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Mouse Anti-MICA (AA 23-308) Recombinant Antibody (CBFYM-2204) (CBMAB-M2387-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-2204
Antibody Isotype
IgG2
Application
WB, Neut

Basic Information

Specificity
Human
Antibody Isotype
IgG2
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Concentration
0.5 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 23-308

Target

Full Name
MHC Class I Polypeptide-Related Sequence A
Introduction
This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants.
Entrez Gene ID
100507436
UniProt ID
Q29983
Alternative Names
MHC Class I Polypeptide-Related Sequence A; PERB11.1; MIC-A; Truncated MHC Class I Polypeptide-Related Sequence A; MHC Class I Chain-Related Protein A; Stress Inducible Class I Homolog; MHC Class I Related Sequence A; MHC Class I Related Chain A; HLA Class I Antigen
Function
Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Biological Process
Cellular response to DNA damage stimulus Source: UniProtKB
Cytolysis Source: UniProtKB-KW
Defense response to bacterium Source: UniProtKB
Defense response to virus Source: UniProtKB
Gamma-delta T cell activation Source: UniProtKB
Immune response to tumor cell Source: UniProtKB
Natural killer cell mediated cytotoxicity Source: UniProtKB
Negative regulation of natural killer cell activation Source: CACAO
Negative regulation of natural killer cell mediated cytotoxicity Source: CACAO
Response to heat Source: UniProtKB
T cell mediated cytotoxicity Source: UniProtKB
Cellular Location
Cytoplasm
Plasma membrane
Cell membrane
Note: Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum.
Involvement in disease
Anti-MICA antibodies and ligand shedding are involved in the progression of monoclonal gammopathy of undetermined significance (MGUS)to multiple myeloma.
Psoriasis 1 (PSORS1):
A common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
Psoriatic arthritis (PSORAS):
An inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoid like pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
Topology
Extracellular: 24-307
Helical: 308-328
Cytoplasmic: 329-383
PTM
N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation.
Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation.
More Infomation

Ren, X., Mao, A., Tan, S., Liu, J., & Wei, X. (2022). Analysis of the association between MICA gene polymorphisms and schizophrenia. Journal of Clinical Laboratory Analysis, 36(11), e24721.

Espinoza, I., Agarwal, S., Sakiyama, M., Shenoy, V., Orr, W. S., Al Diffalha, S., ... & Gomez, C. R. (2021). Expression of MHC class I polypeptide-related sequence A (MICA) in colorectal cancer.

Closa, L., Vidal, F., Herrero, M. J., & Caro, J. L. (2021). High‐throughput genotyping of HLA‐G, HLA‐F, MICA, and MICB and analysis of frequency distributions in healthy blood donors from Catalonia. HLA, 97(5), 420-427.

Otoyama, Y., Arai, J., Goto, K., Nozawa, H., Nakagawa, R., Muroyama, R., ... & Yoshida, H. (2021). Retinoids decrease soluble MICA concentration by inhibiting the enzymatic activity of ADAM9 and ADAM10. Anticancer Research, 41(5), 2307-2320.

Gong, X., Chen, P., Ma, P., Gao, J., Yang, J., Guo, H., ... & He, Y. (2020). MICA polymorphisms associated with antithyroid drug‐induced agranulocytosis in the Chinese Han population. Immunity, Inflammation and Disease, 8(4), 695-703.

Plasil, M., Wijkmark, S., Elbers, J. P., Oppelt, J., Burger, P. A., & Horin, P. (2019). The major histocompatibility complex of Old World camelids: class I and class I‐related genes. Hla, 93(4), 203-215.

Sakiyama, M. J., Espinoza, I., Reddy, A., de Carlo, F., Kumar, A., Levenson, A. S., ... & Gomez, C. R. (2019). Race-associated expression of MHC class I polypeptide-related sequence A (MICA) in prostate cancer. Experimental and molecular pathology, 108, 173-182.

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For research use only. Not intended for any clinical use.

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