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Recombinant Mouse Anti-F8 Antibody (CB2978) (CBMAB-MD1179-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CB2978
Antibody Isotype
IgG1
Application
ELISA, WB

Basic Information

Immunogen
Human Factor VIII heavy chain
Specificity
Human
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
Lyophilized from PBS, pH 7.4.
Preservative
0.01% Sodium Azide
Purity
> 98% Purity determined by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Coagulation Factor VIII
Entrez Gene ID
2157
UniProt ID
P00451
Research Area
Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.
Biological Process
Acute-phase response Source: UniProtKB-KW
Blood coagulation Source: ProtInc
Blood coagulation, intrinsic pathway Source: GO_Central
Cellular Location
Extracellular space
Involvement in disease
Hemophilia A (HEMA):
The disease is caused by variants affecting the gene represented in this entry. Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non-functional; i.e. the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein. A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery.
PTM
Sulfation on Tyr-1699 is essential for binding vWF.
More Infomation

Han, J. P., Song, D. W., Lee, J. H., Lee, G. S., & Yeom, S. C. (2021). Novel severe hemophilia a mouse model with factor VIII intron 22 inversion. Biology, 10(8), 704.

Collins, A. E., Curry, N., Raza‐Burton, S., Bignell, P., Davies, C., Joffe, M., ... & Desborough, M. J. (2021). Factor VIII levels and bleeding according to factor 8 (F8) mutation in pregnant carriers of haemophilia A: a multicentre retrospective cohort study. British journal of haematology, 193(2), 397-400.

Simioni, P., Cagnin, S., Sartorello, F., Sales, G., Pagani, L., Bulato, C., ... & Castoldi, E. (2021). Partial F8 gene duplication (factor VIII Padua) associated with high factor VIII levels and familial thrombophilia. Blood, 137(17), 2383-2393.

Gong, J., Chung, T. H., Zheng, J., Zheng, H., & Chang, L. J. (2021). Transduction of modified factor VIII gene improves lentiviral gene therapy efficacy for hemophilia A. Journal of Biological Chemistry, 297(6).

Mamoor, S. (2021). Differential expression of coagulation factor VIII in cancers of the breast.

Chen, C. Y., Tran, D. M., Cavedon, A., Cai, X., Rajendran, R., Lyle, M. J., ... & Miao, C. H. (2020). Treatment of hemophilia A using factor VIII messenger RNA lipid nanoparticles. Molecular Therapy-Nucleic Acids, 20, 534-544.

Chen, H., Shi, M., Gilam, A., Zheng, Q., Zhang, Y., Afrikanova, I., ... & Chen-Tsai, R. Y. (2019). Hemophilia A ameliorated in mice by CRISPR-based in vivo genome editing of human Factor VIII. Scientific reports, 9(1), 1-15.

Neumeyer, J., Lin, R. Z., Wang, K., Hong, X., Hua, T., Croteau, S. E., ... & Melero-Martin, J. M. (2019). Bioengineering hemophilia A–specific microvascular grafts for delivery of full-length factor VIII into the bloodstream. Blood advances, 3(24), 4166-4176.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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