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Mouse Anti-DNMT3A Recombinant Antibody (15B82) (CBMAB-D1415-YC)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
15B82
Antibody Isotype
IgG
Application
ICC, IF, IHC-Fr, IP, WB

Basic Information

Immunogen
Recombinant protein corresponding to mouse Dnmt3a, expressed in bacteria
Specificity
Human, Mouse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DNA Methyltransferase 3 Alpha
Introduction
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. DNMT3A is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. DNMT3A localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
Entrez Gene ID
Human1788
Mouse13435
UniProt ID
HumanQ9Y6K1
MouseO88508
Alternative Names
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. DNMT3A is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. DNMT3A localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
Function
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development (PubMed:12138111, PubMed:16357870, PubMed:30478443).

DNA methylation is coordinated with methylation of histones (PubMed:12138111, PubMed:16357870, PubMed:30478443).

It modifies DNA in a non-processive manner and also methylates non-CpG sites (PubMed:12138111, PubMed:16357870, PubMed:30478443).

May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1 (By similarity).

Plays a role in paternal and maternal imprinting (By similarity).

Required for methylation of most imprinted loci in germ cells (By similarity).

Acts as a transcriptional corepressor for ZBTB18 (By similarity).

Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites (By similarity).

Can actively repress transcription through the recruitment of HDAC activity (By similarity).

Also has weak auto-methylation activity on Cys-710 in absence of DNA (By similarity).
Biological Process
Aging Source: Ensembl
Cellular response to amino acid stimulus Source: Ensembl
Cellular response to ethanol Source: Ensembl
Cellular response to hypoxia Source: Ensembl
DNA methylation Source: UniProtKB
DNA methylation-dependent heterochromatin assembly Source: Ensembl
DNA methylation involved in embryo development Source: Ensembl
DNA methylation involved in gamete generation Source: Ensembl
Hepatocyte apoptotic process Source: Ensembl
Mitotic cell cycle Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Neuron differentiation Source: Ensembl
Positive regulation of cell death Source: Ensembl
Regulation of gene expression by genetic imprinting Source: UniProtKB
Response to cocaine Source: Ensembl
Response to drug Source: Ensembl
Response to estradiol Source: Ensembl
Response to ionizing radiation Source: Ensembl
Response to lead ion Source: Ensembl
Response to toxic substance Source: Ensembl
Response to vitamin A Source: Ensembl
Spermatogenesis Source: Ensembl
Cellular Location
Nucleus; Cytoplasm; Chromosome. Accumulates in the major satellite repeats at pericentric heterochromatin.
Involvement in disease
Tatton-Brown-Rahman syndrome (TBRS):
An overgrowth syndrome characterized by a distinctive facial appearance, tall stature and intellectual disability. Facial gestalt is characterized by a round face, heavy horizontal eyebrows and narrow palpebral fissures. Less common features include atrial septal defects, seizures, umbilical hernia, and scoliosis.
Leukemia, acute myelogenous (AML):
A subtype of acute leukemia, a cancer of the white blood cells. AML is a malignant disease of bone marrow characterized by maturational arrest of hematopoietic precursors at an early stage of development. Clonal expansion of myeloid blasts occurs in bone marrow, blood, and other tissue. Myelogenous leukemias develop from changes in cells that normally produce neutrophils, basophils, eosinophils and monocytes.
Heyn-Sproul-Jackson syndrome (HESJAS):
An autosomal dominant form of microcephalic dwarfism. Affected individuals have intrauterine growth retardation, postnatal growth restrictions, proportionate short stature, microcephaly, severe developmental delay and impaired intellectual development. More variable features include sparse hair, short broad metacarpals and phalanges, and mild recurrent infections.
PTM
Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
Auto-methylated at Cys-710: auto-methylation takes place in absence of DNA substrate and inactivates the DNA methyltransferase activity. Inactivation by auto-methylation may be used to inactivate unused DNA methyltransferases in the cell.
More Infomation

Loughland, I., Little, A., & Seebacher, F. (2021). DNA methyltransferase 3a mediates developmental thermal plasticity. BMC biology, 19(1), 1-11.

Small, L., Ingerslev, L. R., Manitta, E., Laker, R. C., Hansen, A. N., Deeney, B., ... & Barrès, R. (2021). Ablation of DNA-methyltransferase 3A in skeletal muscle does not affect energy metabolism or exercise capacity. PLoS genetics, 17(1), e1009325.

Wei, J., Cheng, J., Waddell, N. J., Wang, Z. J., Pang, X., Cao, Q., ... & Yan, Z. (2021). DNA methyltransferase 3A is involved in the sustained effects of chronic stress on synaptic functions and behaviors. Cerebral Cortex, 31(4), 1998-2012.

Wei, D., Yu, G., & Zhao, Y. (2019). MicroRNA-30a-3p inhibits the progression of lung cancer via the PI3K/AKT by targeting DNA methyltransferase 3a. OncoTargets and therapy, 12, 7015.

Zhang, Z. M., Lu, R., Wang, P., Yu, Y., Chen, D., Gao, L., ... & Song, J. (2018). Structural basis for DNMT3A-mediated de novo DNA methylation. Nature, 554(7692), 387-391.

Cui, H., Hu, Y., Guo, D., Zhang, A., Gu, Y., Zhang, S., ... & Fan, H. (2018). DNA methyltransferase 3A isoform b contributes to repressing E-cadherin through cooperation of DNA methylation and H3K27/H3K9 methylation in EMT-related metastasis of gastric cancer. Oncogene, 37(32), 4358-4371.

Wang, L., Yao, J., Sun, H., He, K., Tong, D., Song, T., & Huang, C. (2017). MicroRNA‑101 suppresses progression of lung cancer through the PTEN/AKT signaling pathway by targeting DNA methyltransferase 3A. Oncology letters, 13(1), 329-338.

Cole, C. B., Russler-Germain, D. A., Ketkar, S., Verdoni, A. M., Smith, A. M., Bangert, C. V., ... & Ley, T. J. (2017). Haploinsufficiency for DNA methyltransferase 3A predisposes hematopoietic cells to myeloid malignancies. The Journal of clinical investigation, 127(10), 3657-3674.

Shao, Z., Xu, P., Xu, W., Li, L., Liu, S., Zhang, R., ... & Luo, C. (2017). Discovery of novel DNA methyltransferase 3A inhibitors via structure-based virtual screening and biological assays. Bioorganic & Medicinal Chemistry Letters, 27(2), 342-346.

Jia, Y., Li, T., Huang, X., Xu, X., Zhou, X., Jia, L., ... & Duan, T. (2017). Dysregulated DNA methyltransferase 3A upregulates IGFBP5 to suppress trophoblast cell migration and invasion in preeclampsia. Hypertension, 69(2), 356-366.

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For research use only. Not intended for any clinical use.

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