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Mouse Anti-FOXA2 Recombinant Antibody (6G9) (CBMAB-A3089-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
6G9
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
FOXA2 (NP_068556, 363 a.a. ~ 457 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Forkhead Box A2
Introduction
This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]
Entrez Gene ID
3170
UniProt ID
Q9Y261
Alternative Names
HNF3B; MGC19807; TCF3B
Function
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity).

In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
Biological Process
Adult locomotory behavior Source: ParkinsonsUK-UCL
Anatomical structure morphogenesis Source: GO_Central
Cell differentiation Source: GO_Central
Cell fate specification Source: ParkinsonsUK-UCL
Chromatin organization Source: UniProtKB-KW
Dopaminergic neuron differentiation Source: ParkinsonsUK-UCL
Endocrine pancreas development Source: BHF-UCL
Negative regulation of detection of glucose Source: BHF-UCL
Negative regulation of DNA-binding transcription factor activity Source: BHF-UCL
Negative regulation of epithelial to mesenchymal transition Source: BHF-UCL
Negative regulation of glucokinase activity Source: BHF-UCL
Negative regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
Positive regulation of cell-cell adhesion mediated by cadherin Source: BHF-UCL
Positive regulation of embryonic development Source: UniProtKB
Positive regulation of gastrulation Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
Primitive streak formation Source: UniProtKB
Regulation of blood coagulation Source: UniProtKB
Regulation of insulin secretion involved in cellular response to glucose stimulus Source: BHF-UCL
Regulation of transcription by RNA polymerase II Source: BHF-UCL
Response to interleukin-6 Source: UniProtKB
Cellular Location
Cytoplasm; Nucleus. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner; in response to insulin signaling via AKT1 is exported from the nucleus.
PTM
Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
More Infomation

Gao, X. Q., Liu, C. Y., Zhang, Y. H., Wang, Y. H., Zhou, L. Y., Li, X. M., ... & Wang, K. (2022). The circRNA CNEACR regulates necroptosis of cardiomyocytes through Foxa2 suppression. Cell Death & Differentiation, 29(3), 527-539.

Kumar, A., Sundaram, K., Teng, Y., Mu, J., Sriwastva, M. K., Zhang, L., ... & Zhang, H. G. (2022). Ginger nanoparticles mediated induction of Foxa2 prevents high-fat diet-induced insulin resistance. Theranostics, 12(3), 1388.

Han, M., Li, F., Zhang, Y., Dai, P., He, J., Li, Y., ... & Gao, D. (2022). FOXA2 drives lineage plasticity and KIT pathway activation in neuroendocrine prostate cancer. Cancer Cell, 40(11), 1306-1323.

Sahoo, S. S., Ramanand, S. G., Gao, Y., Abbas, A., Kumar, A., Cuevas, I. C., ... & Castrillon, D. H. (2022). FOXA2 suppresses endometrial carcinogenesis and epithelial-mesenchymal transition by regulating enhancer activity. The Journal of Clinical Investigation, 132(12).

Liu, X., Hou, S., Xiang, R., Hu, C., Chen, Z., Li, N., ... & Yang, J. (2022). Imipramine activates FAM3A-FOXA2-CPT2 pathway to ameliorate hepatic steatosis. Metabolism, 136, 155292.

Liu, Q., Pang, J., Wang, L. A., Huang, Z., Xu, J., Yang, X., ... & Jiang, J. (2021). Histone demethylase PHF8 drives neuroendocrine prostate cancer progression by epigenetically upregulating FOXA2. The Journal of Pathology, 253(1), 106-118.

Choi, W., Choe, S., & Lau, G. W. (2020). Inactivation of FOXA2 by respiratory bacterial pathogens and dysregulation of pulmonary mucus homeostasis. Frontiers in immunology, 11, 515.

Lee, K., Cho, H., Rickert, R. W., Li, Q. V., Pulecio, J., Leslie, C. S., & Huangfu, D. (2019). FOXA2 is required for enhancer priming during pancreatic differentiation. Cell reports, 28(2), 382-393.

Cernilogar, F. M., Hasenöder, S., Wang, Z., Scheibner, K., Burtscher, I., Sterr, M., ... & Schotta, G. (2019). Pre-marked chromatin and transcription factor co-binding shape the pioneering activity of Foxa2. Nucleic Acids Research, 47(17), 9069-9086.

Zhu, L., An, L., Ran, D., Lizarraga, R., Bondy, C., Zhou, X., ... & Chen, Y. (2019). The club cell marker SCGB1A1 downstream of FOXA2 is reduced in asthma. American journal of respiratory cell and molecular biology, 60(6), 695-704.

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For research use only. Not intended for any clinical use.

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