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Mouse Anti-HAND1 Antibody (2A9) (CBMAB-0435-YC)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
2A9
Antibody Isotype
IgG2a
Application
IP, MA

Basic Information

Immunogen
Recombinant peptide
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
heart and neural crest derivatives expressed 1
Introduction
HAND1 (heart and neural crest derivatives expressed 1) belongs to the basic helix-loop-helix family of transcription factors and is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. HAND1 functions in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that HAND1 may be required for early trophoblast differentiation.
Entrez Gene ID
9421
UniProt ID
O96004
Alternative Names
bHLHa27; eHand; Hxt; Thing1
Function
Transcription factor that plays an essential role in both trophoblast giant cell differentiation and in cardiac morphogenesis (By similarity).

Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box) (By similarity).

Acts as a transcriptional repressor of SOX15 (By similarity).

In the adult, could be required for ongoing expression of cardiac-specific genes (PubMed:9931445).
Biological Process
Angiogenesis Source: BHF-UCL
Blastocyst development Source: BHF-UCL
Cardiac left ventricle formation Source: BHF-UCL
Cardiac right ventricle formation Source: BHF-UCL
Cardiac septum morphogenesis Source: BHF-UCL
Cartilage morphogenesis Source: Ensembl
Developmental process Source: GO_Central
Embryonic heart tube development Source: BHF-UCL
Embryonic heart tube formation Source: Ensembl
Heart development Source: BHF-UCL
Heart looping Source: BHF-UCL
Mesenchyme development Source: Ensembl
Mesoderm formation Source: Ensembl
Negative regulation of DNA-binding transcription factor activity Source: BHF-UCL
Negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding Source: BHF-UCL
Negative regulation of transcription, DNA-templated Source: BHF-UCL
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Odontogenesis of dentin-containing tooth Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Regulation of transcription by RNA polymerase II Source: GO_Central
Trophectodermal cell differentiation Source: BHF-UCL
Trophoblast giant cell differentiation Source: UniProtKB
Ventricular cardiac muscle tissue morphogenesis Source: BHF-UCL
Cellular Location
Nucleoplasm; Nucleolus. Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity. Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity (By similarity).
PTM
Phosphorylation by PLK4 disrupts the interaction with MDFIC and leads to translocation into the nucleoplasm, allowing dimerization and transcription factor activity.
More Infomation

Kuang, Y., He, Z., Li, L., Wang, C., Cheng, X., Shi, Q., ... & Hu, X. (2023). The developmental regulator HAND1 inhibits gastric carcinogenesis through enhancing ER stress apoptosis via targeting CHOP and BAK which is augmented by cisplatin. International Journal of Biological Sciences, 19(1), 120.

Liu, C., Li, Y., Zhang, L., Zhang, P., Yu, N., Liu, X., ... & Hou, S. (2022). MiRNA-196-5p promotes proliferation and migration in cholangiocarcinoma via HAND1/Wnt/β-catenin signaling pathway. Journal of Oncology, 2022.

Zhu, H., Ren, Q., Yan, Z., Xu, S., Luo, J., Wu, X., & Tang, C. (2022). Human HAND1 inhibits the conversion of cholesterol to steroids in trophoblasts. Journal of Genetics and Genomics, 49(4), 350-363.

George, R. M., Guo, S., Firulli, B. A., Rubart, M., & Firulli, A. B. (2022). Neonatal deletion of Hand1 and Hand2 within murine cardiac conduction system reveals a novel role for HAND2 in rhythm homeostasis. Journal of Cardiovascular Development and Disease, 9(7), 214.

Hemming, M. L., Coy, S., Lin, J. R., Andersen, J. L., Przybyl, J., Mazzola, E., ... & Santagata, S. (2021). HAND1 and BARX1 act as transcriptional and anatomic determinants of malignancy in gastrointestinal stromal tumor. Clinical Cancer Research, 27(6), 1706-1719.

Zheng, M., Erhardt, S., Ai, D., & Wang, J. (2021). Bmp Signaling Regulates Hand1 in a Dose-Dependent Manner during Heart Development. International Journal of Molecular Sciences, 22(18), 9835.

Okubo, C., Narita, M., Inagaki, A., Nishikawa, M., Hotta, A., Yamanaka, S., & Yoshida, Y. (2021). Expression dynamics of HAND1/2 in in vitro human cardiomyocyte differentiation. Stem cell reports, 16(8), 1906-1922.

Courtney, J. A., Wilson, R. L., Cnota, J., & Jones, H. N. (2021). Conditional mutation of Hand1 in the mouse placenta disrupts placental vascular development resulting in fetal loss in both early and late pregnancy. International Journal of Molecular Sciences, 22(17), 9532.

Firulli, B. A., George, R. M., Harkin, J., Toolan, K. P., Gao, H., Liu, Y., ... & Firulli, A. B. (2020). HAND1 loss-of-function within the embryonic myocardium reveals survivable congenital cardiac defects and adult heart failure. Cardiovascular research, 116(3), 605-618.

Vincentz, J. W., Firulli, B. A., Toolan, K. P., Arking, D. E., Sotoodehnia, N., Wan, J., ... & Firulli, A. B. (2019). Variation in a left ventricle–specific Hand1 enhancer impairs GATA transcription factor binding and disrupts conduction system development and function. Circulation research, 125(6), 575-589.

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For research use only. Not intended for any clinical use.

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