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Mouse Anti-ELANE Recombinant Antibody (4B11) (CBMAB-A2557-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
4B11
Antibody Isotype
IgG2a, κ
Application
sELISA, ELISA

Basic Information

Immunogen
ELA2 (NP_001963, 168 a.a. ~ 267 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Elastase, Neutrophil Expressed
Introduction
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq]
Entrez Gene ID
1991
UniProt ID
P08246
Alternative Names
GE; HLE; HNE; NE; PMN-E
Research Area
Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022).

Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984).
Biological Process
Acute inflammatory response to antigenic stimulus Source: GO_Central
Biosynthetic process of antibacterial peptides active against Gram-negative bacteria Source: UniProtKB
Cellular calcium ion homeostasis Source: UniProtKB
Defense response to bacterium Source: UniProtKB
Extracellular matrix disassembly Source: Reactome
Leukocyte migration involved in inflammatory response Source: Ensembl
Negative regulation of chemokine production Source: UniProtKB
Negative regulation of chemotaxis Source: UniProtKB
Negative regulation of inflammatory response Source: UniProtKB
Negative regulation of interleukin-8 production Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Neutrophil-mediated killing of fungus Source: Ensembl
Neutrophil-mediated killing of gram-negative bacterium Source: UniProtKB
Phagocytosis Source: GO_Central
Positive regulation of immune response Source: Ensembl
Positive regulation of interleukin-8 production Source: UniProtKB
Positive regulation of leukocyte tethering or rolling Source: Ensembl
Positive regulation of MAP kinase activity Source: UniProtKB
Positive regulation of smooth muscle cell proliferation Source: UniProtKB
Protein catabolic process Source: UniProtKB
Proteolysis Source: UniProtKB
Pyroptosis Source: Reactome
Response to lipopolysaccharide Source: Ensembl
Response to UV Source: UniProtKB
Response to yeast Source: Ensembl
Cellular Location
Phagosome. Localized in phagolysosomes following ingestion of E.coli by neutrophils.
Involvement in disease
Cyclic haematopoiesis (CH):
Autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.
Neutropenia, severe congenital 1, autosomal dominant (SCN1):
A disorder of hematopoiesis characterized by maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
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For research use only. Not intended for any clinical use.

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