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Mouse Anti-LIN28A Recombinant Antibody (CBYJL-1742) (CBMAB-L1647-YJ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJL-1742
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
LIN28 antibody was raised in Rabbit using a purified recombinant human LIN28 protein fragments expressed in E. coli as the immunogen.
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
LIN28A
Introduction
LIN28A is a LIN-28 family RNA-binding protein that acts as a posttranscriptional regulator of genes involved in developmental timing and self-renewal in embryonic stem cells. LIN28A functions through direct interaction with target mRNAs and by disrupting the maturation of certain miRNAs involved in embryonic development. This protein prevents the terminal processing of the LET7 family of microRNAs which are major regulators of cellular growth and differentiation. Aberrant expression of this gene is associated with cancer progression in multiple tissues. Diseases associated with LIN28A include Embryonal Tumor With Multilayered Rosettes and Central Nervous System Primitive Neuroectodermal Neoplasm. Among its related pathways are Translational Control and Wnt / Hedgehog / Notch.
Entrez Gene ID
79727
UniProt ID
Q9H9Z2
Alternative Names
CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A
Function
RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism (PubMed:21247876).
Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Suppressor of microRNA (miRNA) biogenesis, including that of let-7, miR107, miR-143 and miR-200c. Specifically binds the miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in pre-miRNA terminal loop, and recruits TUT4 and TUT7 uridylyltransferases (PubMed:18951094, PubMed:19703396, PubMed:22118463, PubMed:22898984).
This results in the terminal uridylation of target pre-miRNAs (PubMed:18951094, PubMed:19703396, PubMed:22118463, PubMed:22898984).
Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state by preventing let-7-mediated differentiation of embryonic stem cells (PubMed:18951094, PubMed:19703396, PubMed:22118463, PubMed:22898984).
Localized to the periendoplasmic reticulum area, binds to a large number of spliced mRNAs and inhibits the translation of mRNAs destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Binds to and enhances the translation of mRNAs for several metabolic enzymes, such as PFKP, PDHA1 or SDHA, increasing glycolysis and oxidative phosphorylation. Which, with the let-7 repression may enhance tissue repair in adult tissue (By similarity).
Biological Process
Cellular response to glucose stimulusISS:BHF-UCL
Germ cell developmentIEA:Ensembl
miRNA catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of glial cell differentiationIEA:Ensembl
Negative regulation of translationISS:UniProtKB
Positive regulation of cell proliferation involved in kidney developmentIEA:Ensembl
Positive regulation of cytoplasmic translationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of neuron differentiationIEA:Ensembl
Positive regulation of production of miRNAs involved in gene silencing by miRNAISS:BHF-UCL
Positive regulation of protein kinase B signalingISS:BHF-UCL
Positive regulation of TOR signalingISS:BHF-UCL
Pre-miRNA processingManual Assertion Based On ExperimentIMP:UniProtKB
RNA 3' uridylationIEA:Ensembl
RNA 3'-end processingManual Assertion Based On ExperimentIMP:UniProtKB
Stem cell differentiationIEA:Ensembl
Stem cell population maintenanceManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Cytoplasm
Rough endoplasmic reticulum
Cytoplasm, P-body
Cytoplasm, Stress granule
Nucleus, nucleolus
Predominantly cytoplasmic (PubMed:22118463).
In the cytoplasm, localizes to peri-endoplasmic reticulum regions and detected in the microsomal fraction derived from rough endoplasmic reticulum (RER) following subcellular fractionation. May be bound to the cytosolic surface of RER on which ER-associated mRNAs are translated (By similarity).
Shuttle from the nucleus to the cytoplasm requires RNA-binding (PubMed:17617744).
Nucleolar localization is observed in 10-15% of the nuclei in differentiated myotubes (By similarity).
More Infomation

Wu, K., Ahmad, T., & Eri, R. (2022). LIN28A: A multifunctional versatile molecule with future therapeutic potential. World journal of biological chemistry, 13(2), 35.

Jouan, Y., Bouchemla, Z., Bardèche-Trystram, B., Sana, J., Andrique, C., Ea, H. K., ... & Hay, E. (2022). Lin28a induces SOX9 and chondrocyte reprogramming via HMGA2 and blunts cartilage loss in mice. Science Advances, 8(34), eabn3106.

Nathan, F. M., Ohtake, Y., Wang, S., Jiang, X., Sami, A., Guo, H., ... & Li, S. (2020). Upregulating Lin28a promotes axon regeneration in adult mice with optic nerve and spinal cord injury. Molecular Therapy, 28(8), 1902-1917.

Jung, G. S., Hwang, Y. J., Choi, J. H., & Lee, K. M. (2020). Lin28a attenuates TGF-β-induced renal fibrosis. BMB reports, 53(11), 594.

Lu, J., Liu, X., Zheng, J., Song, J., Liu, Y., Ruan, X., ... & Xue, Y. (2020). Lin28A promotes IRF6-regulated aerobic glycolysis in glioma cells by stabilizing SNHG14. Cell death & disease, 11(6), 447.

Zhang, J., Xu, A., Miao, C., Yang, J., Gu, M., & Song, N. (2019). Prognostic value of Lin28A and Lin28B in various human malignancies: a systematic review and meta-analysis. Cancer cell international, 19(1), 1-8.

Haq, S., Das, S., Kim, D. H., Chandrasekaran, A. P., Hong, S. H., Kim, K. S., & Ramakrishna, S. (2019). The stability and oncogenic function of LIN28A are regulated by USP28. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1865(3), 599-610.

Sang, H., Wang, D., Zhao, S., Zhang, J., Zhang, Y., Xu, J., ... & Liu, N. (2019). Dppa3 is critical for Lin28a-regulated ES cells naïve–primed state conversion. Journal of Molecular Cell Biology, 11(6), 474-488.

Ma, H., Yu, S., Liu, X., Zhang, Y., Fakadej, T., Liu, Z., ... & Liu, J. (2019). Lin28a regulates pathological cardiac hypertrophic growth through Pck2-mediated enhancement of anabolic synthesis. Circulation, 139(14), 1725-1740.

Xia, X., Teotia, P., & Ahmad, I. (2018). Lin28a regulates neurogliogenesis in mammalian retina through the Igf signaling. Developmental biology, 440(2), 113-128.

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For research use only. Not intended for any clinical use.

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