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Rabbit Anti-MAP2K7 Recombinant Antibody (CBFYM-0285) (CBMAB-M0350-FY)


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Summary

Host Animal
Rabbit
Specificity
Human
Clone
CBFYM-0285
Antibody Isotype
IgG
Application
IF

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
mitogen-activated protein kinase kinase 7
Introduction
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
5609
UniProt ID
O14733
Alternative Names
Mitogen-Activated Protein Kinase Kinase 7; Stress-Activated Protein Kinase Kinase 4; C-Jun N-Terminal Kinase Kinase 2; JNK-Activating Kinase 2; MAP Kinase Kinase 7; MAPK/ERK Kinase 7; SAPK Kinase 4; EC 2.7.12.2; SAPKK-4; PRKMK7; SAPKK4; MEK 7
Function
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by pro-inflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Part of a non-canonical MAPK signaling pathway, composed of the upstream MAP3K12 kinase and downstream MAP kinases MAPK1/ERK2 and MAPK3/ERK1, that enhances the AP-1-mediated transcription of APP in response to APOE (PubMed:28111074).
Biological Process
Apoptotic processIEA:UniProtKB-KW
Cellular response to sorbitolIEA:Ensembl
Cellular senescenceTAS:Reactome
Fc-epsilon receptor signaling pathwayTAS:Reactome
JNK cascadeISS:BHF-UCL
MAPK cascadeManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of ERK1 and ERK2 cascadeManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of JNK cascadeIEA:Ensembl
Positive regulation of JUN kinase activityISS:BHF-UCL
Positive regulation of neuron apoptotic processIEA:Ensembl
Positive regulation of telomerase activityManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of telomere cappingManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of telomere maintenance via telomeraseManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:UniProtKB
Response to heatManual Assertion Based On ExperimentIDA:UniProtKB
Response to osmotic stressManual Assertion Based On ExperimentIDA:UniProtKB
Response to tumor necrosis factorManual Assertion Based On ExperimentIDA:UniProtKB
Response to UVManual Assertion Based On ExperimentIDA:UniProtKB
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Stress-activated MAPK cascadeManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cytoplasm
Nucleus
PTM
Activated by phosphorylation on Ser-271 and Thr-275 by MAP kinase kinase kinases (MAP3Ks).
More Infomation

Kim, D. R., Orr, M. J., Kwong, A. J., Deibler, K. K., Munshi, H. H., Bridges, C. S., ... & Scheidt, K. A. (2023). Rational Design of Highly Potent and Selective Covalent MAP2K7 Inhibitors. ACS Medicinal Chemistry Letters, 14(5), 606-613.

Scheidt, K., Kim, D., Orr, M., Kwong, A., Deibler, K., Munshi, H., ... & Lacorazza, D. (2023). The Rational Design of Highly Potent and Selective Covalent MAP2K7 Inhibitors.

Hong, Y. H., Aziz, N., Park, J. G., Lee, D., Kim, J. K., Kim, S. A., ... & Cho, J. Y. (2022). The EEF1AKMT3/MAP2K7/TP53 axis suppresses tumor invasiveness and metastasis in gastric cancer. Cancer letters, 544, 215803.

Qi, A., Ru, W., Yang, H., Yang, Y., Tang, J., Yang, S., ... & Chen, H. (2022). Circular RNA ACTA1 acts as a sponge for miR-199a-5p and miR-433 to regulate bovine myoblast development through the MAP3K11/MAP2K7/JNK pathway. Journal of Agricultural and Food Chemistry, 70(10), 3357-3373.

Chen, T. J., Du, W., Junco, J. J., Bridges, C. S., Shen, Y., Puppi, M., ... & Lacorazza, H. D. (2021). Inhibition of the MAP2K7-JNK pathway with 5Z-7-oxozeaenol induces apoptosis in T-cell acute lymphoblastic leukemia. Oncotarget, 12(18), 1787.

Ray, D., Yun, Y. C., Idris, M., Cheng, S., Boot, A., Iain, T. B. H., ... & Epstein, D. M. (2020). A tumor-associated splice-isoform of MAP2K7 drives dedifferentiation in MBNL1-low cancers via JNK activation. Proceedings of the National Academy of Sciences, 117(28), 16391-16400.

Murakawa, Y., Valter, S., Barr, H., London, N., & Kinoshita, T. (2020). Structural basis for producing selective MAP2K7 inhibitors. Bioorganic & Medicinal Chemistry Letters, 30(22), 127546.

Openshaw, R. L., Thomson, D. M., Thompson, R., Penninger, J. M., Pratt, J. A., Morris, B. J., & Dawson, N. (2020). Map2k7 haploinsufficiency induces brain imaging endophenotypes and behavioral phenotypes relevant to schizophrenia. Schizophrenia Bulletin, 46(1), 211-223.

Chen, T. J., Shen, Y., Bridges, C. S., Park, C. S., Junco, J. J., Rabin, K. R., & Lacorazza, H. D. (2019). Pharmacological Inhibition of MAP2K7 Induces Apoptosis and Cell Cycle Arrest in T-Cell Acute Lymphoblastic Leukemia. Blood, 134, 3889.

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For research use only. Not intended for any clinical use.

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