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Mouse Anti-CYTH2 Recombinant Antibody (CBXC-2916) (V2LY-0125-LY1029)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Horse, Dog, Cattle, Pig
Clone
CBXC-2916
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 31-67 mapping near the N-terminus of human cytohesin-2.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Horse, Dog, Cattle, Pig
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
ELISA1:100-1:1,000
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/mL
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
cytohesin 2
Entrez Gene ID
Human9266
Rat116692
UniProt ID
HumanQ99418
RatP63035
Function
Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Activates ARF factors through replacement of GDP with GTP (By similarity).

The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane (PubMed:17398095).

Involved in neurite growth (By similarity).
Biological Process
Actin cytoskeleton organization Source: ProtInc
Endocytosis Source: ProtInc
Regulation of ARF protein signal transduction Source: InterPro
Cellular Location
Cytoplasm; Cell membrane; Cell projection; Growth cone; Tight junction; Adherens junction. Both isoform 1 and isoform 2 are recruited to the cell membrane through its association with ARL4A, ARL4C and ARL4D. They require also interaction with phosphoinositides for targeting to plasma membrane (PubMed:17398095). In differentiating neuroblastoma cells, colocalizes with CCDC120 in both neurite shaft and growth cone areas.
More Infomation

Yi, C., Cai, C., Cheng, Z., Zhao, Y., Yang, X., Wu, Y., ... & Zhang, A. (2022). Genome-wide CRISPR-Cas9 screening identifies the CYTH2 host gene as a potential therapeutic target of influenza viral infection. Cell Reports, 38(13), 110559.

Miyamoto, Y., Torii, T., Homma, K., Oizumi, H., Ohbuchi, K., Mizoguchi, K., ... & Yamauchi, J. (2022). The adaptor SH2B1 and the phosphatase PTP4A1 regulate the phosphorylation of cytohesin-2 in myelinating Schwann cells in mice. Science Signaling, 15(718), eabi5276.

Wang, Y., Cil, C., Harnett, M. M., & Pineda, M. (2021). GEF Cytohesin-2/ARNO: a novel bridge between cell migration and immunoregulation in synovial fibroblasts. bioRxiv.

Ito, H., Morishita, R., Noda, M., Ishiguro, T., Nishikawa, M., & Nagata, K. I. (2021). The synaptic scaffolding protein CNKSR2 interacts with CYTH2 to mediate hippocampal granule cell development. Journal of Biological Chemistry, 297(6).

Heun, Y., Gräff, P., Lagara, A., Schelhorn, R., Mettler, R., Pohl, U., & Mannell, H. (2020). The GEF Cytohesin-2/ARNO mediates resistin induced phenotypic switching in vascular smooth muscle cells. Scientific reports, 10(1), 1-10.

Namislo, A. M. (2019). The differential regulation of podosome formation by Cytohesin-2 is mediated via α5β1 integrin and the small GTPase RhoA.

Ito, A., Fukaya, M., Saegusa, S., Kobayashi, E., Sugawara, T., Hara, Y., ... & Sakagami, H. (2018). Pallidin is a novel interacting protein for cytohesin‐2 and regulates the early endosomal pathway and dendritic formation in neurons. Journal of Neurochemistry, 147(2), 153-177.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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