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Mouse Anti-H2BC1 (AA 2-127) Recombinant Antibody (CBFYH-1164) (CBMAB-H2121-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-1164
Antibody Isotype
IgG1
Application
WB, IHC

Basic Information

Immunogen
Human recombinant protein fragment corresponding to amino acids 2-127 of human HIST1H2BA produced in E.coli
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, PH 7.3, 1% BSA, 50% glycerol
Preservative
0.02% Sodium azide
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 2-127

Target

Full Name
H2B clustered histone 1
Introduction
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element.
Entrez Gene ID
255626
UniProt ID
Q96A08
Alternative Names
Histone Cluster 1 H2B Family Member A; H2B Histone Family, Member U, (Testis-Specific); Testis-Specific Histone H2B; Histone Cluster 1, H2ba; Histone H2B, Testis; Histone 1, H2ba; TSH2B.1; HTSH2B
Function
Variant histone specifically required to direct the transformation of dissociating nucleosomes to protamine in male germ cells (By similarity).

Entirely replaces classical histone H2B prior nucleosome to protamine transition and probably acts as a nucleosome dissociating factor that creates a more dynamic chromatin, facilitating the large-scale exchange of histones (By similarity).

Core component of nucleosome (By similarity).

Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template (By similarity).

Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability (By similarity).

DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling (By similarity).

Also found in fat cells, its function and the presence of post-translational modifications specific to such cells are still unclear (PubMed:21249133).
Biological Process
Nucleosome assembly Source: UniProtKB
Nucleosome disassembly Source: UniProtKB
Spermatogenesis, exchange of chromosomal proteins Source: UniProtKB
Cellular Location
Nucleus; Chromosome
PTM
Monoubiquitination at Lys-36 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-122 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons.
Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
Acetylated during spermatogenesis. Acetylated form is most abundant in spermatogonia compared to spermatocytes and round spermatids (By similarity).
Phosphorylated at Thr-117 in spermatogonia, spermatocytes and round spermatids.
Methylated at Lys-118 in spermatogonia, spermatocytes and round spermatids.
Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription.
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For research use only. Not intended for any clinical use.

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