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Mouse Anti-EIF3A (AA 1-75) Recombinant Antibody (CBFYE-0673) (CBMAB-E1091-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYE-0673
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
EIF3A partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen sequence: MPAYFQRPEN ALKRANEFLE VGKKQPALDV LYDVMKSKKH RTWQKIHEPI MLKYLELCVD LRKSHLAKEG LYQYK
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-75

Target

Full Name
Eukaryotic Translation Initiation Factor 3 Subunit A
Introduction
EIF3A (Eukaryotic Translation Initiation Factor 3 Subunit A) is a Protein Coding gene. Diseases associated with EIF3A include Bardet-Biedl Syndrome. Among its related pathways are Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S and Viral mRNA Translation. Gene Ontology (GO) annotations related to this gene include translation initiation factor activity.
Entrez Gene ID
8661
UniProt ID
Q14152
Alternative Names
Eukaryotic Translation Initiation Factor 3 Subunit A; Eukaryotic Translation Initiation Factor 3, Subunit 10 Theta, 150/170kDa; Eukaryotic Translation Initiation Factor 3 Subunit 10; EIF-3-Theta; EIF3 P167; EIF3 P180; EIF3 P185; EIF3S10; Eukaryotic Translation Initiation Factor 3, Subunit 10 (Theta, 150/170kD); Eukaryotic Translation Initiation Factor 3, Subunit 10 (Theta, 170kD); Eukaryotic Translation Initiation Factor 3, Subunit 10, 170kD; Eukaryotic Translation Initiation Factor 3, Subunit A; Cytoplasmic Protein P167
Research Area
RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773).

The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732).

The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773, PubMed:27462815).

(Microbial infection) Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed:23766293, PubMed:24357634).

(Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426).
Biological Process
Formation of cytoplasmic translation initiation complex Source: UniProtKB
IRES-dependent viral translational initiation Source: UniProtKB
Negative regulation of ERK1 and ERK2 cascade Source: Ensembl
Translational initiation Source: UniProtKB
Translation reinitiation Source: GO_Central
Viral translational termination-reinitiation Source: UniProtKB
Cellular Location
Cytoplasm
PTM
Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
More Infomation

Ma, S., Dong, Z., Huang, Y., Liu, J. Y., & Zhang, J. T. (2022). eIF3a regulation of mTOR signaling and translational control via HuR in cellular response to DNA damage. Oncogene, 41(17), 2431-2443.

Zhao, Y., Zhao, H., Zhang, D., Quan, Q., Ge, Y., Li, L., & Guo, L. (2022). YTHDF3 Facilitates eIF2AK2 and eIF3A Recruitment on mRNAs to Regulate Translational Processes in Oxaliplatin-Resistant Colorectal Cancer. ACS Chemical Biology, 17(7), 1778-1788.

Shu, X. E., Mao, Y., Jia, L., & Qian, S. B. (2022). Dynamic eIF3a O-GlcNAcylation controls translation reinitiation during nutrient stress. Nature Chemical Biology, 18(2), 134-141.

Mei, C., Sun, Z. E., Tan, L. M., Gong, J. P., Li, X., & Liu, Z. Q. (2022). eIF3a‐PPP2R5A‐mediated ATM/ATR dephosphorylation is essential for irinotecan‐induced DNA damage response. Cell proliferation, 55(4), e13208.

Jiang, J. S., Zang, J., Ru, Y., Luo, Y., Song, J. K., Luo, Y., ... & Li, B. (2022). Patient-driven discovery of CCN1 to rescue cutaneous wound healing in diabetes via the intracellular EIF3A/CCN1/ATG7 signaling by nanoparticle-enabled delivery. Biomaterials, 121698.

Chen, J., Liu, J. Y., Dong, Z. Z., Zou, T., Wang, Z., Shen, Y., ... & Yin, J. Y. (2021). The effect of eIF3a on anthracycline-based chemotherapy resistance by regulating DSB DNA repair. Biochemical Pharmacology, 190, 114616.

Miao, B., Wei, C., Qiao, Z., Han, W., Chai, X., Lu, J., ... & Cai, J. (2019). eIF3a mediates HIF1α-dependent glycolytic metabolism in hepatocellular carcinoma cells through translational regulation. American Journal of Cancer Research, 9(5), 1079.

Heo, C. K., Hwang, H. M., Lee, H. J., Kwak, S. S., Yoo, J. S., Yu, D. Y., ... & Cho, E. W. (2019). Serum anti-EIF3A autoantibody as a potential diagnostic marker for hepatocellular carcinoma. Scientific reports, 9(1), 1-13.

Huang, M. S., Yuan, F. Q., Gao, Y., Liu, J. Y., Chen, Y. X., Wang, C. J., ... & Liu, Z. Q. (2019). Circular RNA screening from EIF3a in lung cancer. Cancer Medicine, 8(9), 4159-4168.

Yin, J. Y., Zhang, J. T., Zhang, W., Zhou, H. H., & Liu, Z. Q. (2018). eIF3a: a new anticancer drug target in the eIF family. Cancer letters, 412, 81-87.

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For research use only. Not intended for any clinical use.

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