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Rabbit Anti-EZR Recombinant Antibody (CBFYE-1440) (CBMAB-E2067-FY)


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Summary

Host Animal
Rabbit
Specificity
Human
Clone
CBFYE-1440
Antibody Isotype
IgG
Application
WB, IHC-P

Basic Information

Immunogen
A synthetic peptide corresponding to residues in human Ezrin was used as an immunogen
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
50% Glycerol, 0.05% BSA, pH7.4
Preservative
0.01% Sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ezrin
Introduction
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.
Entrez Gene ID
7430
UniProt ID
P15311
Alternative Names
Ezrin; Villin 2 (Ezrin); Cytovillin 2; VIL2; P81; Epididymis Secretory Protein Li 105
Research Area
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
Biological Process
Actin cytoskeleton reorganization Source: UniProtKB
Actin filament bundle assembly Source: UniProtKB
Astral microtubule organization Source: UniProtKB
Cellular protein-containing complex localization Source: UniProtKB
Cellular response to cAMP Source: UniProtKB
Cortical microtubule organization Source: UniProtKB
Establishment of centrosome localization Source: UniProtKB
Establishment of endothelial barrier Source: UniProtKB
Establishment or maintenance of apical/basal cell polarity Source: Ensembl
Filopodium assembly Source: UniProtKB
Intestinal D-glucose absorption Source: Ensembl
Leukocyte cell-cell adhesion Source: BHF-UCL
Membrane to membrane docking Source: BHF-UCL
Microvillus assembly Source: UniProtKB
Negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
Negative regulation of interleukin-2 production Source: UniProtKB
Negative regulation of p38MAPK cascade Source: UniProtKB
Negative regulation of T cell receptor signaling pathway Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Phosphatidylinositol-mediated signaling Source: UniProtKB
Positive regulation of cellular protein catabolic process Source: UniProtKB
Positive regulation of early endosome to late endosome transport Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Positive regulation of multicellular organism growth Source: Ensembl
Positive regulation of protein localization to early endosome Source: UniProtKB
Positive regulation of protein localization to plasma membrane Source: Ensembl
Positive regulation of protein secretion Source: UniProtKB
Protein kinase A signaling Source: UniProtKB
Protein localization to cell cortex Source: UniProtKB
Protein localization to plasma membrane Source: UniProtKB
Receptor internalization Source: Ensembl
Regulation of cell shape Source: UniProtKB
Regulation of microvillus length Source: Ensembl
Regulation of organelle assembly Source: UniProtKB
Sphingosine-1-phosphate receptor signaling pathway Source: UniProtKB
Terminal web assembly Source: Ensembl
Cellular Location
Cytoskeleton; Apical cell membrane; Microvillus membrane; Ruffle membrane; Cell projection; Cell cortex; Microvillus. Localization to the apical membrane of parietal cells depends on the interaction with PALS1. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein (cytoplasmic side).
PTM
Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity).
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
More Infomation

Lipreri da Silva, J. C., Carvalho, M. F. L., de Miranda, L. B. L., de Almeida, B. O., Lima, K., & Machado-Neto, J. A. (2022). NSC305787, a pharmacological ezrin inhibitor, exhibits antineoplastic activity in pancreatic cancer cells. Investigational New Drugs, 1-10.

da Silva, J. C. L., Carvalho, M. F. L., de Miranda, L. B. L., de Almeida, B. O., Lima, K., & Machado-Neto, J. A. (2022). NSC305787, a pharmacological ezrin inhibitor, exhibits antineoplastic activity in pancreatic cancer cells. Investigational new drugs, 40(4), 728-737.

Lipreri da Silva, J. C., Coelho-Silva, J. L., Lima, K., Vicari, H. P., Lazarini, M., Costa-Lotufo, L. V., ... & Machado-Neto, J. A. (2021). Comprehensive analysis of cytoskeleton regulatory genes identifies ezrin as a prognostic marker and molecular target in acute myeloid leukemia. Cellular Oncology, 44(5), 1105-1117.

Fan, C., Qu, H., Xiong, F., Tang, Y., Tang, T., Zhang, L., ... & Zeng, Z. (2021). CircARHGAP12 promotes nasopharyngeal carcinoma migration and invasion via ezrin-mediated cytoskeletal remodeling. Cancer Letters, 496, 41-56.

Qureshi-Baig, K., Kuhn, D., Viry, E., Pozdeev, V. I., Schmitz, M., Rodriguez, F., ... & Letellier, E. (2020). Hypoxia-induced autophagy drives colorectal cancer initiation and progression by activating the PRKC/PKC-EZR (ezrin) pathway. Autophagy, 16(8), 1436-1452.

Chang, Y. T., Peng, H. Y., Hu, C. M., Huang, S. C., Tien, S. C., & Jeng, Y. M. (2020). Pancreatic cancer-derived small extracellular vesical Ezrin regulates macrophage polarization and promotes metastasis. American Journal of Cancer Research, 10(1), 12.

Yano, K., Okabe, C., Fujii, K., Kato, Y., & Ogihara, T. (2020). Regulation of breast cancer resistance protein and P-glycoprotein by ezrin, radixin and moesin in lung, intestinal and renal cancer cell lines. Journal of Pharmacy and Pharmacology, 72(4), 575-582.

Zhang, R., Zhang, S., Xing, R., & Zhang, Q. (2019). High expression of EZR (ezrin) gene is correlated with the poor overall survival of breast cancer patients. Thoracic cancer, 10(10), 1953-1961.

Ye, D. J., Kwon, Y. J., Baek, H. S., Shin, S., Lee, C., Yun, J. W., ... & Chun, Y. J. (2018). Discovery of ezrin expression as a potential biomarker for chemically induced ocular irritation using human corneal epithelium cell line and a reconstructed human cornea-like epithelium model. Toxicological Sciences, 165(2), 335-346.

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For research use only. Not intended for any clinical use.

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