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Rabbit Anti-MBD2 Recombinant Antibody (CBFYM-1800) (CBMAB-M1968-FY)


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Summary

Host Animal
Rabbit
Specificity
Mouse, Rat, Human
Clone
CBFYM-1800
Antibody Isotype
IgG
Application
WB, IP, IHC-P, IF

Basic Information

Specificity
Mouse, Rat, Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
METHYL-CPG BINDING DOMAIN PROTEIN 2
Introduction
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain. Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene.
Entrez Gene ID
Human8932
Mouse17191
Rat680172
UniProt ID
HumanQ9UBB5
MouseQ9Z2E1
RatB0BNM2
Alternative Names
Methyl-CpG Binding Domain Protein 2; Demethylase; DMTase; Methyl-CpG-Binding Domain Protein 2; Methyl-CpG-Binding Protein MBD2; NY-CO-41
Function
Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
Biological Process
Aging Source: Ensembl
Cellular response to organic cyclic compound Source: Ensembl
Chromatin remodeling Source: ComplexPortal
DNA methylation-dependent heterochromatin assembly Source: BHF-UCL
Embryonic organ development Source: Ensembl
Heart development Source: Ensembl
Histone deacetylation Source: ComplexPortal
Maternal behavior Source: Ensembl
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of chromatin binding Source: Ensembl
Positive regulation of transcription, DNA-templated Source: ComplexPortal
Positive regulation of Wnt signaling pathway Source: Ensembl
Regulation of cell population proliferation Source: Ensembl
Regulation of DNA methylation Source: Ensembl
Response to estradiol Source: Ensembl
Response to mechanical stimulus Source: Ensembl
Response to nutrient levels Source: Ensembl
Cellular Location
Nucleus
Note: Nuclear, in discrete foci. Detected at replication foci in late S phase.
More Infomation

Lax, E., Do Carmo, S., Enuka, Y., Sapozhnikov, D. M., Welikovitch, L. A., Mahmood, N., ... & Szyf, M. (2023). Methyl-CpG binding domain 2 (Mbd2) is an epigenetic regulator of autism-risk genes and cognition. Translational Psychiatry, 13(1), 259.

Lei, Q., & Zhang, W. (2022). Methyl CpG binding domain protein 2 (MBD2) in inflammation. Chinese Medical Journal, 135(23), 2880-2882.

Wang, Y., Zhang, L., Huang, T., Wu, G. R., Zhou, Q., Wang, F. X., ... & Wang, C. Y. (2022). The methyl-CpG-binding domain 2 facilitates pulmonary fibrosis by orchestrating fibroblast to myofibroblast differentiation. European Respiratory Journal, 60(3).

Wu, J., Huang, Q., Li, Q., Gu, Y., Zhan, Y., Wang, T., ... & Xie, J. (2022). Increased methyl-CpG-binding domain protein 2 promotes cigarette smoke-induced pulmonary hypertension. Frontiers in Oncology, 12, 879793.

Jones, G. R., Brown, S. L., Phythian-Adams, A. T., Ivens, A. C., Cook, P. C., & MacDonald, A. S. (2020). The methyl-CpG-binding protein Mbd2 regulates susceptibility to experimental colitis via control of CD11c+ cells and colonic epithelium. Frontiers in Immunology, 11, 183.

Leighton, G., & Williams Jr, D. C. (2020). The methyl-CpG–Binding domain 2 and 3 proteins and formation of the nucleosome remodeling and deacetylase complex. Journal of molecular biology, 432(6), 1624-1639.

Gong, W., Ni, M., Chen, Z., & Zheng, Z. (2020). Expression and clinical significance of methyl‑CpG binding domain protein 2 in high‑grade serous ovarian cancer. Oncology letters, 20(3), 2749-2756.

Song, X., Zhang, Y., Zhong, Q., Zhan, K., Bi, J., Tang, J., ... & Li, B. (2020). Identification and functional characterization of methyl-CpG binding domain protein from Tribolium castaneum. Genomics, 112(3), 2223-2232.

Pei, Y. F., Xu, X. N., Wang, Z. F., Wang, F. W., Wu, W. D., Geng, J. F., & Liu, X. Q. (2019). Methyl-CpG binding domain protein 2 inhibits the malignant characteristic of lung adenocarcinoma through the epigenetic modulation of 10 to 11 translocation 1 and miR-200s. The American Journal of Pathology, 189(5), 1065-1076.

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For research use only. Not intended for any clinical use.

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