Rabbit Anti-MBNL3 Recombinant Antibody (CBFYM-1838) (CBMAB-M2006-FY)

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Datasheet

Summary

Host Animal

Rabbit

Specificity

Human

Clone

CBFYM-1838

Antibody Isotype

IgG

Application

WB, IF

Basic Information

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

MBNL3

Introduction

This gene encodes a member of the muscleblind-like family of proteins. The encoded protein may function in regulation of alternative splicing and may play a role in the pathophysiology of myotonic dystrophy. Alternatively spliced transcript variants have been described.

Entrez Gene ID

55796

UniProt ID

Q9NUK0

Alternative Names

Muscleblind Like Splicing Regulator 3; Muscleblind-Like X-Linked Protein; Cys3His CCG1-Required Protein; MBLX39; CHCR; MBXL; Muscleblind-Like Splicing Regulator 3

Function

Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. May play a role in myotonic dystrophy pathophysiology (DM). Could inhibit terminal muscle differentiation, acting at approximately the time of myogenin induction.

Biological Process

mRNA processing Source: UniProtKB-KW
Negative regulation of myoblast differentiation Source: Ensembl
Regulation of alternative mRNA splicing, via spliceosome Source: GO_Central
Regulation of RNA splicing Source: UniProtKB
RNA splicing Source: UniProtKB-KW

Cellular Location

Cytoplasm
Nucleus
Note: Greater concentration in the nucleus. Expressed in or near large cytoplasmic adhesion plaques (PubMed:16273094). Location in the cytoplasm is microtubule-dependent (PubMed:16273094). In both DM1 and DM2 patients, colocalizes with nuclear foci of retained expanded-repeat transcripts (PubMed:11929853).

More Infomation

References

Spruce, T., Plass, M., Gohr, A., Ray, D., Martínez de Lagrán, M., Rot, G., ... & Irimia, M. (2022). The X-linked splicing regulator MBNL3 has been co-opted to restrict placental growth in eutherians. Plos Biology, 20(4), e3001615.

Zhou, J., Liu, T., Xu, H., Wang, Y., Yang, T., & Liu, L. (2022). LncRNA FIRRE promotes the proliferation and metastasis of hepatocellular carcinoma by regulating the expression of PXN through interacting with MBNL3. Biochemical and Biophysical Research Communications, 625, 188-195.

Sun, X., Diao, X., Zhu, X., Yin, X., & Cheng, G. (2021). Nanog-mediated stem cell properties are critical for MBNL3-associated paclitaxel resistance of ovarian cancer. The journal of biochemistry, 169(6), 747-756.

Oladimeji, P. O., Bakke, J., Wright, W. C., & Chen, T. (2020). KANSL2 and MBNL3 are regulators of pancreatic ductal adenocarcinoma invasion. Scientific reports, 10(1), 1485.

Yu, Z., Wang, G., Zhang, C., Liu, Y., Chen, W., Wang, H., & Liu, H. (2020). LncRNA SBF2-AS1 affects the radiosensitivity of non-small cell lung cancer via modulating microRNA-302a/MBNL3 axis. Cell cycle, 19(3), 300-316.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-MBNL3 Antibody (5A11)

Mouse Anti-MBNL3 Antibody (1D11)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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SDS
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