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Rabbit Anti-MAPK7 Monoclonal Antibody (EP791Y) (CBMAB-1202-YC)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
EP791Y
Antibody Isotype
IgG
Application
ICC, FC, IP, WB

Basic Information

Immunogen
Synthetic peptide corresponding to residues in the C-terminus of Human ERK5.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Mitogen-Activated Protein Kinase 7
Introduction
MAPK7 is a member of the MAP kinase family, which is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). In response to extracelluar signals, MAPK7 translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors.
Entrez Gene ID
Human5598
Mouse23939
Rat114509
UniProt ID
HumanQ13164
MouseQ9WVS8
RatP0C865
Alternative Names
BMK1; ERK4; ERK5; PRKM7
Function
Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction.
Biological Process
cAMP-mediated signaling1 PublicationNAS:BHF-UCL
Cell cycleIEA:UniProtKB-KW
Cell differentiationIEA:UniProtKB-KW
Cellular response to growth factor stimulusManual Assertion Based On ExperimentIGI:BHF-UCL
Cellular response to hydrogen peroxideManual Assertion Based On ExperimentIMP:BHF-UCL
Cellular response to laminar fluid shear stressManual Assertion Based On ExperimentIMP:BHF-UCL
Cellular response to transforming growth factor beta stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of calcineurin-NFAT signaling cascadeIEA:Ensembl
Negative regulation of cyclic-nucleotide phosphodiesterase activity1 PublicationNAS:BHF-UCL
Negative regulation of endothelial cell apoptotic processManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of extrinsic apoptotic signaling pathway in absence of ligandManual Assertion Based On ExperimentIGI:BHF-UCL
Negative regulation of heterotypic cell-cell adhesionManual Assertion Based On ExperimentIGI:BHF-UCL
Negative regulation of inflammatory responseManual Assertion Based On ExperimentTAS:BHF-UCL
Negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of response to cytokine stimulusManual Assertion Based On ExperimentIGI:BHF-UCL
Peptidyl-serine phosphorylationIEA:Ensembl
Positive regulation of protein metabolic processManual Assertion Based On ExperimentIGI:BHF-UCL
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIGI:BHF-UCL
Positive regulation of transcription from RNA polymerase II promoter in response to stressManual Assertion Based On ExperimentIMP:BHF-UCL
Regulation of angiogenesisIEA:Ensembl
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Cytoplasm
Nucleus
Nucleus, PML body
Translocates to the nucleus upon activation.
PTM
Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity).
Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
More Infomation

Wu, C., Liu, H., Zhong, D., Yang, X., Liao, Z., Chen, Y., ... & Su, P. (2024). Mapk7 deletion in chondrocytes causes vertebral defects by reducing MEF2C/PTEN/AKT signaling. Genes & Diseases, 11(2), 964-977.

Chen, C. P., Su, T. C., Yang, M. J., Chen, W. T., Siao, A. C., Huang, L. R., ... & Kao, Y. H. (2022). Green tea epigallocatechin gallate suppresses 3T3-L1 cell growth via microRNA-143/MAPK7 pathways. Experimental Biology and Medicine, 247(18), 1670-1679.

Vanchin, B., Sol, M., Gjaltema, R. A., Brinker, M., Kiers, B., Pereira, A. C., ... & Krenning, G. (2021). Reciprocal regulation of endothelial–mesenchymal transition by MAPK7 and EZH2 in intimal hyperplasia and coronary artery disease. Scientific Reports, 11(1), 17764.

Bian, Q., Chen, B., Weng, B., Chu, D., Tang, X., Yan, S., ... & Ran, M. (2021). Circbtbd7 promotes immature porcine sertoli cell growth through modulating mir-24-3p/mapk7 axis to inactivate p38 mapk signaling pathway. International Journal of Molecular Sciences, 22(17), 9385.

Jiao, J., Zhang, M., Yang, P., Huang, Y., Hu, X., Cai, J., ... & Huang, Y. (2020). Identification of de novo JAK2 and MAPK7 mutations related to autism spectrum disorder using whole-exome sequencing in a Chinese child and adolescent trio-based sample. Journal of Molecular Neuroscience, 70, 219-229.

Gao, Y., Ma, H., Gao, Y., Tao, K., Fu, L., Ren, R., ... & Wen, Y. (2020). CircRNA Circ_0001721 promotes the progression of osteosarcoma through miR-372-3p/MAPK7 axis. Cancer Management and Research, 8287-8302.

Green, D., Eyre, H., Singh, A., Taylor, J. T., Chu, J., Jeys, L., ... & Finegan, K. G. (2020). Targeting the MAPK7/MMP9 axis for metastasis in primary bone cancer. Oncogene, 39(33), 5553-5569.

Vanchin, B., Offringa, E., Friedrich, J., Brinker, M. G., Kiers, B., Pereira, A. C., ... & Krenning, G. (2019). MicroRNA‐374b induces endothelial‐to‐mesenchymal transition and early lesion formation through the inhibition of MAPK7 signaling. The Journal of pathology, 247(4), 456-470.

Hou, Y., Feng, H., Jiao, J., Qian, L., Sun, B., Chen, P., ... & Liang, Z. (2019). Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7. Artificial Cells, Nanomedicine, and Biotechnology, 47(1), 2065-2071.

Mao, X., Zhang, J., Liu, W., Yan, S., Liu, Q., Fu, H., ... & Wang, F. (2019). The MKKK62-MKK3-MAPK7/14 module negatively regulates seed dormancy in rice. Rice, 12(1), 1-14.

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For research use only. Not intended for any clinical use.

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