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Mouse Anti-DCC Recombinant Antibody (CBYCD-176) (V2LY-0125-LY1172)


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Published Data
V2LY-0125-LY1172-1
Mouse Anti-DCC Recombinant Antibody (CBYCD-176) (V2LY-0125-LY1172) in IF
Representative images of TH + dopamine neurons and fibers staining by immunofluorescence, in the SN and the striatum (Str) 6 weeks after CTL (AAV6-GFP); Cre (AAV6-GFP Cre); sh DCC (AAV6-GFP Cre + sh DCC); and shUNC5B (AAV6 GFP Cre + Sh-UNC5B) injection into the SN of Netrin-1fl/fl mice. (Scale bar, 2,000 µm). ...View More
Jasmin, M., Ahn, E. H., Voutilainen, M. H., Fombonne, J., Guix, C., Viljakainen, T., ... & Ye, K. (2021). Netrin-1 and its receptor DCC modulate survival and death of dopamine neurons and Parkinson's disease features. The EMBO journal, 40(3), e105537. Distributed under Open Access license CC BY 3.0, without modification.
V2LY-0125-LY1172-2
Mouse Anti-DCC Recombinant Antibody (CBYCD-176) (V2LY-0125-LY1172) in Immunoblotting
Immunoblot of netrin-1, DCC, UNC5B, active caspase-3, alpha-synuclein FL, alpha-synuclein pS129, and tyrosine hydroxylase (TH) levels in age-matched controls (n = 3) vs Parkinson's disease patients brain samples (n = 3) (left panel). Band quantification bar graph (right panel). Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one-way ANOVA followed by post hoc Tukey test for multiple group comparison. **P < 0.01. N = 3 replicates. ...View More
Jasmin, M., Ahn, E. H., Voutilainen, M. H., Fombonne, J., Guix, C., Viljakainen, T., ... & Ye, K. (2021). Netrin-1 and its receptor DCC modulate survival and death of dopamine neurons and Parkinson's disease features. The EMBO journal, 40(3), e105537. Distributed under Open Access license CC BY 3.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYCD-176
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1159-1363 mapping near the C-terminus of human DCC.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
ELISA1:100-1:1,000
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/mL
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
DCC Netrin 1 Receptor
Entrez Gene ID
Human1630
Mouse13176
Rat25311
UniProt ID
HumanP43146
MouseP70211
RatQ63155
Function
Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene.
Biological Process
Anterior/posterior axon guidance Source: Ensembl
Apoptotic process Source: ProtInc
Axon guidance Source: Reactome
Axonogenesis Source: ProtInc
Dorsal/ventral axon guidance Source: Ensembl
Extrinsic apoptotic signaling pathway in absence of ligand Source: Reactome
Negative regulation of collateral sprouting Source: BHF-UCL
Negative regulation of dendrite development Source: BHF-UCL
Negative regulation of netrin-activated signaling pathway Source: Reactome
Negative regulation of neuron projection development Source: BHF-UCL
Neuron migration Source: Ensembl
Postsynaptic modulation of chemical synaptic transmission Source: Ensembl
Regulation of neuron death Source: UniProtKB
Spinal cord ventral commissure morphogenesis Source: Ensembl
Cellular Location
Membrane
Involvement in disease
Mirror movements 1 (MRMV1):
A disorder characterized by contralateral involuntary movements that mirror voluntary ones. While mirror movements are occasionally found in young children, persistence beyond the age of 10 is abnormal. Mirror movements occur more commonly in the upper extremities. Some MRMV1 patients have agenesis of the corpus callosum.
Gaze palsy, familial horizontal, with progressive scoliosis, 2, with impaired intellectual development (HGPPS2):
An autosomal recessive neurologic disorder characterized by global developmental delay, delayed walking, intellectual disability, horizontal gaze palsy, and childhood-onset progressive scoliosis.
Topology
Extracellular: 26-1097
Helical: 1098-1122
Cytoplasmic: 1123-1447
PTM
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
More Infomation

Chen, G., Kang, S. S., Wang, Z., Ahn, E. H., Xia, Y., Liu, X., ... & Ye, K. (2021). Netrin-1 receptor UNC5C cleavage by active δ-secretase enhances neurodegeneration, promoting Alzheimer’s disease pathologies. Science advances, 7(16), eabe4499.

Li, H. J., Qu, N., Hui, L., Cai, X., Zhang, C. Y., Zhong, B. L., ... & Li, Y. (2020). Further confirmation of netrin 1 receptor (DCC) as a depression risk gene via integrations of multi-omics data. Translational psychiatry, 10(1), 1-15.

Vosberg, D. E., Leyton, M., & Flores, C. (2020). The Netrin-1/DCC guidance system: dopamine pathway maturation and psychiatric disorders emerging in adolescence. Molecular psychiatry, 25(2), 297-307.

Torres-Berrío, A., Hernandez, G., Nestler, E. J., & Flores, C. (2020). The Netrin-1/DCC guidance cue pathway as a molecular target in depression: translational evidence. Biological Psychiatry, 88(8), 611-624.

Kang, D. S., Yang, Y. R., Lee, C., Park, B., Park, K. I., Seo, J. K., ... & Suh, P. G. (2018). Netrin‐1/DCC‐mediated PLC γ1 activation is required for axon guidance and brain structure development. EMBO reports, 19(11), e46250.

Liu, Y., Bhowmick, T., Liu, Y., Gao, X., Mertens, H. D., Svergun, D. I., ... & Meijers, R. (2018). Structural basis for Draxin-Modulated Axon guidance and fasciculation by Netrin-1 through DCC. Neuron, 97(6), 1261-1267.

Torres-Vega, E., Mancheño, N., Cebrián-Silla, A., Herranz-Pérez, V., Chumillas, M. J., Moris, G., ... & Bataller, L. (2017). Netrin-1 receptor antibodies in thymoma-associated neuromyotonia with myasthenia gravis. Neurology, 88(13), 1235-1242.

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For research use only. Not intended for any clinical use.

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