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Mouse Anti-DSCAM Monoclonal Antibody (MM0718-42L19) (CBMAB-1076-YC)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
MM0718-42L19
Antibody Isotype
IgG2
Application
ICC, IF, WB

Basic Information

Immunogen
Recombinant fragment corresponding to Human Dscam aa 18-1594. Sequence: EDLHSSLYFVNASLQEVVFASTTGTLVPCPAAGIPPVTLRWYLATGEEIYDVPGIRHVHPNGTLQIFPFPPSSFSTLIHDNTYYCTAENPSGKIRSQDVHIKAVLREPYTVRVEDQKTMRGNVAVFKCIIPSSVEAYITVVSWEKDTVSLVSGSRFLITSTGALYIKDVQNEDGLYNYRCITRHRYTGETRQSNSARLFVSDPANSAPSILDGFDHRKAMAGQRVELPCKALGHPEPDYRWLKDNMPLELSGRFQKTVTGLLIENIRPSDSGSYVCEVSNRYGTAKVIGRLYVKQPLKATISPRKVKSSVGSQVSLSCSVTGTEDQELSWYRNGEILNPGKNVRITGINHENLIMDHMVKSDGGAYQCFVRKDKLSAQDYVQVVLEDGTPKIISAFSEKVVSPAEPVSLMCNVKGTPLPTITWTLDDDPILKGGSHRISQMITSEGNVVSYLNISSSQVRDGGVYRCTANNSAGVVLYQARINVRGPASIRPMKNITAIAGRDTYIHCRVIGYPYYSIKWYKNSNLLPFNHRQVAFENNGTLKLSDVQKEVDEGEYTCNVLVQPQLSTSQSVHVTVKVPPFIQPFEFPRFSIGQRVFIPCVVVSGDLPITITWQKDGRPIPGSLGVTIDNIDFTSSLRISNLSLMHNGNYTCIARNEAAAVEHQSQLIVRVPPKFVVQPRDQDGIYGKAVILNCSAEGYPVPTIVWKFSKGAGVPQFQPIALNGRIQVLSNGSLLIKHVVEEDSGYYLCKVSNDVGADVSKSMYLTVKIPAMITSYPNTTLATQGQKKEMSCTAHGEKPIIVRWEKEDRIINPEMARYLVSTKEVGEEVISTLQILPTVREDSGFFSCHAINSYGEDRGIIQLTVQEPPDPPEIEIKDVKARTITLRWTMGFDGNSPITGYDIECKNKSDSWDSAQRTKDVSPQLNSATIIDIHPSSTYSIRMYAKNRIGKSEPSNELTITADEAAPDGPPQEVHLEPISSQSIRVTWKAPKKHLQNGIIRGYQIGYREYSTGGNFQFNIISVDTSGDSEVYTLDNLNKFTQYGLVVQACNRAGTGPSSQEIITTTLEDVPSYPPENVQAIATSPESISISWSTLSKEALNGILQGFRVIYWANLMDGELGEIKNITTTQPSLELDGLEKYTNYSIQVLAFTRAGDGVRSEQIFTRTKEDVPGPPAGVKAAAASASMVFVSWLPPLKLNGIIRKYTVFCSHPYPTVISEFEASPDSFSYRIPNLSRNRQYSVWVVAVTSAGRGNSSEIITVEPLAKAPARILTFSGTVTTPWMKDIVLPCKAVGDPSPAVKWMKDSNGTPSLVTIDGRRSIFSNGSFIIRTVKAEDSGYYSCIANNNWGSDEIILNLQVQVPPDQPRLTVSKTTSSSITLSWLPGDNGGSSIRGYILQYSEDNSEQWGSFPISPSERSYRLENLKCGTWYKFTLTAQNGVGPGRISEIIEAKTLGKEPQFSKEQELFASINTTRVRLNLIGWNDGGCPITSFTLEYRPFGTTVWTTAQRTSLSKSYILYDLQEATWYELQMRVCNSAGCAEKQANFATLNYDGSTIPPLIKSVVQNEEGLTTNEGLK
Specificity
Human
Antibody Isotype
IgG2
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DS Cell Adhesion Molecule
Introduction
DSCAM is a candidate for Down syndrome and congenital heart disease (DSCHD). DSCAM is a member of the immunoglobulin superfamily of cell adhesion molecules (Ig-CAMs), and is involved in human central and peripheral nervous system development.
Entrez Gene ID
1826
UniProt ID
O60469
Alternative Names
CHD2; CHD2-42; CHD2-52
Research Area
Cell adhesion molecule that plays a role in neuronal self-avoidance. Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Mediates within retinal amacrine and ganglion cell subtypes both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies (PubMed:10925149).

Receptor for netrin required for axon guidance independently of and in collaboration with the receptor DCC. Might also collaborate with UNC5C in NTN1-mediated axon repulsion independently of DCC (By similarity).

In spinal cord development plays a role in guiding commissural axons projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding (PubMed:18585357, PubMed:19196994).

Enhances netrin-induced phosphorylation of PAK1 and FYN (PubMed:15169762).

Mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38 (PubMed:18585357, PubMed:19196994).

Adhesion molecule that promotes lamina-specific synaptic connections in the retina: expressed in specific subsets of interneurons and retinal ganglion cells (RGCs) and promotes synaptic connectivity via homophilic interactions (By similarity).
Biological Process
Axon guidance Source: UniProtKB
Camera-type eye photoreceptor cell differentiation Source: UniProtKB
Cell adhesion Source: ProtInc
Dendrite morphogenesis Source: Ensembl
Dendrite self-avoidance Source: UniProtKB
Homophilic cell adhesion via plasma membrane adhesion molecules Source: UniProtKB
Locomotory behavior Source: Ensembl
Negative regulation of cell adhesion Source: UniProtKB
Nervous system development Source: ProtInc
Positive regulation of axon extension involved in axon guidance Source: UniProtKB
Positive regulation of phosphorylation Source: UniProtKB
Post-embryonic retina morphogenesis in camera-type eye Source: Ensembl
Retina layer formation Source: UniProtKB
Synapse assembly Source: UniProtKB
Cellular Location
Isoform Short: Secreted
Isoform Long: Cell membrane; Axon; Dendrite; Growth cone; Synapse. Localized in the soma, cell membrane, axon and growth cone of dissociated commissural axons.
Topology
Extracellular: 18-1595
Helical: 1596-1616
Cytoplasmic: 1617-2012
PTM
Phosphorylated at tyrosine residues. Phosphorylation is enhanced by NTN1.
More Infomation

Mitsogiannis, M. D., Pancho, A., Aerts, T., Sachse, S. M., Vanlaer, R., Noterdaeme, L., ... & Seuntjens, E. (2021). Subtle Roles of Down Syndrome Cell Adhesion Molecules in Embryonic Forebrain Development and Neuronal Migration. Frontiers in cell and developmental biology, 1859.

Tang, X. Y., Xu, L., Wang, J., Hong, Y., Wang, Y., Zhu, Q., ... & Liu, Y. (2021). DSCAM/PAK1 pathway suppression reverses neurogenesis deficits in iPSC-derived cerebral organoids from patients with Down syndrome. The Journal of clinical investigation, 131(12).

Chen, H., & Cai, K. (2020). DSCAM-AS1 mediates pro-hypertrophy role of GRK2 in cardiac hypertrophy aggravation via absorbing miR-188-5p. In Vitro Cellular & Developmental Biology-Animal, 56(4), 286-295.

Ng, T. H., Kumar, R., Apitanyasai, K., He, S. T., Chiu, S. P., & Wang, H. C. (2019). Selective expression of a “correct cloud” of Dscam in crayfish survivors after second exposure to the same pathogen. Fish & Shellfish Immunology, 92, 430-437.

Li, D., Wan, Z., Li, X., Duan, M., Yang, L., Ruan, Z., ... & Li, W. (2019). Alternatively spliced down syndrome cell adhesion molecule (Dscam) controls innate immunity in crab. Journal of Biological Chemistry, 294(44), 16440-16450.

Li, X. J., Yang, L., Li, D., Zhu, Y. T., Wang, Q., & Li, W. W. (2018). Pathogen-specific binding soluble down syndrome cell adhesion molecule (Dscam) regulates phagocytosis via membrane-bound Dscam in crab. Frontiers in Immunology, 9, 801.

Lowe, S. A., Hodge, J. J., & Usowicz, M. M. (2018). A third copy of the Down syndrome cell adhesion molecule (Dscam) causes synaptic and locomotor dysfunction in Drosophila. Neurobiology of Disease, 110, 93-101.

Stachowicz, K. (2018). The role of DSCAM in the regulation of synaptic plasticity: Possible involvement in neuropsychiatric disorders. Acta Neurobiol. Exp, 78(3), 210-219.

Montesinos, M. L. (2017). Local translation of the Down syndrome cell adhesion molecule (DSCAM) mRNA in the vertebrate central nervous system. Journal of Neurogenetics, 31(4), 223-230.

Jia, Y. L., Fu, Z. X., Zhang, B. H., & Jia, Y. J. (2017). Hippocampal overexpression of Down syndrome cell adhesion molecule in amyloid precursor protein transgenic mice. Brazilian Journal of Medical and Biological Research, 50.

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For research use only. Not intended for any clinical use.

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