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Mouse Anti-HMGA1 Antibody (1B6) (CBMAB-0476-YC)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
1B6
Antibody Isotype
IgG2b
Application
WB, MA

Basic Information

Immunogen
Recombinant protein
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
High Mobility Group AT-Hook 1
Introduction
HMGA1 (high mobility group AT-hook 1) is a chromatin-associated protein involved in the regulation of gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. HMGA1 preferentially binds to the minor groove of AT-rich regions in double-stranded DNA.
Entrez Gene ID
3159
UniProt ID
P17096
Alternative Names
HMG-R; HMGIY; HMGA1A
Function
HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
Biological Process
Base-excision repair Source: UniProtKB
DNA unwinding involved in DNA replication Source: UniProtKB
Negative regulation of cell population proliferation Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Nucleosome disassembly Source: UniProtKB
Oncogene-induced cell senescence Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Regulation of transcription, DNA-templated Source: GO_Central
Cellular Location
Nucleus; Chromosome
Involvement in disease
A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51B.
PTM
Constitutively phosphorylated on two or three sites. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoforms HMG-I and HMG-Y can be phosphorylated by HIPK2. Phosphorylation of HMG-I at Ser-36, Thr-53 and Thr-78 and of HMG-Y at Thr-42 and Thr-67 by HIPK2 modulates DNA-binding affinity.
HMG-Y is not methylated.
Methylation at Arg-58 is mutually exclusive with methylation at Arg-60.
More Infomation

Chia, L., Wang, B., Kim, J. H., Luo, L. Z., Shuai, S., Herrera, I., ... & Resar, L. (2023). HMGA1 induces FGF19 to drive pancreatic carcinogenesis and stroma formation. The Journal of Clinical Investigation, 133(6).

Yang, Q., Wang, Y., Li, M., Wang, Z., Zhang, J., Dai, W., ... & Wang, J. (2021). HMGA1 promotes gastric cancer growth and metastasis by transactivating SUZ12 and CCDC43 expression. Aging (Albany NY), 13(12), 16043.

Hou, P., Meng, S., Li, M., Lin, T., Chu, S., Li, Z., ... & Bai, J. (2021). LINC00460/DHX9/IGF2BP2 complex promotes colorectal cancer proliferation and metastasis by mediating HMGA1 mRNA stability depending on m6A modification. Journal of Experimental & Clinical Cancer Research, 40(1), 1-18.

Zanin, R., Pegoraro, S., Ros, G., Ciani, Y., Piazza, S., Bossi, F., ... & Manfioletti, G. (2019). HMGA1 promotes breast cancer angiogenesis supporting the stability, nuclear localization and transcriptional activity of FOXM1. Journal of Experimental & Clinical Cancer Research, 38, 1-23.

Wei, F., Zhang, T., Deng, S. C., Wei, J. C., Yang, P., Wang, Q., ... & Cao, J. (2019). PD-L1 promotes colorectal cancer stem cell expansion by activating HMGA1-dependent signaling pathways. Cancer letters, 450, 1-13.

Wang, Y., Hu, L., Zheng, Y., & Guo, L. (2019). HMGA1 in cancer: Cancer classification by location. Journal of Cellular and Molecular Medicine, 23(4), 2293-2302.

Méndez, O., Pérez, J., Soberino, J., Racca, F., Cortés, J., & Villanueva, J. (2019). Clinical implications of extracellular HMGA1 in breast cancer. International Journal of Molecular Sciences, 20(23), 5950.

Chiefari, E., Foti, D. P., Sgarra, R., Pegoraro, S., Arcidiacono, B., Brunetti, F. S., ... & Brunetti, A. (2018). Transcriptional regulation of glucose metabolism: the emerging role of the HMGA1 chromatin factor. Frontiers in endocrinology, 9, 357.

Méndez, O., Peg, V., Salvans, C., Pujals, M., Fernández, Y., Abasolo, I., ... & Villanueva, J. (2018). Extracellular HMGA1 promotes tumor invasion and metastasis in triple-negative breast cancer. Clinical Cancer Research, 24(24), 6367-6382.

Resar, L., Chia, L., & Xian, L. (2018). Lessons from the crypt: HMGA1—Amping up Wnt for stem cells and tumor progression. Cancer research, 78(8), 1890-1897.

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For research use only. Not intended for any clinical use.

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