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Rabbit Anti-MAPK10 Recombinant Antibody (CBFYM-1589) (CBMAB-M1755-FY)


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Summary

Host Animal
Rabbit
Specificity
Human, Rat
Clone
CBFYM-1589
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
GST fusion protein corresponding to Full length rat JNK3/SAPK1b
Specificity
Human, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
mitogen-activated protein kinase 10
Introduction
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as integration points for multiple biochemical signals, and thus are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is specifically expressed in a subset of neurons in the nervous system, and is activated by threonine and tyrosine phosphorylation. Targeted deletion of this gene in mice suggests that it may have a role in stress-induced neuronal apoptosis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon.
Entrez Gene ID
Human5602
Rat25272
UniProt ID
HumanP53779
RatP49187
Alternative Names
Mitogen-Activated Protein Kinase 10; Stress-Activated Protein Kinase JNK3; Stress-Activated Protein Kinase 1b; C-Jun N-Terminal Kinase 3; MAP Kinase P49 3F12; MAP Kinase 10; EC 2.7.11.24; PRKM10; SAPK1b
Function
Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the amyloid-beta precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock (PubMed:22441692).
Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed:22327296).
Biological Process
Cellular senescenceTAS:Reactome
Fc-epsilon receptor signaling pathwayTAS:Reactome
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
JNK cascadeBy SimilarityISS:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of circadian rhythmISS:UniProtKB
Regulation of DNA-binding transcription factor activityTAS:Reactome
Response to light stimulusISS:UniProtKB
Rhythmic processIEA:UniProtKB-KW
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Cytoplasm
Membrane
Nucleus
Mitochondrion
Palmitoylation regulates MAPK10 trafficking to cytoskeleton. Recruited to the mitochondria in the presence of SARM1 (By similarity).
Involvement in disease
A chromosomal aberration involving MAPK10 has been found in a single patient with pharmacoresistant epileptic encephalopathy. Translocation t(Y;4)(q11.2;q21) which causes MAPK10 truncation.
PTM
Dually phosphorylated on Thr-221 and Tyr-223 by MAP2K4 and MAP2K7, which activates the enzyme. MAP2K7 shows a strong preference for Thr-221 while MAP2K4 phosphorylates Tyr-223 preferentially. Weakly autophosphorylated on threonine and tyrosine residues in vitro.
Palmitoylation regulates subcellular location and axonal development.
More Infomation

Deng, Y. W., Liu, F., Li, Z. T., Gao, J. H., Zhao, Y., Yang, X. L., & Xia, Y. L. (2022). Hyperglycemia promotes myocardial dysfunction via the ERS-MAPK10 signaling pathway in db/db mice. Laboratory Investigation, 102(11), 1192-1202.

Zhang, Y., Long, Y., Liu, Y., Yang, M., Wang, L., Liu, X., ... & Luo, Y. (2022). MAPK5 and MAPK10 overexpression influences strawberry fruit ripening, antioxidant capacity and resistance to Botrytis cinerea. Planta, 255(1), 19.

Liu, F., Deng, Y., Zhao, Y., Li, Z., Gao, J., Zhang, Y., ... & Xia, Y. (2022). Time series RNA-seq analysis identifies MAPK10 as a critical gene in diabetes mellitus-induced atrial fibrillation in mice. Journal of Molecular and Cellular Cardiology, 168, 70-82.

Gao, Y., Wang, Y., Wang, X., Zhao, C., Wang, F., Du, J., ... & Zhang, J. (2021). miR-335-5p suppresses gastric cancer progression by targeting MAPK10. Cancer Cell International, 21(1), 1-12.

Li, H., Li, Y., Zhang, Y., Tan, B., Huang, T., Xiong, J., ... & Fu, L. (2021). MAPK10 Expression as a Prognostic Marker of the Immunosuppressive Tumor Microenvironment in Human Hepatocellular Carcinoma. Frontiers in Oncology, 11, 687371.

Wu, X., Li, X., & Xu, G. (2020). Propofol suppresses the progression of non‑small cell lung cancer via downregulation of the miR‑21‑5p/MAPK10 axis. Oncology Reports, 44(2), 487-498.

Sun, R., Xiang, T., Tang, J., Peng, W., Luo, J., Li, L., ... & Tao, Q. (2020). 19q13 KRAB zinc-finger protein ZNF471 activates MAPK10/JNK3 signaling but is frequently silenced by promoter CpG methylation in esophageal cancer. Theranostics, 10(5), 2243.

Zhang, L., Li, H., Yuan, M., Li, M., & Zhang, S. (2019). Cervical cancer cells-secreted exosomal microRNA-221-3p promotes invasion, migration and angiogenesis of microvascular endothelial cells in cervical cancer by down-regulating MAPK10 expression. Cancer management and research, 10307-10319.

Xie, Y., Liu, Y., Fan, X., Zhang, L., Li, Q., Li, S., ... & Xiao, Y. (2019). MicroRNA-21 promotes progression of breast cancer via inhibition of mitogen-activated protein kinase10 (MAPK10). Bioscience reports, BSR20181000-BSR20181000.

Liu, J., & Li, Y. (2019). Upregulation of MAPK10, TUBB2B and RASL11B may contribute to the development of neuroblastoma. Molecular medicine reports, 20(4), 3475-3486.

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For research use only. Not intended for any clinical use.

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