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Mouse Anti-DUSP5 Recombinant Antibody (8C122) (CBMAB-D1909-YC)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
8C122
Antibody Isotype
IgG3, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 362-383 at the C-terminus of human DUSP5.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG3, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
dual specificity phosphatase 5
Introduction
DUSP5 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, is expressed in a variety of tissues with the highest levels in pancreas and brain, and is localized in the nucleus.
Entrez Gene ID
1847
UniProt ID
Q16690
Alternative Names
Dual Specificity Phosphatase 5; Dual Specificity Protein Phosphatase HVH3; Serine/Threonine Specific Protein Phosphatase; Dual Specificity Protein Phosphatase 5; VH1-Like Phosphatase 3; EC 3.1.3.16;
Research Area
Dual specificity protein phosphatase; active with phosphotyrosine, phosphoserine and phosphothreonine residues. The highest relative activity is toward ERK1.
Biological Process
Activation of MAPK activity Source: Reactome
Dephosphorylation Source: UniProtKB
Endoderm formation Source: GO_Central
Inactivation of MAPK activity Source: GO_Central
MAPK cascade Source: Reactome
Peptidyl-threonine dephosphorylation Source: UniProtKB
Peptidyl-tyrosine dephosphorylation Source: UniProtKB
Protein dephosphorylation Source: GO_Central
Cellular Location
Nucleus
More Infomation

Hua, C., Zheng, Q., Zhu, J., Chen, S., Song, Y., van der Veen, S., & Cheng, H. (2022). Human Papillomavirus Type 16 Early Protein E7 Activates Autophagy through Inhibition of Dual-Specificity Phosphatase 5. Oxidative medicine and cellular longevity, 2022.

Kidger, A. M., Saville, M. K., Rushworth, L. K., Davidson, J., Stellzig, J., Ono, M., ... & Keyse, S. M. (2022). Suppression of mutant Kirsten-RAS (KRASG12D)-driven pancreatic carcinogenesis by dual-specificity MAP kinase phosphatases 5 and 6. Oncogene, 41(20), 2811-2823.

Tan, L., Bogush, N., Naqvi, E., Calvert, J. W., Graham, R. M., Taylor, W. R., ... & Husain, A. (2021). Thyroid hormone plus dual-specificity phosphatase-5 siRNA increases the number of cardiac muscle cells and improves left ventricular contractile function in chronic doxorubicin-injured hearts. Theranostics, 11(10), 4790.

Luo, J., Xue, D., Song, F., Liu, X., Li, W., & Wang, Y. (2020). DUSP5 (dual-specificity protein phosphatase 5) suppresses BCG-induced autophagy via ERK 1/2 signaling pathway. Molecular Immunology, 126, 101-109.

Zhang, H., Zhang, C., Liu, Y., Gao, W., Wang, S., Fang, X., ... & Fan, F. (2020). Influence of dual‐specificity protein phosphatase 5 on mechanical properties of rat cerebral and renal arterioles. Physiological Reports, 8(2), e14345.

Zhang, C., He, X., Murphy, S. R., Zhang, H., Wang, S., Ge, Y., ... & Fan, F. (2019). Knockout of dual-specificity protein phosphatase 5 protects against hypertension-induced renal injury. Journal of Pharmacology and Experimental Therapeutics, 370(2), 206-217.

Wang, L., Hu, J., Qiu, D., Gao, H., Zhao, W., Huang, Y., ... & Chen, Y. (2019). Dual-specificity phosphatase 5 suppresses ovarian cancer progression by inhibiting IL-33 signaling. American Journal of Translational Research, 11(2), 844.

Alleboina, S., Ayalew, D., Peravali, R., Chen, L., Wong, T., & Dokun, A. O. (2019). Dual specificity phosphatase 5 regulates perfusion recovery in experimental peripheral artery disease. Vascular Medicine, 24(5), 395-404.

Gupta, A., Brahmbhatt, J., Syrlybaeva, R., Bodnar, C., Bodnar, N., Bongard, R., ... & Talipov, M. R. (2019). Role of conserved histidine and serine in the HCXXXXXRS motif of human dual-specificity phosphatase 5. Journal of Chemical Information and Modeling, 59(4), 1563-1574.

Zhang, C., He, X., Wang, S., Roman, R. J., Booz, G., & Fan, F. (2018). Knockout of Dual-Specificity Protein Phosphatase 5 Protects Against Hypertension Induced Chronic Kidney Disease. Hypertension, 72(Suppl_1), A056-A056.

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For research use only. Not intended for any clinical use.

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