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Mouse Anti-MAFG Recombinant Antibody (CBFYM-1285) (CBMAB-M1444-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-1285
Antibody Isotype
IgG2a
Application
WB

Basic Information

Immunogen
E. coli-derived rhMafG
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
MAFG
Introduction
Globin gene expression is regulated through nuclear factor erythroid-2 elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters. NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein and the tissue-restricted protein p45 NFE2. Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper proteins.
Entrez Gene ID
4097
UniProt ID
O15525
Alternative Names
MAF BZIP Transcription Factor G; V-Maf Avian Musculoaponeurotic Fibrosarcoma Oncogene Homolog G; Basic Leucine Zipper Transcription Factor MafG; Transcription Factor MafG; HMAF; V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog G
Function
Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed:11154691).
However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1 and NFE2L2, and recruiting them to specific DNA-binding sites (PubMed:8932385, PubMed:9421508, PubMed:11154691).
Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NFE2L2 transcription factor (PubMed:11154691).
Transcription factor, component of erythroid-specific transcription factor NFE2L2 (PubMed:11154691).
Activates globin gene expression when associated with NFE2L2 (PubMed:11154691).
May be involved in signal transduction of extracellular H(+) (By similarity).
Biological Process
Adult behaviorIEA:Ensembl
In utero embryonic developmentIEA:Ensembl
Positive regulation of gene expressionIEA:Ensembl
Regulation of cell population proliferationIEA:Ensembl
Regulation of cellular pHIEA:Ensembl
Regulation of epidermal cell differentiationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus
PTM
Acetylated in erythroid cells by CREB-binding protein (CBP). Acetylation augments the DNA-binding activity of NFE2, but has no effect on binding NFE2.
Sumoylation at Lys-14 is required for active transcriptional repression.
More Infomation

Dabbaghi, K. G., Mashatan, N., Faraz, O., Bashkandi, A. H., Shomoossi, N., & Tabnak, P. (2023). A review on the roles and molecular mechanisms of MAFG-AS1 in oncogenesis. Pathology-Research and Practice, 154348.

Patel, S. D., Anand, D., Motohashi, H., Katsuoka, F., Yamamoto, M., & Lachke, S. A. (2022). Deficiency of the bZIP transcription factors Mafg and Mafk causes misexpression of genes in distinct pathways and results in lens embryonic developmental defects. Frontiers in Cell and Developmental Biology, 10, 981893.

Ye, C., Qin, S., Guo, F., Yang, Y., Wang, H., Zhang, C., & Yang, B. (2022). LncRNA EIF3J-AS1 functions as an oncogene by regulating MAFG to promote prostate cancer progression. Journal of Cancer, 13(1), 146.

Jia, H., Wu, D., Zhang, Z., & Li, S. (2021). Regulatory effect of the MAFG‑AS1/miR‑150‑5p/MYB axis on the proliferation and migration of breast cancer cells. International Journal of Oncology, 58(1), 33-44.

Ye, L., Feng, W., Weng, H., Yuan, C., Liu, J., & Wang, Z. (2020). MAFG-AS1 aggravates the progression of pancreatic cancer by sponging miR-3196 to boost NFIX. Cancer Cell International, 20(1), 1-10.

Jia, Y. C., Wang, J. Y., Liu, Y. Y., Li, B., Guo, H., & Zang, A. M. (2019). LncRNA MAFG‐AS1 facilitates the migration and invasion of NSCLC cell via sponging miR‐339‐5p from MMP15. Cell Biology International, 43(4), 384-393.

Sui, Y., Lin, G., Zheng, Y., & Huang, W. (2019). LncRNA MAFG-AS1 boosts the proliferation of lung adenocarcinoma cells via regulating miR-744-5p/MAFG axis. European Journal of Pharmacology, 859, 172465.

Katsuoka, F., Otsuki, A., Takahashi, M., Ito, S., & Yamamoto, M. (2019). Direct and specific functional evaluation of the Nrf2 and MafG heterodimer by introducing a tethered dimer into small Maf-deficient cells. Molecular and cellular biology, 39(20), e00273-19.

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For research use only. Not intended for any clinical use.

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