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Mouse Anti-HAS2 Recombinant Antibody (CBFYH-0711) (CBMAB-H1600-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-0711
Antibody Isotype
IgG1
Application
WB, IHC-P, FC, IF

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Antiserum
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Hyaluronan Synthase 2
Introduction
Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS2 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to glycosaminoglycan synthetase (DG42) from Xenopus laevis, and human and murine hyaluronan synthase 1.
Entrez Gene ID
3037
UniProt ID
Q92819
Alternative Names
Hyaluronan Synthase 2; Hyaluronic Acid Synthase 2; Hyaluronate Synthase 2; HA Synthase 2; EC 2.4.1.212
Function
Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction and it is particularly responsible for the synthesis of high molecular mass hyaluronan. Required for the transition of endocardial cushion cells into mesenchymal cells, a process crucial for heart development. May also play a role in vasculogenesis. High molecular mass hyaluronan also play a role in early contact inhibition a process which stops cell growth when cells come into contact with each other or the extracellular matrix (By similarity).
Biological Process
Atrioventricular canal development Source: UniProtKB
Bone morphogenesis Source: Ensembl
Cellular response to fluid shear stress Source: BHF-UCL
Cellular response to interleukin-1 Source: BHF-UCL
Cellular response to platelet-derived growth factor stimulus Source: UniProtKB
Cellular response to tumor necrosis factor Source: BHF-UCL
Endocardial cushion to mesenchymal transition Source: UniProtKB
Estrous cycle Source: Ensembl
Extracellular matrix assembly Source: UniProtKB
Extracellular polysaccharide biosynthetic process Source: UniProtKB
Hyaluronan biosynthetic process Source: UniProtKB
Kidney development Source: UniProtKB
Positive regulation of cell migration Source: BHF-UCL
Positive regulation of cell population proliferation Source: BHF-UCL
Positive regulation of hyaluronan biosynthetic process Source: Ensembl
Positive regulation of keratinocyte migration Source: Ensembl
Positive regulation of keratinocyte proliferation Source: Ensembl
Positive regulation of monocyte aggregation Source: BHF-UCL
Positive regulation of smooth muscle cell migration Source: Ensembl
Positive regulation of substrate adhesion-dependent cell spreading Source: Ensembl
Positive regulation of urine volume Source: UniProtKB
Regulation of extracellular matrix assembly Source: Ensembl
Renal water absorption Source: UniProtKB
Vasculogenesis Source: UniProtKB
Cellular Location
Membrane
Involvement in disease
A chromosomal aberration involving HAS2 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. 8q12.1 to 8q24.1 intrachromosomal rearrangement with PLAG1.
Topology
Cytoplasmic: 1-11
Helical: 12-32
Extracellular: 33-45
Helical: 46-66
Cytoplasmic: 67-374
Helical: 375-395
Extracellular: 396-402
Helical: 403-423
Cytoplasmic: 424-429
Helical: 430-450
Extracellular: 451-475
Helical: 476-496
Cytoplasmic: 497-510
Helical: 511-531
Extracellular: 532-552
More Infomation

Kim, S. M., Song, G. Y., Shim, A., Lee, J. H., Eom, C. B., Liu, C., ... & Seki, E. (2022). Hyaluronan synthase 2, a target of miR-200c, promotes carbon tetrachloride-induced acute and chronic liver inflammation via regulation of CCL3 and CCL4. Experimental & molecular medicine, 54(6), 739-752.

Sheng, Y., Cao, M., Liu, Y., He, Y., Zhang, G., Du, Y., ... & Yang, C. (2021). Hyaluronan synthase 2 (HAS2) regulates cell phenotype and invadopodia formation in luminal-like breast cancer cells. Molecular and Cellular Biochemistry, 476(9), 3383-3391.

Caon, I., Bartolini, B., Moretto, P., Parnigoni, A., Caravà, E., Vitale, D. L., ... & Vigetti, D. (2020). Sirtuin 1 reduces hyaluronan synthase 2 expression by inhibiting nuclear translocation of NF-κB and expression of the long-noncoding RNA HAS2–AS1. Journal of Biological Chemistry, 295(11), 3485-3496.

Li, H., Guo, H., Lei, C., Liu, L., Xu, L., Feng, Y., ... & Long, X. (2019). Nanotherapy in joints: increasing endogenous hyaluronan production by delivering hyaluronan synthase 2. Advanced Materials, 31(46), 1904535.

de Mera, R. M. F., Arasu, U. T., Kärnä, R., Oikari, S., Rilla, K., Vigetti, D., ... & Deen, A. J. (2019). Effects of mutations in the post-translational modification sites on the trafficking of hyaluronan synthase 2 (HAS2). Matrix Biology, 80, 85-103.

Ishizuka, S., Tsuchiya, S., Ohashi, Y., Terabe, K., Askew, E. B., Ishizuka, N., ... & Knudson, W. (2019). Hyaluronan synthase 2 (HAS2) overexpression diminishes the procatabolic activity of chondrocytes by a mechanism independent of extracellular hyaluronan. Journal of Biological Chemistry, 294(37), 13562-13579.

Yang, Y. M., Noureddin, M., Liu, C., Ohashi, K., Kim, S. Y., Ramnath, D., ... & Seki, E. (2019). Hyaluronan synthase 2–mediated hyaluronan production mediates Notch1 activation and liver fibrosis. Science translational medicine, 11(496), eaat9284.

Rauhala, L., Jokela, T., Kärnä, R., Bart, G., Takabe, P., Oikari, S., ... & Tammi, R. H. (2018). Extracellular ATP activates hyaluronan synthase 2 (HAS2) in epidermal keratinocytes via P2Y2, Ca2+ signaling, and MAPK pathways. Biochemical Journal, 475(10), 1755-1772.

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For research use only. Not intended for any clinical use.

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