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Rabbit Anti-FOXJ1 Recombinant Antibody (EG1229) (CBMAB-EN1486-LY)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
EG1229
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 ELISA: 1:20000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from internal of human FOXJ1.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
forkhead box J1
Introduction
This gene encodes a member of the forkhead family of transcription factors. Similar genes in zebrafish and mouse have been shown to regulate the transcription of genes that control the production of motile cilia. The mouse ortholog also functions in the determination of left-right asymmetry. Polymorphisms in this gene are associated with systemic lupus erythematosus and allergic rhinitis.
Entrez Gene ID
Human2302
Mouse15223
Rat116557
UniProt ID
HumanQ92949
MouseQ61660
RatQ63247
Alternative Names
HFH4; HFH-4; CILD43; FKHL13
Function
Transcription factor specifically required for the formation of motile cilia (PubMed:31630787).

Acts by activating transcription of genes that mediate assembly of motile cilia, such as CFAP157. Binds the DNA consensus sequences 5'-HWDTGTTTGTTTA-3' or 5'-KTTTGTTGTTKTW-3' (where H is not G, W is A or T, D is not C, and K is G or T). Activates the transcription of a variety of ciliary proteins in the developing brain and lung.
Biological Process
Actin cytoskeleton organization Source: BHF-UCL
Activation of GTPase activity Source: BHF-UCL
Axoneme assembly Source: UniProtKB
Brain development Source: BHF-UCL
Cellular protein localization Source: UniProtKB
Central tolerance induction Source: BHF-UCL
Ciliary basal body organization Source: UniProtKB
Cilium assembly Source: UniProtKB
Determination of left/right symmetry Source: UniProtKB
Epithelium development Source: BHF-UCL
Establishment of apical/basal cell polarity Source: BHF-UCL
Glomerular parietal epithelial cell development Source: BHF-UCL
Heart development Source: Ensembl
Humoral immune response Source: BHF-UCL
Left/right pattern formation Source: Ensembl
Leukocyte migration Source: BHF-UCL
Lung epithelium development Source: BHF-UCL
Metanephric part of ureteric bud development Source: BHF-UCL
Motile cilium assembly Source: Ensembl
Negative regulation of B cell activation Source: BHF-UCL
Negative regulation of germinal center formation Source: BHF-UCL
Negative regulation of humoral immune response mediated by circulating immunoglobulin Source: BHF-UCL
Negative regulation of interleukin-6 production Source: BHF-UCL
Negative regulation of NF-kappaB transcription factor activity Source: BHF-UCL
Negative regulation of T cell differentiation in thymus Source: BHF-UCL
Negative regulation of T cell proliferation Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Pattern specification process Source: BHF-UCL
Positive regulation of central B cell tolerance induction Source: BHF-UCL
Positive regulation of lung ciliated cell differentiation Source: BHF-UCL
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Regulation of transcription by RNA polymerase II Source: GO_Central
Spermatogenesis Source: UniProtKB
Cellular Location
Nucleus
Involvement in disease
Allergic rhinitis (ALRH):
A common disease with complex inheritance characterized by mucosal inflammation caused by allergen exposure.
Ciliary dyskinesia, primary, 43 (CILD43):
A form of primary ciliary dyskinesia, a disorder characterized by abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia. Patients with this disorder also develop significant obstructive hydrocephalus. Other more variable features include infertility and about a 50% chance of situs inversus or other left-right asymmetry defects. CILD43 inheritance is autosomal dominant.
More Infomation

Weir, A., Kang, E. Y., Meagher, N. S., Nelson, G. S., Ghatage, P., Lee, C. H., ... & Ramus, S. J. (2023). Increased FOXJ1 protein expression is associated with improved overall survival in high-grade serous ovarian carcinoma: An Ovarian Tumor Tissue Analysis Consortium Study. British Journal of Cancer, 128(1), 137-147.

Ma, C., Li, S., Yang, F., Cao, W., Liu, H., Feng, T., ... & Zheng, H. (2022). FoxJ1 inhibits African swine fever virus replication and viral S273R protein decreases the expression of FoxJ1 to impair its antiviral effect. Virologica Sinica, 37(3), 445-454.

Koay, T. W., Osterhof, C., Orlando, I. M., Keppner, A., Andre, D., Yousefian, S., ... & Hoogewijs, D. (2021). Androglobin gene expression patterns and FOXJ1-dependent regulation indicate its functional association with ciliogenesis. Journal of Biological Chemistry, 296.

Kim, H., Lee, Y. S., Kim, S. M., Jang, S., Choi, H., Lee, J. W., ... & Kim, V. N. (2021). RNA demethylation by FTO stabilizes the FOXJ1 mRNA for proper motile ciliogenesis. Developmental Cell, 56(8), 1118-1130.

Shapiro, A. J., Kaspy, K., Daniels, M. L. A., Stonebraker, J. R., Nguyen, V. H., Joyal, L., ... & Zariwala, M. A. (2021). Autosomal dominant variants in FOXJ1 causing primary ciliary dyskinesia in two patients with obstructive hydrocephalus. Molecular Genetics & Genomic Medicine, 9(7), e1726.

Beckers, A., Adis, C., Schuster-Gossler, K., Tveriakhina, L., Ott, T., Fuhl, F., ... & Gossler, A. (2020). The FOXJ1 target Cfap206 is required for sperm motility, mucociliary clearance of the airways and brain development. Development, 147(21), dev188052.

Mukherjee, I., Roy, S., & Chakrabarti, S. (2019). Identification of important effector proteins in the FOXJ1 transcriptional network associated with ciliogenesis and ciliary function. Frontiers in Genetics, 10, 23.

Wallmeier, J., Frank, D., Shoemark, A., Nöthe-Menchen, T., Cindric, S., Olbrich, H., ... & Omran, H. (2019). De novo mutations in FOXJ1 result in a motile ciliopathy with hydrocephalus and randomization of left/right body asymmetry. The American Journal of Human Genetics, 105(5), 1030-1039.

Xian, S., Shang, D., Kong, G., & Tian, Y. (2018). FOXJ1 promotes bladder cancer cell growth and regulates Warburg effect. Biochemical and biophysical research communications, 495(1), 988-994.

Li, X., Floriddia, E. M., Toskas, K., Chalfouh, C., Honore, A., Aumont, A., ... & Barnabé-Heider, F. (2018). FoxJ1 regulates spinal cord development and is required for the maintenance of spinal cord stem cell potential. Experimental Cell Research, 368(1), 84-100.

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For research use only. Not intended for any clinical use.

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