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Mouse Anti-KLF5 Recombinant Antibody (2G12) (CBMAB-K1116-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
2G12
Antibody Isotype
IgG2b, κ
Application
ELISA, WB

Basic Information

Immunogen
KLF5 (NP_001721, 358 a.a. ~ 457 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: RYNRRSNPDL EKRRIHYCDY PGCTKVYTKS SHLKAHLRTH TGEKPYKCTW EGCDWRFARS DELTRHYRKH TGAKPFQCGV CNRSFSRSDH LALHMKRHQN
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
KLF5
Introduction
This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
Entrez Gene ID
688
UniProt ID
Q13887
Alternative Names
Kruppel Like Factor 5; Intestinal-Enriched Krueppel-Like Factor; Kruppel-Like Factor 5 (Intestinal); (Intestinal Kruppel-Like Factor; Colon Krueppel-Like Factor; Transcription Factor BTEB2; BTE-Binding Protein 2; BTEB2; CKLF; IKLF;
Function
Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.
Biological Process
AngiogenesisIEA:Ensembl
Cell-cell signaling via exosomeManual Assertion Based On ExperimentIDA:BHF-UCL
Cellular response to leukemia inhibitory factorIEA:Ensembl
Intestinal epithelial cell developmentIEA:Ensembl
Microvillus assemblyIEA:Ensembl
Myotube differentiation involved in skeletal muscle regenerationIEA:Ensembl
Negative regulation of transcription by RNA polymerase IIIEA:Ensembl
Positive regulation of fat cell differentiationIEA:Ensembl
Positive regulation of pri-miRNA transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:NTNU_SB
Positive regulation of transcription by transcription factor localizationIEA:Ensembl
Regulation of microvillus assemblyIEA:Ensembl
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Satellite cell activation involved in skeletal muscle regenerationIEA:Ensembl
Skeletal muscle satellite cell differentiationIEA:Ensembl
Cellular Location
Nucleus
PTM
Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
More Infomation

Palioura, D., Lazou, A., & Drosatos, K. (2022). Krüppel-like factor (KLF) 5: An emerging foe of cardiovascular health. Journal of Molecular and Cellular Cardiology, 163, 56-66.

Jiang, D., Qiu, T., Peng, J., Li, S., Tala, Ren, W., ... & Chen, C. (2022). YB-1 is a positive regulator of KLF5 transcription factor in basal-like breast cancer. Cell Death & Differentiation, 29(6), 1283-1295.

Li, J. C., Chen, Q. H., Jian, R., Zhou, J. R., Xu, Y., Lu, F., ... & Zhang, H. (2021). The partial role of KLF4 and KLF5 in gastrointestinal tumors. Gastroenterology Research and Practice, 2021.

Jia, X., Chen, H., Ren, Y., Gao, N., Feng, H., Huang, W., ... & Yu, H. (2021). BAP1 antagonizes WWP1-mediated transcription factor KLF5 ubiquitination and inhibits autophagy to promote melanoma progression. Experimental Cell Research, 402(1), 112506.

Ma, J. B., Bai, J. Y., Zhang, H. B., Jia, J., Shi, Q., Yang, C., ... & Guo, P. (2020). KLF5 inhibits STAT3 activity and tumor metastasis in prostate cancer by suppressing IGF1 transcription cooperatively with HDAC1. Cell death & disease, 11(6), 466.

An, T., Dong, T., Zhou, H., Chen, Y., Zhang, J., Zhang, Y. U., ... & Yang, X. (2019). The transcription factor Krüppel-like factor 5 promotes cell growth and metastasis via activating PI3K/AKT/Snail signaling in hepatocellular carcinoma. Biochemical and biophysical research communications, 508(1), 159-168.

Azami, T., Matsumoto, K., Jeon, H., Waku, T., Muratani, M., Niwa, H., ... & Ema, M. (2018). Klf5 suppresses ERK signaling in mouse pluripotent stem cells. PLoS One, 13(11), e0207321.

Zhang, X., Choi, P. S., Francis, J. M., Gao, G. F., Campbell, J. D., Ramachandran, A., ... & Meyerson, M. (2018). Somatic superenhancer duplications and hotspot mutations lead to oncogenic activation of the KLF5 transcription factor. Cancer discovery, 8(1), 108-125.

Ma, Y., Wang, Q., Liu, F., Ma, X., Wu, L., Guo, F., ... & Qin, G. (2018). KLF5 promotes the tumorigenesis and metastatic potential of thyroid cancer cells through the NF-κB signaling pathway. Oncology reports, 40(5), 2608-2618.

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For research use only. Not intended for any clinical use.

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