization Source: UniProtKB
Positive regulation of phosphatidylinositol 3-kinase signaling Source: UniProtKB
Positive regulation of protein kinase B signaling Source: UniProtKB
Positive regulation of stress fiber assembly Source: UniProtKB
Positive regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
Regulation of MAPK cascade Source: UniProtKB
Signal transduction Source: GO_Central
Skeletal muscle hypertrophy Source: UniProtKB
Cellular Location
Secreted
extracellular space
extracellular matrix
extracellular exosome
Golgi apparatus
Endoplasmic reticulum
Rough endoplasmic reticulum
Mitochondrion
Mitochondrion intermembrane space
Mitochondrion inner membrane
Mitochondrion outer membrane
Other locations
Cytoplasmic vesicle
Cell projection
cilium
Note: Located preferentially in the ciliary rootlet and basal body of the connecting cilium of photoreceptor cells, and in the rough endoplasmic reticulum (PubMed:9169133). It is only imported to mitochondria in the trabecular meshwork (PubMed:17516541). Localizes to the Golgi apparatus in Schlemm's canal endothelial cells (PubMed:11053284). Appears in the extracellular space of trabecular meshwork cells by an unconventional mechanism, likely associated with exosome-like vesicles (PubMed:15944158). Localizes in trabecular meshwork extracellular matrix (PubMed:15944158).
Myocilin, C-terminal fragment:
Secreted
Myocilin, N-terminal fragment:
Endoplasmic reticulum
Note: Remains retained in the endoplasmic reticulum.
Involvement in disease
Glaucoma 1, open angle, A (GLC1A):
A form of primary open angle glaucoma (POAG). POAG is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. However, glaucoma can occur at any intraocular pressure. The disease is generally asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place.
PTM
Different isoforms may arise by post-translational modifications.
Glycosylated.
Palmitoylated.
Undergoes a calcium-dependent proteolytic cleavage at Arg-226 by CAPN2 in the endoplasmic reticulum. The result is the production of two fragments, one of 35 kDa containing the C-terminal olfactomedin-like domain, and another of 20 kDa containing the N-terminal leucine zipper-like domain.
More Infomation

Saccuzzo, E. G., Youngblood, H. A., & Lieberman, R. L. (2023). Myocilin misfolding and glaucoma: A 20-year update. Progress in Retinal and Eye Research, 101188.

Zhou, B., Lin, X., Li, Z., Yao, Y., Yang, J., & Zhu, Y. (2022). Structure‒function‒pathogenicity analysis of C-terminal myocilin missense variants based on experiments and 3D models. Frontiers in Genetics, 13, 1019208.

Nakahara, E., & Hulleman, J. D. (2022). A simple secretion assay for assessing new and existing myocilin variants. Current eye research, 47(6), 918-922.

Sharma, R., & Grover, A. (2021). Myocilin-associated glaucoma: a historical perspective and recent research progress. Molecular Vision, 27, 480.

Atienzar-Aroca, R., Aroca-Aguilar, J. D., Alexandre-Moreno, S., Ferre-Fernández, J. J., Bonet-Fernández, J. M., Cabañero-Varela, M. J., & Escribano, J. (2021). Knockout of myoc Provides Evidence for the Role of Myocilin in Zebrafish Sex Determination Associated with Wnt Signalling Downregulation. Biology, 10(2), 98.

Judge, S. M., Deyhle, M. R., Neyroud, D., Nosacka, R. L., D'Lugos, A. C., Cameron, M. E., ... & Judge, A. R. (2020). MEF2c-dependent downregulation of myocilin mediates cancer-induced muscle wasting and associates with cachexia in patients with cancer. Cancer research, 80(9), 1861-1874.

O’Gorman, L., Cree, A. J., Ward, D., Griffiths, H. L., Sood, R., Denniston, A. K., ... & Gibson, J. (2019). Comprehensive sequencing of the myocilin gene in a selected cohort of severe primary open-angle glaucoma patients. Scientific reports, 9(1), 3100.

Alward, W. L., Van Der Heide, C., Khanna, C. L., Roos, B. R., Sivaprasad, S., Kam, J., ... & NEIGHBORHOOD Consortium. (2019). Myocilin mutations in patients with normal-tension glaucoma. JAMA ophthalmology, 137(5), 559-563.

Wang, H., Li, M., Zhang, Z., Xue, H., Chen, X., & Ji, Y. (2019). Physiological function of myocilin and its role in the pathogenesis of glaucoma in the trabecular meshwork. International journal of molecular medicine, 43(2), 671-681.

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For research use only. Not intended for any clinical use.

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