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Mouse Anti-MUC4 Recombinant Antibody (4F12) (CBMAB-0222-CN)


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Summary

Host Animal
Mouse
Specificity
Rat, Mouse, Human
Clone
4F12
Antibody Isotype
IgG1
Application
ELISA

Basic Information

Specificity
Rat, Mouse, Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
mucin 4, cell surface associated
Introduction
Mucin 4 (MUC 4) is a mucin protein that in humans is encoded by the MUC4 gene. Like other mucins, MUC-4 is a high-molecular weight glycoprotein. The protein may play a role in tumor progression. It also has the ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation.
Entrez Gene ID
Human4585
Mouse140474
Rat303887
UniProt ID
HumanQ99102
MouseQ8JZM8
RatQ63661
Alternative Names
Psmc; ASGP-1
Function
Membrane-bound mucin, a family of highly glycosylated proteins that constitute the major component of the mucus, the slimy and viscous secretion covering epithelial surfaces (PubMed:10880978).

These glycoproteins play important roles in the protection of the epithelium and are implicated in epithelial renewal and differentiation (PubMed:10880978).

Regulates cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and its ability to act as an intramembrane ligand for ERBB2. Plays an important role in proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. In polarized epithelial cells, segregates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. Its ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation.
Biological Process
Cell-matrix adhesion Source: InterPro
Maintenance of gastrointestinal epithelium Source: UniProtKB
Regulation of signaling receptor activity Source: GO_Central
Cellular Location
Chain Mucin-4 beta chain:
Plasma membrane
Cell membrane
Note: Isoforms lacking the Cys-rich region, EGF-like domains and transmembrane region are secreted. Secretion occurs by splicing or proteolytic processing.
Chain Mucin-4 alpha chain:
Plasma membrane
Cell membrane
Secreted
Note: Forms a complex with Mucin-4 beta chain at the cell membrane.
Isoform 3:
Plasma membrane
Cell membrane
Isoform 11:
Secreted
Isoform 15:
Secreted
Isoform 17:
Plasma membrane
Cell membrane
Topology
Helical: 5369-5389
PTM
Proteolytically cleaved into 2 chains, mucin-4 alpha chain and mucin-4 beta chain.
Mucin-4 alpha chain:
Highly O-glycosylated.
Mucin-4 beta chain:
Is predominantly N-glycosylated.
More Infomation

Martinez-Carrasco, R., Rachagani, S., Batra, S. K., Argüeso, P., & Fini, M. E. (2023). Roles unveiled for membrane-associated mucins at the ocular surface using a Muc4 knockout mouse model. Scientific reports, 13(1), 13558.

Arruda, B. L., Kanefsky, R. A., Hau, S., Janzen, G. M., Anderson, T. K., & Baker, A. L. V. (2023). Mucin 4 is a cellular biomarker of necrotizing bronchiolitis in influenza A virus infection. Microbes and Infection, 105169.

Dreyer, C. A., VanderVorst, K., Free, S., Rowson-Hodel, A., & Carraway, K. L. (2022). The role of membrane mucin MUC4 in breast cancer metastasis. Endocrine-related cancer, 29(1), R17-R32.

Kim, J. H., Park, H. S., Lee, J. Y., Ko, E. J., Kim, Y. R., Cho, H. Y., ... & Kim, N. K. (2022). Association study between Mucin 4 (MUC4) polymorphisms and idiopathic recurrent pregnancy loss in a Korean population. Genes, 13(6), 937.

Sagar, S., Leiphrakpam, P. D., Thomas, D., McAndrews, K. L., Caffrey, T. C., Swanson, B. J., ... & Radhakrishnan, P. (2021). MUC4 enhances gemcitabine resistance and malignant behaviour in pancreatic cancer cells expressing cancer-associated short O-glycans. Cancer letters, 503, 91-102.

Peng, L., Li, Y., Gu, H., Xiang, L., Xiong, Y., Wang, R., ... & Wang, J. (2021). Mucin 4 mutation is associated with tumor mutation burden and promotes antitumor immunity in colon cancer patients. Aging (Albany NY), 13(6), 9043.

Yu, J., Xu, L., Yan, J., Yu, J., Wu, X., Dai, J., ... & Kong, Y. (2020). MUC4 isoforms expression profiling and prognosis value in Chinese melanoma patients. Clinical and Experimental Medicine, 20, 299-311.

Gautam, S. K., Kumar, S., Dam, V., Ghersi, D., Jain, M., & Batra, S. K. (2020, February). MUCIN-4 (MUC4) is a novel tumor antigen in pancreatic cancer immunotherapy. In Seminars in immunology (Vol. 47, p. 101391). Academic Press.

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For research use only. Not intended for any clinical use.

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