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Mouse Anti-MKI67 Recombinant Antibody (8K5) (CBMAB-K2152-LY)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
8K5
Antibody Isotype
IgG
Application
IHC

Basic Information

Immunogen
Synthetic Peptide
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
50% glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/mL
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
marker of proliferation Ki-67
Introduction
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
Entrez Gene ID
Human4288
Mouse17345
Rat291234
UniProt ID
HumanP46013
MouseE9PVX6
RatD4A0Y6
Alternative Names
Marker Of Proliferation Ki-67; Antigen Identified By Monoclonal Antibody Ki-67; Protein Phosphatase 1, Regulatory Subunit 105; Antigen KI-67; Antigen Ki67; Proliferation-Related Ki-67 Antigen;
Function
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226).

Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226).

Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226).

Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551).

Does not contribute to the internal structure of mitotic chromosomes (By similarity).

May play a role in chromatin organization (PubMed:24867636).

It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Biological Process
Cell cycle Source: UniProtKB-KW
Cell population proliferation Source: ProtInc
Regulation of chromatin organization Source: UniProtKB
Regulation of chromosome segregation Source: UniProtKB
Regulation of mitotic nuclear division Source: UniProtKB
Cellular Location
Nucleus
nucleolus
Other locations
Chromosome
Note: Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).
PTM
Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.
More Infomation

Andrés-Sánchez, N., Fisher, D., & Krasinska, L. (2022). Physiological functions and roles in cancer of the proliferation marker Ki-67. Journal of Cell Science, 135(11), jcs258932.

Mrouj, K., Andrés-Sánchez, N., Dubra, G., Singh, P., Sobecki, M., Chahar, D., ... & Fisher, D. (2021). Ki-67 regulates global gene expression and promotes sequential stages of carcinogenesis. Proceedings of the National Academy of Sciences, 118(10), e2026507118.

Davey, M. G., Hynes, S. O., Kerin, M. J., Miller, N., & Lowery, A. J. (2021). Ki-67 as a prognostic biomarker in invasive breast cancer. Cancers, 13(17), 4455.

Uxa, S., Castillo-Binder, P., Kohler, R., Stangner, K., Müller, G. A., & Engeland, K. (2021). Ki-67 gene expression. Cell Death & Differentiation, 28(12), 3357-3370.

Li, Z., Li, F., Pan, C., He, Z., Pan, X., Zhu, Q., ... & Chen, L. (2021). Tumor cell proliferation (Ki-67) expression and its prognostic significance in histological subtypes of lung adenocarcinoma. Lung Cancer, 154, 69-75.

Li, C., Zheng, M., Zheng, X., Fang, X., Dong, J., Wang, C., & Wang, T. (2021). Predictive Ki-67 proliferation index of cervical squamous cell carcinoma based on IVIM-DWI combined with texture features. Contrast Media & Molecular Imaging, 2021.

Xiong, D. D., Zeng, C. M., Jiang, L., Luo, D. Z., & Chen, G. (2019). Ki-67/MKI67 as a predictive biomarker for clinical outcome in gastric cancer patients: an updated meta-analysis and systematic review involving 53 studies and 7078 patients. Journal of Cancer, 10(22), 5339.

Menon, S. S., Guruvayoorappan, C., Sakthivel, K. M., & Rasmi, R. R. (2019). Ki-67 protein as a tumour proliferation marker. Clinica chimica acta, 491, 39-45.

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For research use only. Not intended for any clinical use.

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