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Mouse Anti-HCK Recombinant Antibody (1D9-1A9) (CBMAB-A3765-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
1D9-1A9
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
HCK (AAH14435, 1 a.a. ~ 505 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
HCK Proto-Oncogene, Src Family Tyrosine Kinase
Introduction
The protein encoded by this gene is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization. [provided by RefSeq]
Entrez Gene ID
3055
UniProt ID
P08631
Alternative Names
JTK9
Function
Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.
Biological Process
Cell adhesion Source: UniProtKB
Cell differentiation Source: GO_Central
Cytokine-mediated signaling pathway Source: UniProtKB
Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
Inflammatory response Source: UniProtKB-KW
Innate immune response Source: GO_Central
Innate immune response-activating signal transduction Source: UniProtKB
Integrin-mediated signaling pathway Source: UniProtKB
Interferon-gamma-mediated signaling pathway Source: UniProtKB
Leukocyte degranulation Source: UniProtKB
Leukocyte migration involved in immune response Source: UniProtKB
Lipopolysaccharide-mediated signaling pathway Source: UniProtKB
Mesoderm development Source: ProtInc
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of inflammatory response to antigenic stimulus Source: Reactome
Peptidyl-tyrosine phosphorylation Source: UniProtKB
Positive regulation of actin cytoskeleton reorganization Source: UniProtKB
Positive regulation of actin filament polymerization Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Protein autophosphorylation Source: UniProtKB
Protein phosphorylation Source: ProtInc
Regulation of cell shape Source: UniProtKB
Regulation of DNA-binding transcription factor activity Source: UniProtKB
Regulation of inflammatory response Source: UniProtKB
Regulation of phagocytosis Source: UniProtKB
Regulation of podosome assembly Source: UniProtKB
Respiratory burst after phagocytosis Source: UniProtKB
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Cellular Location
Isoform 1: Cytosol; Podosome membrane; Lysosome; Membrane. Associated with specialized secretory lysosomes called azurophil granules. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated.
Isoform 2: Golgi apparatus; Cytoskeleton; Nucleus; Lysosome; Cell membrane; Caveola; Focal adhesion; Cytoplasmic vesicle. 20% of this isoform is associated with caveolae. Localization at the cell membrane and at caveolae requires palmitoylation at Cys-3. Colocalizes with the actin cytoskeleton at focal adhesions.
Cytosol; Secretory vesicle
Involvement in disease
Aberrant activation of HCK by HIV-1 protein Nef enhances HIV-1 replication and contributes to HIV-1 pathogenicity.
Aberrant activation of HCK, e.g. by the BCR-ABL fusion protein, promotes cancer cell proliferation.
PTM
Phosphorylated on several tyrosine residues. Autophosphorylated. Becomes rapidly phosphorylated upon activation of the immunoglobulin receptors FCGR1A and FCGR2A. Phosphorylation by the BCR-ABL fusion protein mediates activation of HCK. Phosphorylation at Tyr-411 increases kinase activity. Phosphorylation at Tyr-522 inhibits kinase activity. Kinase activity is not required for phosphorylation at Tyr-522, suggesting that this site is a target of other kinases.9 Publications
Ubiquitinated by CBL, leading to its degradation via the proteasome.
Isoform 2 palmitoylation at position 2 requires prior myristoylation. Palmitoylation at position 3 is required for caveolar localization of isoform 2.
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For research use only. Not intended for any clinical use.

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