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Mouse Anti-GRHL3 Recombinant Antibody (CBLG1-3207) (CBMAB-G4847-LY)


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Summary

Host Animal
Mouse
Specificity
Mouse, Rat, Human
Clone
CBLG1-3207
Antibody Isotype
IgG
Application
WB

Basic Information

Specificity
Mouse, Rat, Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
grainyhead-like 3 (Drosophila)
Introduction
This gene encodes a member of the grainyhead family of transcription factors. The encoded protein may function as a transcription factor during development, and has been shown to stimulate migration of endothelial cells. Multiple transcript variants encoding distinct isoforms have been identified for this gene.[provided by RefSeq, Aug 2010]
Entrez Gene ID
Human57822
Mouse230824
Rat298555
UniProt ID
HumanQ8TE85
MouseQ5FWH3
RatD4A1E5
Function
Transcription factor playing important roles in primary neurulation and in the differentiation of stratified epithelia of both ectodermal and endodermal origin (By similarity).

Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' acting as an activator and repressor on distinct target genes (PubMed:21081122, PubMed:25347468).

xhibits functional redundancy with GRHL2 in epidermal morphogenetic events and epidermal wound repair (By similarity).

Exhibits functional redundancy with GRHL2 in epidermal morphogenetic events and epidermal wound repair but is essential to form the epidermal barrier with TGM3 as critical direct target gene among others. Despite being dispensable during normal epidermal homeostasis in the adulthood, is again required for barrier repair after immune-mediated epidermal damage, regulates distinct gene batteries in embryonic epidermal differentiation and adult epidermal barrier reformation after injury. Plays unique and cooperative roles with GRHL2 in establishing distinct zones of primary neurulation. Essential for spinal closure, functions cooperatively with GRHL2 in closure 2 (forebrain/midbrain boundary) and posterior neuropore closure (By similarity).

Also required for proper development of the oral periderm (PubMed:24360809).

No genetic interaction with GRHL3, no functional cooperativity due to diverse target gene selectivity (PubMed:21081122).
Biological Process
Central nervous system development Source: Ensembl
Cochlea morphogenesis Source: Ensembl
Ectoderm development Source: Ensembl
Epidermis development Source: UniProtKB
Establishment of planar polarity Source: Ensembl
Establishment of skin barrier Source: Ensembl
Eyelid development in camera-type eye Source: Ensembl
Neural tube closure Source: UniProtKB
Pattern specification process Source: Ensembl
Planar cell polarity pathway involved in neural tube closure Source: Ensembl
Positive regulation of gene expression Source: Ensembl
Positive regulation of GTPase activity Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: HGNC
Regulation of actin cytoskeleton organization Source: MGI
Regulation of transcription by RNA polymerase II Source: GO_Central
Wound healing Source: Ensembl
Cellular Location
Nucleus
Involvement in disease
Van der Woude syndrome 2 (VWS2):
An autosomal dominant developmental disorder characterized by lower lip pits, cleft lip and/or cleft palate.
More Infomation

Huang, H., Liu, J., Li, M., Guo, H., Zhu, J., Zhu, L., ... & Ouyang, H. (2022). Cis-regulatory chromatin loops analysis identifies GRHL3 as a master regulator of surface epithelium commitment. Science Advances, 8(28), eabo5668.

Chen, X., Lloyd, S. M., Kweon, J., Gamalong, G. M., & Bao, X. (2021). Epidermal progenitors suppress GRHL3-mediated differentiation through intronic polyadenylation promoted by CPSF-HNRNPA3 collaboration. Nature communications, 12(1), 448.

Jaffe, E., & Niswander, L. (2021). Loss of Grhl3 is correlated with altered cellular protrusions in the non‐neural ectoderm during neural tube closure. Developmental Dynamics, 250(5), 732-744.

Kashgari, G., Venkatesh, S., Refuerzo, S., Pham, B., Bayat, A., Klein, R. H., ... & Andersen, B. (2021). GRHL3 activates FSCN1 to relax cell-cell adhesions between migrating keratinocytes during wound reepithelialization. JCI insight, 6(17).

Lin, Z., Jin, S., Chen, J., Li, Z., Lin, Z., Tang, L., ... & Andersen, B. (2020). Murine interfollicular epidermal differentiation is gradualistic with GRHL3 controlling progression from stem to transition cell states. Nature communications, 11(1), 5434.

Sargazi, S., Nia, M. H., Sheervalilou, R., Mirinejad, S., Harati-Sadegh, M., Moudi, M., ... & Shakiba, M. (2020). Relationship between single nucleotide polymorphisms of GRHL3 and schizophrenia susceptibility: a preliminary case-control study and bioinformatics analysis. International journal of molecular and cellular medicine, 9(2), 154.

Kousa, Y. A., Zhu, H., Fakhouri, W. D., Lei, Y., Kinoshita, A., Roushangar, R. R., ... & Schutte, B. C. (2019). The TFAP2A–IRF6–GRHL3 genetic pathway is conserved in neurulation. Human molecular genetics, 28(10), 1726-1737.

Goldie, S. J., Cottle, D. L., Tan, F. H., Roslan, S., Srivastava, S., Brady, R., ... & Darido, C. (2018). Loss of GRHL3 leads to TARC/CCL17-mediated keratinocyte proliferation in the epidermis. Cell Death & Disease, 9(11), 1072.

Eshete, M. A., Liu, H., Li, M., Adeyemo, W. L., Gowans, L. J. J., Mossey, P. A., ... & Butali, A. (2018). Loss-of-function GRHL3 variants detected in African patients with isolated cleft palate. Journal of dental research, 97(1), 41-48.

Tian, T., Wang, L., Shen, Y., Zhang, B., Finnell, R. H., & Ren, A. (2018). Hypomethylation of GRHL3 gene is associated with the occurrence of neural tube defects. Epigenomics, 10(7), 891-901.

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For research use only. Not intended for any clinical use.

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