Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-KPNA1 Recombinant Antibody (114-E12) (CBMAB-K1463-LY)


See all KPNA1 antibodies
Online Inquiry

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
114-E12
Antibody Isotype
IgG1
Application
ELISA, IF, IP, WB

Basic Information

Immunogen
GST fusion protein
Specificity
Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Preservative
0.1% sodium azide
Concentration
0.5 mg/mL
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
karyopherin alpha 1 (importin alpha 5)
Introduction
The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. This protein interacts with the recombination activating gene 1 (RAG1) protein and is a putative substrate of the RAG1 ubiquitin ligase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
Entrez Gene ID
Human3836
Mouse16646
UniProt ID
HumanP52294
MouseQ60960
Function
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
Biological Process
NLS-bearing protein import into nucleusManual Assertion Based On ExperimentIBA:GO_Central
Postsynapse to nucleus signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Regulation of apoptotic processIEA:Ensembl
Regulation of canonical Wnt signaling pathwayIEA:Ensembl
Regulation of DNA recombinationManual Assertion Based On ExperimentTAS:ProtInc
Satellite cell activation involved in skeletal muscle regenerationIEA:Ensembl
Skeletal muscle satellite cell proliferationIEA:Ensembl
Cellular Location
Cytoplasm
Nucleus
PTM
Polyubiquitinated in the presence of RAG1 (in vitro).
More Infomation

Song, J., Guo, Y., Wang, D., Quan, R., Wang, J., & Liu, J. (2023). Seneca Valley virus 3Cpro antagonizes type I interferon response by targeting STAT1-STAT2-IRF9 and KPNA1 signals. Journal of Virology, 97(10), e00727-23.

PLOS ONE Editors. (2023). Expression of Concern: Effects of Importin α1/KPNA1 deletion and adolescent social isolation stress on psychiatric disorder-associated behaviors in mice. Plos one, 18(1), e0280124.

Zhang, J., Yuan, S., Peng, Q., Ding, Z., Hao, W., Peng, G., ... & Fang, L. (2022). Porcine epidemic diarrhea virus nsp7 inhibits interferon-induced JAK-STAT signaling through sequestering the interaction between KPNA1 and STAT1. Journal of Virology, 96(9), e00400-22.

Tsoi, H., Man, E. P., Leung, M. H., Mok, K. C., Chau, K. M., Wong, L. S., ... & Khoo, U. S. (2021). KPNA1 regulates nuclear import of NCOR2 splice variant BQ323636. 1 to confer tamoxifen resistance in breast cancer. Clinical and Translational Medicine, 11(10).

Sakurai, K., Itou, T., Morita, M., Kasahara, E., Moriyama, T., Macpherson, T., ... & Hikida, T. (2021). Effects of Importin α1/KPNA1 deletion and adolescent social isolation stress on psychiatric disorder-associated behaviors in mice. Plos one, 16(11), e0258364.

Mok, K. C., Tsoi, H., Chou, K. M., Khoo, U. S., & Man, P. S. (2018). KPNA1 mediates the nuclear import of BQ323636. 1 and confer tamoxifen resistance in breast cancer cells. In Proceedings of the Annual Meeting of the American Association for Cancer Research. American Association for Cancer Research. The Journal's web site is located at http://cancerres. aacrjournals. org/.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-KPNA1 Recombinant Antibody (114-E12)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quoteOnline Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry