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Rabbit Anti-EIF5 Recombinant Antibody (D5G9) (CBMAB-CP0572-LY)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Clone
D5G9
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human eIF5 protein.
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Eukaryotic Translation Initiation Factor 5
Introduction
Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).[supplied by OMIM, May 2010]
Entrez Gene ID
Human1983
Mouse217869
Rat56783
Monkey697538
UniProt ID
HumanP55010
MouseP59325
RatQ07205
Alternative Names
Eukaryotic Translation Initiation Factor 5; EIF-5; EIF-5A;
Research Area
Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).
Biological Process
Activation of GTPase activity Source: Ensembl
Formation of cytoplasmic translation initiation complex Source: GO_Central
Formation of translation preinitiation complex Source: GO_Central
Regulation of translational initiation Source: CACAO
Cellular Location
Cytosol; Plasma membrane; Cytoplasm
More Infomation

Li, Z., Wu, Y., Fu, Y., Chen, X., Zhao, X., Wu, X., ... & Sun, F. (2022). Cyst stem cell lineage eIF5 non-autonomously prevents testicular germ cell tumor formation via eIF1A/eIF2γ-mediated pre-initiation complex. Stem cell research & therapy, 13(1), 1-18.

Paul, E. E., Lin, K. Y., Gamble, N., Tsai, A. W. L., Swan, S. H., Yang, Y., ... & Marintchev, A. (2022). Dynamic interaction network involving the conserved intrinsically disordered regions in human eIF5. Biophysical Chemistry, 281, 106740.

Singh, C. R., Jaiswal, R., Escalante, C. R., & Asano, K. (2022). Label-free protocol to quantify protein affinity using isothermal titration calorimetry and bio-layer interferometry of a human eIF5-mimic protein. STAR protocols, 3(3), 101615.

Marintchev, A., Paul, E. E., Lin, K. Y., Tsai, A. W. L., Yang, Y., Gamble, N., & Doran, M. (2022). Dynamic Interaction Network Involving the Conserved Intrinsically Disordered Regions in Human eIF5. The FASEB Journal, 36.

Cai, S. X., Chen, W. S., Zeng, W., Cheng, X. F., Lin, M. B., & Wang, J. S. (2021). Roles of HDAC2, eIF5, and eIF6 in lung cancer tumorigenesis. Current medical science, 41(4), 764-769.

Zeman, J., Itoh, Y., Kukačka, Z., Rosůlek, M., Kavan, D., Kouba, T., ... & Valášek, L. S. (2019). Binding of eIF3 in complex with eIF5 and eIF1 to the 40S ribosomal subunit is accompanied by dramatic structural changes. Nucleic acids research, 47(15), 8282-8300.

Lin, K. Y., Nag, N., Pestova, T. V., & Marintchev, A. (2018). Human eIF5 and eIF1A compete for binding to eIF5B. Biochemistry, 57(40), 5910-5920.

Llácer, J. L., Hussain, T., Saini, A. K., Nanda, J. S., Kaur, S., Gordiyenko, Y., ... & Ramakrishnan, V. (2018). Translational initiation factor eIF5 replaces eIF1 on the 40S ribosomal subunit to promote start-codon recognition. Elife, 7, e39273.

Ross, J. A., Bressler, K. R., & Thakor, N. (2018). Eukaryotic initiation factor 5B (eIF5B) cooperates with eIF1A and eIF5 to facilitate uORF2-mediated repression of ATF4 translation. International journal of molecular sciences, 19(12), 4032.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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