Mouse Anti-HESX1 Antibody (1A11) (CBMAB-0461-YC)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1A11

Antibody Isotype

IgG1

Application

IP, MA

Basic Information

Immunogen

Recombinant peptide

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

HESX1

Introduction

HESX1 (HESX homeobox 1) is a conserved homeobox protein that is a transcriptional repressor in the developing forebrain and pituitary gland. Mutations in HESX1 gene are associated with septooptic dysplasia, HESX1-related growth hormone deficiency, and combined pituitary hormone deficiency.

Entrez Gene ID

8820

UniProt ID

Q9UBX0

Alternative Names

ANF; RPX

Function

Required for the normal development of the forebrain, eyes and other anterior structures such as the olfactory placodes and pituitary gland. Possible transcriptional repressor. Binds to the palindromic PIII sequence, 5'-AGCTTGAGTCTAATTGAATTAACTGTAC-3'. HESX1 and PROP1 bind as heterodimers on this palindromic site, and, in vitro, HESX1 can antagonize PROP1 activation.

Biological Process

Brain development Source: ProtInc
Camera-type eye development Source: Ensembl
Canonical Wnt signaling pathway Source: Ensembl
Cellular response to cadmium ion Source: Ensembl
ERK1 and ERK2 cascade Source: Ensembl
Forebrain morphogenesis Source: Ensembl
Gene expression Source: Ensembl
Gonad development Source: Ensembl
Leukemia inhibitory factor signaling pathway Source: Ensembl
Multicellular organism growth Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: NTNU_SB
Nose development Source: Ensembl
Otic vesicle formation Source: Ensembl
Pituitary gland development Source: UniProtKB
Regulation of embryonic development Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Stem cell differentiation Source: Ensembl
Stem cell population maintenance Source: Ensembl
Thyroid gland development Source: Ensembl

Cellular Location

Nucleus

Involvement in disease

Septooptic dysplasia (SOD):
A clinically heterogeneous disorder defined by any combination of optic nerve hypoplasia, pituitary gland hypoplasia with panhypopopituitarism, and midline abnormalities of the brain, including absence of the corpus callosum and septum pellucidum.
Growth hormone deficiency with pituitary anomalies (GHDPA):
A disease characterized by low or absent growth hormone levels, in the presence of a hypoplastic anterior pituitary lobe and ectopic or absent posterior pituitary lobe.
Pituitary hormone deficiency, combined, 5 (CPHD5):
Combined pituitary hormone deficiency is defined as the impaired production of growth hormone and one or more of the other five anterior pituitary hormones. CPHD5 is characterized by complete or partial deficiencies of growth hormone, thyroid-stimulating hormone, luteinizing hormone, follicle stimulating hormone and adrenocorticotropic hormone.

More Infomation

References

Tong, R., Wang, H., Jin, Y., & Li, H. (2022). Transcription factor HESX1 enhances mesendodermal commitment of human embryonic stem cells by modulating ERK1/2 signaling. Biochemical and Biophysical Research Communications, 619, 27-33.

Alesi, V., Dentici, M. L., Genovese, S., Loddo, S., Bellacchio, E., Orlando, V., ... & Novelli, A. (2021). Homozygous HESX1 and COL1A1 Gene Variants in a Boy with Growth Hormone Deficiency and Early Onset Osteoporosis. International Journal of Molecular Sciences, 22(2), 750.

Zhang, H., Wang, H., Ma, W., Jia, C., Ma, X., Wu, X., ... & Liang, C. (2019). Association analysis of Hesx1 gene polymorphism and growth traits in polled yak. Journal of Henan Agricultural Sciences, 48(8), 134-139.

Pozzi, S., Bowling, S., Apps, J., Brickman, J. M., Rodriguez, T. A., & Martinez-Barbera, J. P. (2019). Genetic deletion of Hesx1 promotes exit from the pluripotent state and impairs developmental diapause. Stem Cell Reports, 13(6), 970-979.

Coutinho, E., Brandão, C. M., & Lemos, M. C. (2019). Combined pituitary hormone deficiency caused by a synonymous HESX1 gene mutation. The Journal of Clinical Endocrinology & Metabolism, 104(7), 2851-2854.

Martynova, N. Y., Eroshkin, F. M., Оrlov, E. E., & Zaraisky, A. G. (2018). HMG-Box factor SOXD/SOX15 and homeodomain-containing factor XANF1/HESX1 directly interact and regulate the expression of XANF1/HESX1 during early forebrain development in Xenopus laevis. Gene, 638, 52-59.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-HESX1 (AA 1-111) Recombinant Antibody (CBFYH-0970)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Datasheet
SDS
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Second antibody and isotype control

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