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Mouse Anti-EEF2K Recombinant Antibody (3F6) (CBMAB-0514-CN)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
3F6
Antibody Isotype
IgG2a
Application
ELISA, MA

Basic Information

Immunogen
Recombinant protein of EEF2K
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
EEF2K
Introduction
Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.
Entrez Gene ID
29904
UniProt ID
O00418
Alternative Names
Eukaryotic Elongation Factor 2 Kinase; Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase; EEF-2 Kinase; EC 2.7.11.20; EEF-2K; Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor-2 Kinase; Calmodulin-Dependent Protein Kinase III; Eukaroytic Elongation Factor 2 Kinase; Eukaryotic Elongation Factor-2 Kinase; Elongation Factor-2 Kinase; EC 2.7.11; HSU93850
Research Area
Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
Biological Process
Cellular response to anoxia Source: Ensembl
Cellular response to brain-derived neurotrophic factor stimulus Source: Ensembl
Cellular response to calcium ion Source: Ensembl
Cellular response to cAMP Source: Ensembl
Cellular response to insulin stimulus Source: Ensembl
Negative regulation of apoptotic process Source: Ensembl
Positive regulation of dendritic spine morphogenesis Source: Ensembl
Positive regulation of endocytosis Source: Ensembl
Positive regulation of synapse assembly Source: Ensembl
Protein autophosphorylation Source: UniProtKB
Regulation of protein autophosphorylation Source: Ensembl
Response to ischemia Source: Ensembl
Response to prolactin Source: Ensembl
Translational elongation Source: ProtInc
Cellular Location
Cytosol; Cytoplasm; Dendritic spine; Postsynaptic density
PTM
Autophosphorylated at multiple residues, Thr-348 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-398 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 at Ser-366 instead decrease EEF2K activity and promote protein synthesis.
More Infomation

Beretta, S., Gritti, L., Ponzoni, L., Scalmani, P., Mantegazza, M., Sala, M., ... & Sala, C. (2022). Rescuing epileptic and behavioral alterations in a Dravet syndrome mouse model by inhibiting eukaryotic elongation factor 2 kinase (eEF2K). Molecular autism, 13(1), 1-22.

Onder, F. C., Siyah, P., Durdagi, S., Ay, M., & Ozpolat, B. (2022). Novel Etodolac Derivatives as Eukaryotic Elongation Factor 2 Kinase (eEF2K) Inhibitors for Targeted Cancer Therapy. RSC Medicinal Chemistry.

Ma, T. (2021). Roles of eukaryotic elongation factor 2 kinase (eEF2K) in neuronal plasticity, cognition, and Alzheimer disease. Journal of Neurochemistry.

Zhu, S., Liao, M., Tan, H., Zhu, L., Chen, Y., He, G., & Liu, B. (2021). Inhibiting Eukaryotic Elongation Factor 2 Kinase: An Update on Pharmacological Small-Molecule Compounds in Cancer. Journal of Medicinal Chemistry, 64(13), 8870-8883.

Zhang, B., Zou, J., Zhang, Q., Wang, Z., Wang, N., He, S., ... & Naman, C. B. (2021). Progress in the development of eukaryotic elongation factor 2 kinase (eEF2K) natural product and synthetic small molecule inhibitors for cancer chemotherapy. International Journal of Molecular Sciences, 22(5), 2408.

Karakas, D., & Ozpolat, B. (2020). Eukaryotic elongation factor-2 kinase (eEF2K) signaling in tumor and microenvironment as a novel molecular target. Journal of Molecular Medicine, 98(6), 775-787.

Zhou, Y., Li, Y., Xu, S., Lu, J., Zhu, Z., Chen, S., ... & Shen, K. (2020). Eukaryotic elongation factor 2 kinase promotes angiogenesis in hepatocellular carcinoma via PI3K/Akt and STAT3. International journal of cancer, 146(5), 1383-1395.

Ng, T. H., Sham, K. W., Xie, C. M., Ng, S. S., To, K. F., Tong, J. H., ... & Cheng, C. H. (2019). Eukaryotic elongation factor-2 kinase expression is an independent prognostic factor in colorectal cancer. BMC cancer, 19(1), 1-9.

Jan, A., Jansonius, B., Delaidelli, A., An, Y. A., Ferreira, N., Smits, L. M., ... & Sorensen, P. H. (2018). Activity of translation regulator eukaryotic elongation factor-2 kinase is increased in Parkinson disease brain and its inhibition reduces alpha synuclein toxicity. Acta neuropathologica communications, 6(1), 1-17.

Pan, Z., Chen, Y., Liu, J., Jiang, Q., Yang, S., Guo, L., & He, G. (2018). Design, synthesis, and biological evaluation of polo-like kinase 1/eukaryotic elongation factor 2 kinase (PLK1/EEF2K) dual inhibitors for regulating breast cancer cells apoptosis and autophagy. European journal of medicinal chemistry, 144, 517-528.

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For research use only. Not intended for any clinical use.

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