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Mouse Anti-DBH Recombinant Antibody (2Q44) (CBMAB-D0288-YC)


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Summary

Host Animal
Mouse
Specificity
Cattle
Clone
2Q44
Antibody Isotype
IgG1
Application
IF

Basic Information

Immunogen
Purified bovine DBH
Specificity
Cattle
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Dopamine Beta-Hydroxylase
Introduction
DBH is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. The protein, expressed in neuroscretory vesicles and chromaffin granules of the adrenal medulla, catalyzes the conversion of dopamine to norepinephrine, which functions as both a hormone and as the main neurotransmitter of the sympathetic nervous system. The enzyme encoded by this gene exists exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. Mutations in this gene cause dopamine beta-hydroxylate deficiency in human patients, characterized by deficits in autonomic and cardiovascular function, including hypotension and ptosis. Polymorphisms in this gene may play a role in a variety of psychiatric disorders.
Entrez Gene ID
280758
UniProt ID
P15101
Alternative Names
Dopamine Beta-Hydroxylase; Dopamine Beta-Hydroxylase (Dopamine Beta-Monooxygenase); Dopamine Beta-Monooxygenase; EC 1.14.17.1; DBM;
Function
Conversion of dopamine to noradrenaline.
Biological Process
Behavioral response to ethanol Source: Ensembl
Blood vessel remodeling Source: Ensembl
Catecholamine biosynthetic process Source: Reactome
Chemical synaptic transmission Source: ProtInc
Dopamine catabolic process Source: UniProtKB
Fear response Source: Ensembl
Glucose homeostasis Source: Ensembl
Homoiothermy Source: Ensembl
Leukocyte mediated immunity Source: Ensembl
Leukocyte migration Source: Ensembl
Locomotory behavior Source: Ensembl
Maternal behavior Source: Ensembl
Memory Source: Ensembl
Norepinephrine biosynthetic process Source: UniProtKB
Octopamine biosynthetic process Source: GO_Central
Positive regulation of cold-induced thermogenesis Source: YuBioLab
Positive regulation of vasoconstriction Source: Ensembl
Regulation of cell population proliferation Source: Ensembl
Regulation of extrinsic apoptotic signaling pathway Source: Ensembl
Response to amphetamine Source: Ensembl
Response to pain Source: Ensembl
Visual learning Source: Ensembl
Cellular Location
Soluble dopamine beta-hydroxylase: Secreted; Secretory vesicle lumen; Chromaffin granule lumen; Secretory vesicle membrane; Chromaffin granule membrane
Involvement in disease
Orthostatic hypotension 1 (ORTHYP1):
A form of orthostatic hypotension due to congenital dopamine beta-hydroxylase deficiency. Orthostatic hypotension, also known as postural hypotension, is a finding defined as a 20-mm Hg decrease in systolic pressure or a 10-mm Hg decrease in diastolic pressure occurring 3 minutes after a person has risen from supine to standing. Symptoms include dizziness, blurred vision, and sometimes syncope. ORTHYP1 is an autosomal recessive condition apparent from infancy or early childhood and characterized by low plasma and urinary levels of norepinephrine and epinephrine, and episodic hypoglycemia.
Topology
Cytoplasmic: 1-176
Helical: 17-37
Intragranular: 38-617
PTM
N-glycosylated.
Proteolytic cleavage after the membrane-anchor leads to the release of the soluble form.
More Infomation

Hobgood, D. K. (2021). ABO B gene is associated with introversion personality tendancies through linkage with dopamine beta hydroxylase gene. Medical Hypotheses, 148, 110513.

Polasik, D., Konieczna, A., Terman, A., & Dybus, A. (2021). The association of C789A polymorphism in the dopamine beta-hydroxylase gene (DBH) and aggressive behaviour in dogs. Acta Veterinaria Brno, 90(3), 295-299.

Wassenberg, T., Deinum, J., van Ittersum, F. J., Kamsteeg, E. J., Pennings, M., Verbeek, M. M., ... & Willemsen, M. A. (2021). Clinical presentation and long‐term follow‐up of dopamine beta hydroxylase deficiency. Journal of inherited metabolic disease, 44(3), 554-565.

Kundu, S., Saini, M., Dey, S. K., & Kundu, S. (2020). Dopamine Beta Hydroxylase: An Enzyme with Therapeutic Potential to Combat Neural and Cardiovascular Diseases. In Frontiers in Protein Structure, Function, and Dynamics (pp. 339-357). Springer, Singapore.

Barbanti, P., Guadagni, F., De Marchis, M. L., Ialongo, C., Egeo, G., Fofi, L., ... & Palmirotta, R. (2019). Dopamine-beta-hydroxylase 19-bp insertion/deletion polymorphism affects medication overuse in patients with chronic migraine. Neurological Sciences, 40(8), 1717-1724.

Shalev, N., Vangkilde, S., Neville, M. J., Tunbridge, E. M., Nobre, A. C., & Chechlacz, M. (2019). Dissociable catecholaminergic modulation of visual attention: differential effects of catechol-O-methyltransferase and dopamine beta-hydroxylase genes on visual attention. Neuroscience, 412, 175-189.

Tang, S., Yao, B., Li, N., Lin, S., & Huang, Z. (2018). Association of dopamine beta-hydroxylase polymorphisms with alzheimer’s disease, Parkinson’s disease and schizophrenia: evidence based on currently available loci. Cellular Physiology and Biochemistry, 51(1), 411-428.

Yamaguchi, H., Hopf, F. W., Li, S. B., & de Lecea, L. (2018). In vivo cell type-specific CRISPR knockdown of dopamine beta hydroxylase reduces locus coeruleus evoked wakefulness. Nature communications, 9(1), 1-8.

Cheng, W., Ka, Y. W., & Chang, C. C. (2017). Involvement of dopamine beta-hydroxylase in the neuroendocrine-immune regulatory network of white shrimp, Litopenaeus vannamei. Fish & Shellfish Immunology, 68, 92-101.

Bozek, T., Blazekovic, A., Perkovic, M. N., Jercic, K. G., Sustar, A., Smircic-Duvnjak, L., ... & Borovecki, F. (2017). The influence of dopamine-beta-hydroxylase and catechol O-methyltransferase gene polymorphism on the efficacy of insulin detemir therapy in patients with type 2 diabetes mellitus. Diabetology & metabolic syndrome, 9(1), 1-11.

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For research use only. Not intended for any clinical use.

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