Rabbit Anti-IRX3 Recombinant Antibody (BA0099) (CBMAB-0394CQ)

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Datasheet

Summary

Host Animal

Rabbit

Specificity

Human, Mouse, Rat

Clone

BA0099

Antibody Isotype

IgG

Application

FC, WB

Basic Information

Immunogen

Human Iroquois homeobox protein 3 aa 50-150 (Cysteine residue)

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

iroquois homeobox 3

Introduction

IRX3 is a member of the Iroquois homeobox gene family and plays a role in an early step of neural development. Members of this family appear to play multiple roles during pattern formation of vertebrate embryos. Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells. IRX3 is a member of the Iroquois homeobox gene family (see IRX1; MIM 606197) and plays a role in an early step of neural development. Members of this family appear to play multiple roles during pattern formation of vertebrate embryos.

Entrez Gene ID

Human	79191
Mouse	16373
Rat	307721

UniProt ID

Human	P78415
Mouse	P81067
Rat	D3ZNE0

Alternative Names

IRX-1; IRXB1

Function

Transcription factor involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells. Acts as a regulator of energy metabolism.

Biological Process

Atrioventricular bundle cell differentiationISS:BHF-UCL
Cell developmentManual Assertion Based On ExperimentIBA:GO_Central
Energy homeostasisISS:UniProtKB
His-Purkinje system cell differentiationISS:BHF-UCL
Mesoderm developmentIEA:Ensembl
Metanephros developmentIEA:Ensembl
Negative regulation of neuron differentiationIEA:Ensembl
Negative regulation of transcription by RNA polymerase IIBy SimilarityISS:BHF-UCL
Neuron differentiationManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of gap junction assemblyBy SimilarityISS:BHF-UCL
Positive regulation of neuron differentiationIEA:Ensembl
Positive regulation of transcription by RNA polymerase IIBy SimilarityISS:BHF-UCL
Purkinje myocyte developmentISS:BHF-UCL
Regulation of cell communication by electrical coupling involved in cardiac conductionBy SimilarityISS:BHF-UCL
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Specification of loop of Henle identityIEA:Ensembl

Cellular Location

Nucleus

More Infomation

References

Narwidina, A., Miyazaki, A., Iwata, K., Kurogoushi, R., Sugimoto, A., Kudo, Y., ... & Iwamoto, T. (2023). Iroquois homeobox 3 regulates odontoblast proliferation and differentiation mediated by Wnt5a expression. Biochemical and biophysical research communications, 650, 47-54.

Tariq, N., Ali, H., & Kausar, T. (2023). Role of FTO (Fat Mass and Obesity-Associated) and IRX3 (Iroquois Homeobox 3) gene variants on obesity in Quetta. Obesity Medicine, 39, 100478.

Brown, R. M., Wang, L., Fu, A., Kannan, A., Mussar, M., Bagchi, I. C., & Jorgensen, J. S. (2022). Irx3 promotes gap junction communication between uterine stromal cells to regulate vascularization during embryo implantation. Biology of reproduction, 106(5), 1000-1010.

de Araújo, T. M., & Velloso, L. A. (2020). Hypothalamic IRX3: a new player in the development of obesity. Trends in Endocrinology & Metabolism, 31(5), 368-377.

Holmquist Mengelbier, L., Lindell‐Munther, S., Yasui, H., Jansson, C., Esfandyari, J., Karlsson, J., ... & Gisselsson, D. (2019). The Iroquois homeobox proteins IRX3 and IRX5 have distinct roles in Wilms tumour development and human nephrogenesis. The Journal of pathology, 247(1), 86-98.

Gholamalizadeh, M., Doaei, S., Akbari, M. E., Rezaei, S., & Jarrahi, A. M. (2018). Influence of fat mass-and obesity-associated genotype, body mass index, and dietary intake on effects of iroquois-related homeobox 3 gene on body weight. Chinese medical journal, 131(17), 2112-2113.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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