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Rat Anti-KPNA1 Recombinant Antibody (2D9) (CBMAB-K1466-LY)


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Summary

Host Animal
Rat
Specificity
Mouse
Clone
2D9
Antibody Isotype
IgG2a
Application
ICC, WB

Basic Information

Immunogen
Recombinant Mouse full length Importin alpha5.
Specificity
Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
1 mg/mL
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
karyopherin alpha 1 (importin alpha 5)
Introduction
The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. This protein interacts with the recombination activating gene 1 (RAG1) protein and is a putative substrate of the RAG1 ubiquitin ligase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
Entrez Gene ID
16646
UniProt ID
Q60960
Alternative Names
AW494490; IPOA5; NPI1; Rch2; mSRP1
Function
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
Biological Process
NLS-bearing protein import into nucleusManual Assertion Based On ExperimentIBA:GO_Central
Postsynapse to nucleus signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Regulation of apoptotic processIEA:Ensembl
Regulation of canonical Wnt signaling pathwayIEA:Ensembl
Regulation of DNA recombinationManual Assertion Based On ExperimentTAS:ProtInc
Satellite cell activation involved in skeletal muscle regenerationIEA:Ensembl
Skeletal muscle satellite cell proliferationIEA:Ensembl
Cellular Location
Cytoplasm
Nucleus
PTM
Polyubiquitinated in the presence of RAG1 (in vitro).
More Infomation

Song, J., Guo, Y., Wang, D., Quan, R., Wang, J., & Liu, J. (2023). Seneca Valley virus 3Cpro antagonizes type I interferon response by targeting STAT1-STAT2-IRF9 and KPNA1 signals. Journal of Virology, 97(10), e00727-23.

PLOS ONE Editors. (2023). Expression of Concern: Effects of Importin α1/KPNA1 deletion and adolescent social isolation stress on psychiatric disorder-associated behaviors in mice. Plos one, 18(1), e0280124.

Zhang, J., Yuan, S., Peng, Q., Ding, Z., Hao, W., Peng, G., ... & Fang, L. (2022). Porcine epidemic diarrhea virus nsp7 inhibits interferon-induced JAK-STAT signaling through sequestering the interaction between KPNA1 and STAT1. Journal of Virology, 96(9), e00400-22.

Tsoi, H., Man, E. P., Leung, M. H., Mok, K. C., Chau, K. M., Wong, L. S., ... & Khoo, U. S. (2021). KPNA1 regulates nuclear import of NCOR2 splice variant BQ323636. 1 to confer tamoxifen resistance in breast cancer. Clinical and Translational Medicine, 11(10).

Sakurai, K., Itou, T., Morita, M., Kasahara, E., Moriyama, T., Macpherson, T., ... & Hikida, T. (2021). Effects of Importin α1/KPNA1 deletion and adolescent social isolation stress on psychiatric disorder-associated behaviors in mice. Plos one, 16(11), e0258364.

Mok, K. C., Tsoi, H., Chou, K. M., Khoo, U. S., & Man, P. S. (2018). KPNA1 mediates the nuclear import of BQ323636. 1 and confer tamoxifen resistance in breast cancer cells. In Proceedings of the Annual Meeting of the American Association for Cancer Research. American Association for Cancer Research. The Journal's web site is located at http://cancerres. aacrjournals. org/.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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