Mouse Anti-FDX1 Recombinant Antibody (CBXF-2943) (CBMAB-F3650-CQ)

Name: Mouse Anti-FDX1 Recombinant Antibody (CBXF-2943)
SKU: CBMAB-F3650-CQ
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBXF-2943

Antibody Isotype

IgG2b, κ

Application

ELISA, WB

Basic Information

Immunogen

Partial recombinant corresponding to aa85-183 from human FDX1 (NP_004100.1) with GST tag

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

ferredoxin 1

Introduction

This gene encodes a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to mitochondrial cytochrome P450, involved in steroid, vitamin D, and bile acid metabolism. Pseudogenes of this functional gene are found on chromosomes 20 and 21.

Entrez Gene ID

2230

UniProt ID

P10109

Alternative Names

Ferredoxin 1; Adrenal Ferredoxin; Hepatoredoxin; ADX; Adrenodoxin, Mitochondrial; Mitochondrial Adrenodoxin;

Research Area

Essential for the synthesis of various steroid hormones (PubMed:20547883, PubMed:21636783).

Participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis (PubMed:20547883, PubMed:21636783).

Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage (PubMed:20547883, PubMed:21636783).

Does not form a ternary complex with adrenodoxin reductase and CYP11A1 but shuttles between the two enzymes to transfer electrons (By similarity).

Biological Process

Cellular response to cAMP Source: Ensembl
Cellular response to forskolin Source: Ensembl
Cholesterol metabolic process Source: UniProtKB
Hormone biosynthetic process Source: UniProtKB
Steroid biosynthetic process Source: UniProtKB-KW

Cellular Location

Mitochondrion matrix

More Infomation

References

Li, X., Dai, Z., Liu, J., Sun, Z., Li, N., Jiao, G., & Cao, H. (2022). Characterization of the functional effects of ferredoxin 1 as a cuproptosis biomarker in cancer. Frontiers in Genetics, 13.

Yang, L., Zhang, Y., Wang, Y., Jiang, P., Liu, F., & Feng, N. (2022). Ferredoxin 1 is a cuproptosis-key gene responsible for tumor immunity and drug sensitivity: A pan-cancer analysis. Frontiers in Pharmacology, 13.

Schulz, V., Basu, S., Freibert, S. A., Webert, H., Boss, L., Mühlenhoff, U., ... & Lill, R. (2022). Functional spectrum and specificity of mitochondrial ferredoxins FDX1 and FDX2. Nature Chemical Biology, 1-12.

Zhang, W. T., Gong, Y. M., Zhang, C. Y., Pan, J. S., Huang, T., & Li, Y. X. (2022). A Novel Cuprotosis-Related Gene FDX1 Signature for Overall Survival Prediction in Clear Cell Renal Cell Carcinoma Patients. BioMed Research International, 2022.

Huang, X., Wang, T., Ye, J., Feng, H., Zhang, X., Ma, X., ... & Zhang, X. (2022). FDX1 expression predicts favourable prognosis in clear cell renal cell carcinoma identified by bioinformatics and tissue microarray analysis. Frontiers in Genetics, 13, 994741.

Tsvetkov, P., Detappe, A., Cai, K., Keys, H. R., Brune, Z., Ying, W., ... & Lindquist, S. (2018). Inhibition of mitochondrial ferredoxin 1 (FDX1) prevents adaptation to proteotoxic stress. bioRxiv, 288365.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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