Mouse Anti-DAD1 Recombinant Antibody (2B4-C8) (CBMAB-D0173-YC)

Name: Mouse Anti-DAD1 Recombinant Antibody (2B4-C8)
SKU: CBMAB-D0173-YC
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

2B4-C8

Antibody Isotype

IgG2b, κ

Application

ELISA

Basic Information

Immunogen

Full-length recombinant DAD1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Target

Full Name

Defender Against Cell Death 1

Entrez Gene ID

1603

UniProt ID

P61803

Function

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc3Man9GlcNAc2 in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:22467853).

N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity).

Required for the assembly of both SST3A- and SS3B-containing OST complexes. Loss of the DAD1 protein triggers apoptosis (PubMed:22467853).

Biological Process

Apoptotic process Source: UniProtKB-KW
Blastocyst development Source: Ensembl
Negative regulation of apoptotic process Source: ProtInc
Protein glycosylation Source: UniProtKB
Protein N-linked glycosylation Source: ARUK-UCL
Protein N-linked glycosylation via asparagine Source: Reactome
Regulation of protein stability Source: ARUK-UCL
Response to drug Source: Ensembl
Response to nutrient Source: Ensembl
Viral protein processing Source: Reactome

Cellular Location

Endoplasmic reticulum membrane

Topology

Cytoplasmic: 2-30
Helical: 31-51
Lumenal: 52
Helical: 53-73
Cytoplasmic: 74-92
Helical: 93-113

More Infomation

References

Bao, Y., Mei, Y. Q., Xu, X., Liu, Y., & Wu, Z. Y. (2021). Changes in the promoter of a defender against apoptotic cell death gene affect its expression in upland cotton. Journal of Systematics and Evolution, 59(5), 1018-1026.

Luo, Y., Wu, Y., Huang, H., Yi, N., & Chen, Y. (2021). Emerging role of BAD and DAD1 as potential targets and biomarkers in cancer. Oncology Letters, 22(6), 1-13.

Yan, Q., Si, J., Cui, X., Peng, H., Jing, M., Chen, X., ... & Dou, D. (2019). GmDAD1, a conserved defender against cell death 1 (DAD1) from soybean, positively regulates plant resistance against phytophthora pathogens. Frontiers in plant science, 10, 107.

Wang, M. Q., Wang, B. J., Liu, M., Jiang, K. Y., & Wang, L. (2018). Molecular characterization of a defender against apoptotic cell death 1 gene (CfDAD1) from the mollusk Chlamys farreri. Invertebrate Survival Journal, 15(1), 294-301.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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