Mouse Anti-MCAM Recombinant Antibody (A1228) (CBMAB-AP4900LY)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

A1228

Antibody Isotype

IgG1, κ

Application

FC, IF, IHC

Basic Information

Immunogen

Recombinant human MUC18 protein

Specificity

Human

Antibody Isotype

IgG1, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

Affinity purity

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Melanoma Cell Adhesion Molecule

Entrez Gene ID

4162

UniProt ID

P43121

Alternative Names

Cell Surface Glycoprotein MUC18, Cell Surface Glycoprotein P1H12, Gicerin, Melanoma Adhesion Molecule (MCAM), Melanoma Associated Glycoprotein MUC18, Melanoma Cell Adhesion Molecule, Melanoma-associated Antigen A32, Mel-CAM, S-endo 1 Endothelial-associated Antigen, Sendo1

Function

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

Biological Process

Anatomical structure morphogenesis Source: ProtInc
Angiogenesis Source: MGI
Cell adhesion Source: ProtInc
Glomerular filtration Source: UniProtKB
Positive regulation of cell migration Source: Ensembl
Vascular wound healing Source: UniProtKB

Cellular Location

Membrane

Topology

Extracellular: 24-559
Helical: 560-583
Cytoplasmic: 584-646

More Infomation

References

Pang, Y., Maxwell, E., Sindrewicz-Goral, P., Shapanis, A., Li, S., Morgan, M., & Yu, L. G. (2022). Galectin-3 Is a Natural Binding Ligand of MCAM (CD146, MUC18) in Melanoma Cells and Their Interaction Promotes Melanoma Progression. Biomolecules, 12(10), 1451.

Ikeguchi, R., Shimizu, Y., Kondo, A., Kanda, N., So, H., Kojima, H., & Kitagawa, K. (2021). Melanoma cell adhesion molecule expressing helper T cells in CNS inflammatory demyelinating diseases. Neurology-Neuroimmunology Neuroinflammation, 8(6).

Wang, Z., Xu, Q., Zhang, N., Du, X., Xu, G., & Yan, X. (2020). CD146, from a melanoma cell adhesion molecule to a signaling receptor. Signal transduction and targeted therapy, 5(1), 148.

Petersen, E. R., Ammitzbøll, C., Søndergaard, H. B., Oturai, A. B., Sørensen, P. S., Nilsson, A. C., ... & Sellebjerg, F. (2019). Expression of melanoma cell adhesion molecule-1 (MCAM-1) in natalizumab-treated multiple sclerosis. Journal of Neuroimmunology, 337, 577085.

Chen, Y., Sumardika, I. W., Tomonobu, N., Ruma, I. M. W., Kinoshita, R., Kondo, E., ... & Sakaguchi, M. (2019). Melanoma cell adhesion molecule is the driving force behind the dissemination of melanoma upon S100A8/A9 binding in the original skin lesion. Cancer Letters, 452, 178-190.

Čorić, M. (2019). Development and characterisation of monoclonal antibodies to tumour endothelial marker ‘melanoma cell adhesion molecule’(MCAM) and approaches to targeting MCAM on tumour vessels (Doctoral dissertation, University of Birmingham).

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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