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Mouse Anti-CTCFL Recombinant Antibody (C4674) (CBMAB-C4674-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
C4674
Antibody Isotype
IgG3, κ
Application
WB, IF, IHC-P, ELISA, IP

Basic Information

Immunogen
Synthetic peptide within the BORIS C-terminal domain (aa 614-648).
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG3, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:2,000
IHC-P1:10-1:500
IF(ICC)1:10-1:500
ELISA1:100-1:2,000
IP1:10-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.02% sodium azide
Concentration
1 mg/ml

Target

Full Name
CCCTC-Binding Factor Like
Entrez Gene ID
140690
UniProt ID
Q8NI51
Function
Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites (By similarity).

Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.
Biological Process
Cell cycle Source: UniProtKB-KW
DNA methylation involved in gamete generation Source: UniProtKB
Histone methylation Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of gene expression by genetic imprinting Source: UniProtKB
Regulation of histone H3-K4 methylation Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleus; Cytoplasm
More Infomation

Rao, G. K., Makani, V. K. K., Mendonza, J. J., Edathara, P. M., Patel, N., Ramakrishna, M., ... & Bhadra, M. P. (2021). Downregulation of BORIS/CTCFL leads to ROS‐dependent cellular senescence and drug sensitivity in MYCN‐amplified neuroblastoma. The FEBS journal.

Moscona, R., Janssen, S., Elchebly, M., Papadakis, A., Spatz, A., & Rubin, E. (2022). ATAC-Seq and RNA-Seq combined analysis elucidates the impact of BORIS/CTCFL on melanoma progression, mediated by chromatin accessibility and gene expression alterations. bioRxiv.

Chen, M. M., Zhao, R. C., Chen, K. F., Huang, Y., Liu, Z. J., Wei, Y. G., ... & Qin, Y. (2020). Hypomethylation of CTCFL promoters as a noninvasive biomarker in plasma from patients with hepatocellular carcinoma. Neoplasma, 67(04), 909-15.

Janssen, S. M., Moscona, R., Elchebly, M., Papadakis, A. I., Redpath, M., Wang, H., ... & Spatz, A. (2020). BORIS/CTCFL promotes a switch from a proliferative towards an invasive phenotype in melanoma cells. Cell death discovery, 6(1), 1-17.

Debaugny, R. E., & Skok, J. A. (2020). CTCF and CTCFL in cancer. Current opinion in genetics & development, 61, 44-52.

Gong, M., Yan, C., Jiang, Y., Meng, H., Feng, M., & Cheng, W. (2019). Genome‑wide bioinformatics analysis reveals CTCFL is upregulated in high‑grade epithelial ovarian cancer. Oncology letters, 18(4), 4030-4039.

Voutsadakis, I. A. (2018). Molecular Lesions of Insulator CTCF and Its Paralogue CTCFL (BORIS) in cancer: an analysis from published genomic studies. High-throughput, 7(4), 30.

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For research use only. Not intended for any clinical use.

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