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Mouse Anti-DCTD Recombinant Antibody (CBYCD-185) (V2LY-0125-LY1236)


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Published Data
V2LY-0125-LY1236-1
Mouse Anti-DCTD Recombinant Antibody (CBYCD-185) (V2LY-0125-LY1236) in WB
Comparison of the amount of CDD (a) and dCMP deaminase (b) in various cell lines. The data are normalised to the amount of CDD measured in HepG2 cells (a) and to the amount of dCMP deaminase in MRC-5 cells (b), both equal to 100%. The data are presented as the mean±SD. Below both graphs are examples of the western blots corresponding to the specific cells displayed in the graph. ...View More
Ligasová, A., Piskláková, B., Friedecký, D., & Koberna, K. (2023). A new technique for the analysis of metabolic pathways of cytidine analogues and cytidine deaminase activities in cells. Scientific Reports, 13(1), 20530. Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYCD-185
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Full length DCTD of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/mL
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
DCMP Deaminase
Entrez Gene ID
1635
UniProt ID
P32321
Function
Supplies the nucleotide substrate for thymidylate synthetase.
Biological Process
dTMP biosynthetic process Source: GO_Central
dUMP biosynthetic process Source: GO_Central
Nucleobase-containing small molecule interconversion Source: Reactome
Pyrimidine nucleotide metabolic process Source: ProtInc
Cellular Location
Cytosol
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For research use only. Not intended for any clinical use.

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