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Recombinant Rabbit Anti-DUSP6 Antibody (CBAb089) (CBMAB-Ab089-LY)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
CBAb089
Antibody Isotype
IgG
Application
WB 1:500-1:2000; IHC 1:50-1:200; IF 1:50-1:200; IP 1:20-1:50

Basic Information

Immunogen
Recombinant protein of human DUSP6
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Dual Specificity Phosphatase 6
Introduction
The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Mutations in this gene have been associated with congenital hypogonadotropic hypogonadism. Alternatively spliced transcript variants have been found for this gene.
Entrez Gene ID
Human1848
Mouse67603
Rat116663
UniProt ID
HumanQ16828
MouseQ9DBB1
RatQ64346
Alternative Names
DUSP6; HH19; MKP3; PYST1; dual specificity phosphatase 6
Research Area
Inactivates MAP kinases. Has a specificity for the ERK family (PubMed:9858808).

Plays an important role in alleviating chronic postoperative pain. Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia (By similarity).

Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain (By similarity).

Promotes cell differentiation by regulating MAPK1/MAPK3 activity and regulating the expression of AP1 transcription factors (PubMed:29043977).
Biological Process
Activation of MAPK activity Source: Reactome
Cell differentiation Source: Ensembl
Inactivation of MAPK activity Source: UniProtKB
MAPK cascade Source: Reactome
Negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
Peptidyl-threonine dephosphorylation Source: GO_Central
Peptidyl-tyrosine dephosphorylation Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Protein dephosphorylation Source: GO_Central
Regulation of heart growth Source: GO_Central
Response to drug Source: Ensembl
Response to growth factor Source: Ensembl
Response to nitrosative stress Source: UniProtKB
Response to organic cyclic compound Source: Ensembl
Cellular Location
Cytoplasm
Involvement in disease
Hypogonadotropic hypogonadism 19 with or without anosmia (HH19):
The disease is caused by variants affecting distinct genetic loci, including the gene represented in this entry. Some patients carrying mutations in DUSP6 also have a heterozygous mutation in another HH-associated gene including FGFR1 and SPRY4 (PubMed:23643382).
A disorder characterized by absent or incomplete sexual maturation by the age of 18 years, in conjunction with low levels of circulating gonadotropins and testosterone and no other abnormalities of the hypothalamic-pituitary axis. In some cases, it is associated with non-reproductive phenotypes, such as anosmia, cleft palate, and sensorineural hearing loss. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In the presence of anosmia, idiopathic hypogonadotropic hypogonadism is referred to as Kallmann syndrome, whereas in the presence of a normal sense of smell, it has been termed normosmic idiopathic hypogonadotropic hypogonadism (nIHH).
More Infomation

Mao, Z., Gao, M., Zhao, X., Li, L., & Peng, J. (2022). Neuroprotective Effect of Dioscin against Parkinson’s Disease via Adjusting Dual-Specificity Phosphatase 6 (DUSP6)-Mediated Oxidative Stress. Molecules, 27(10), 3151.

Relav, L., Estienne, A., & Price, C. A. (2021). Dual-specificity phosphatase 6 (DUSP6) mRNA and protein abundance is regulated by fibroblast growth factor 2 in sheep granulosa cells and inhibits c-Jun N-terminal kinase (MAPK8) phosphorylation. Molecular and Cellular Endocrinology, 531, 111297.

Zuchegna, C., Di Zazzo, E., Moncharmont, B., & Messina, S. (2020). Dual-specificity phosphatase (DUSP6) in human glioblastoma: epithelial-to-mesenchymal transition (EMT) involvement. BMC research notes, 13(1), 1-8.

Kato, M., Onoyama, I., Yoshida, S., Cui, L., Kawamura, K., Kodama, K., ... & Kato, K. (2020). Dual‐specificity phosphatase 6 plays a critical role in the maintenance of a cancer stem‐like cell phenotype in human endometrial cancer. International journal of cancer, 147(7), 1987-1999.

Ramkissoon, A., Chaney, K. E., Milewski, D., Williams, K. B., Williams, R. L., Choi, K., ... & Ratner, N. (2019). Targeted inhibition of the dual specificity phosphatases DUSP1 and DUSP6 suppress MPNST growth via JNK. Clinical Cancer Research, 25(13), 4117-4127.

Beaudry, K., Langlois, M. J., Montagne, A., Cagnol, S., Carrier, J. C., & Rivard, N. (2019). Dual‐specificity phosphatase 6 deletion protects the colonic epithelium against inflammation and promotes both proliferation and tumorigenesis. Journal of cellular physiology, 234(5), 6731-6745.

Wu, G., Tan, J., Li, J., Sun, X., Du, L., & Tao, S. (2019). miRNA‐145‐5p induces apoptosis after ischemia‐reperfusion by targeting dual specificity phosphatase 6. Journal of Cellular Physiology, 234(9), 16281-16289.

Ahmad, M. K., Abdollah, N. A., Shafie, N. H., Yusof, N. M., & Razak, S. R. A. (2018). Dual-specificity phosphatase 6 (DUSP6): a review of its molecular characteristics and clinical relevance in cancer. Cancer biology & medicine, 15(1), 14.

Hsu, S. F., Lee, Y. B., Lee, Y. C., Chung, A. L., Apaya, M. K., Shyur, L. F., ... & Meng, T. C. (2018). Dual specificity phosphatase DUSP 6 promotes endothelial inflammation through inducible expression of ICAM‐1. The FEBS Journal, 285(9), 1593-1610.

Maurer, J. M., & Sagerström, C. G. (2018). A parental requirement for dual-specificity phosphatase 6 in zebrafish. BMC developmental biology, 18(1), 1-16.

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For research use only. Not intended for any clinical use.

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