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Mouse Anti-CYP7B1 Recombinant Antibody (7E5) (CBMAB-C8673-LY)


See all CYP7B1 antibodies
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Summary

Host Animal
Mouse
Specificity
Human
Clone
7E5
Antibody Isotype
IgG2b
Application
WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
1% BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cytochrome P450 Family 7 Subfamily B Member 1
Introduction
CYP7B1 (Cytochrome P450 Family 7 Subfamily B Member 1) is a Protein Coding gene. Diseases associated with CYP7B1 include Spastic Paraplegia 5A, Autosomal Recessive and Bile Acid Synthesis Defect, Congenital, 3. Among its related pathways are Metabolism and Cytochrome P450 - arranged by substrate type. Gene Ontology (GO) annotations related to this gene include iron ion binding and oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen.
An important paralog of this gene is CYP7A1.
Entrez Gene ID
9420
UniProt ID
O75881
Alternative Names
Cytochrome P450 Family 7 Subfamily B Member 1; Cytochrome P450, Subfamily VIIB (Oxysterol 7 Alpha-Hydroxylase), Polypeptide 1; Oxysterol 7-Alpha-Hydroxylase; Cytochrome P450 7B1; Cytochrome P450, Family 7, Subfamily B, Polypeptide 1; Spastic Paraplegia 5A (Autosomal Recessive);
Function
A cytochrome P450 monooxygenase involved in the metabolism of endogenous oxysterols and steroid hormones, including neurosteroids (PubMed:10588945, PubMed:24491228).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:10588945, PubMed:24491228).

Catalyzes the hydroxylation of carbon hydrogen bonds of steroids with a preference for 7-alpha position (PubMed:10588945, PubMed:24491228).

Usually metabolizes steroids carrying a hydroxy group at position 3, functioning as a 3-hydroxy steroid 7-alpha hydroxylase (PubMed:24491228).

Hydroxylates oxysterols, including 25-hydroxycholesterol and (25R)-cholest-5-ene-3beta,26-diol toward 7-alpha hydroxy derivatives, which may be transported to the liver and converted to bile acids (PubMed:9802883, PubMed:10588945).

Via its product 7-alpha,25-dihydroxycholesterol, a ligand for the chemotactic G protein-coupled receptor GPR183/EBI2, regulates B cell migration in germinal centers of lymphoid organs, thus guiding efficient maturation of plasma B cells and overall antigen-specific humoral immune response (By similarity).

7-alpha hydroxylates neurosteroids, including 3beta-hydroxyandrost-5-en-17-one (dehydroepiandrosterone) and pregnenolone, both involved in hippocampus-associated memory and learning (PubMed:24491228).

Metabolizes androstanoids toward 6- or 7-alpha hydroxy derivatives (PubMed:24491228).
Biological Process
B cell chemotaxis Source: UniProtKB
Bile acid biosynthetic process Source: GO_Central
Cholesterol homeostasis Source: GO_Central
Cholesterol metabolic process Source: UniProtKB-KW
Negative regulation of intracellular estrogen receptor signaling pathway Source: Ensembl
Positive regulation of epithelial cell proliferation Source: Ensembl
Prostate gland epithelium morphogenesis Source: Ensembl
Sterol metabolic process Source: Reactome
Cellular Location
Endoplasmic reticulum membrane; Microsome membrane
Involvement in disease
Spastic paraplegia 5A, autosomal recessive (SPG5A):
A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
Congenital bile acid synthesis defect 3 (CBAS3):
A disorder resulting in severe cholestasis, cirrhosis and liver synthetic failure. Hepatic microsomal oxysterol 7-alpha-hydroxylase activity is undetectable.
More Infomation

Evangelakos, I., Schwinge, D., Worthmann, A., John, C., Roeder, N., Pertzborn, P., ... & Heeren, J. (2021). Oxysterol 7-α Hydroxylase (CYP7B1) Attenuates Metabolic-Associated Fatty Liver Disease in Mice at Thermoneutrality. Cells, 10(10), 2656.

Li, X., Zhang, L., Shi, X., Liao, T., Zhang, N., Gao, Y., ... & Wang, P. (2021). MicroRNA-10a-3p Improves Cartilage Degeneration by Regulating CH25H-CYP7B1-RORα Mediated Cholesterol Metabolism in Knee Osteoarthritis Rats. Frontiers in pharmacology, 12, 1407.

Le Cornet, C., Walter, B., Sookthai, D., Johnson, T. S., Kühn, T., Herpel, E., ... & Fortner, R. T. (2020). Circulating 27-hydroxycholesterol and breast cancer tissue expression of CYP27A1, CYP7B1, LXR-β, and ERβ: results from the EPIC-Heidelberg cohort. Breast Cancer Research, 22(1), 1-10.

Kakiyama, G., Marques, D., Martin, R., Takei, H., Rodriguez-Agudo, D., LaSalle, S. A., ... & Pandak, W. M. (2020). Insulin resistance dysregulates CYP7B1 leading to oxysterol accumulation: A pathway for NAFL to NASH transition. Journal of lipid research, 61(12), 1629-1644.

Chen, J. Y., Wu, J. F., Kimura, A., Nittono, H., Liou, B. Y., Lee, C. S., ... & Chen, H. L. (2020). AKR1D1 and CYP7B1 mutations in patients with inborn errors of bile acid metabolism: Possibly underdiagnosed diseases. Pediatrics & Neonatology, 61(1), 75-83.

Choi, W. S., Lee, G., Song, W. H., Koh, J. T., Yang, J., Kwak, J. S., ... & Chun, J. S. (2019). The CH25H–CYP7B1–RORα axis of cholesterol metabolism regulates osteoarthritis. Nature, 566(7743), 254-258.

Shi, S. Z., Lee, E. J., Lin, Y. J., Chen, L., Zheng, H. Y., He, X. Q., ... & Xin, H. B. (2019). Recruitment of monocytes and epigenetic silencing of intratumoral CYP7B1 primarily contribute to the accumulation of 27-hydroxycholesterol in breast cancer. American journal of cancer research, 9(10), 2194.

Kakiyama, G., Marques, D., Takei, H., Nittono, H., Erickson, S., Fuchs, M., ... & Pandak, W. M. (2019). Mitochondrial oxysterol biosynthetic pathway gives evidence for CYP7B1 as controller of regulatory oxysterols. The Journal of steroid biochemistry and molecular biology, 189, 36-47.

Meljon, A., Crick, P. J., Yutuc, E., Yau, J. L., Seckl, J. R., Theofilopoulos, S., ... & Griffiths, W. J. (2019). Mining for oxysterols in Cyp7b1−/− mouse brain and plasma: relevance to spastic paraplegia type 5. Biomolecules, 9(4), 149.

Kim, H. J., Sharma, A., Lee, S. H., Lee, D. H., Cho, Y. M., Yang, B. S., & Lee, S. H. (2017). Genetic association of PLAG1, SCD, CYP7B1 and FASN SNPs and their effects on carcass weight, intramuscular fat and fatty acid composition in Hanwoo steers (Korean cattle). Animal genetics, 48(2), 251-252.

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For research use only. Not intended for any clinical use.

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