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Mouse Anti-CSPG4 Recombinant Antibody (CBCNC-088) (CBMAB-C0982-CN)


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Summary

Host Animal
Mouse
Specificity
Cat
Clone
CBCNC-088
Antibody Isotype
IgG1
Application
ICC, IHC, IP, WB

Basic Information

Specificity
Cat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.2
Preservative
No preservatives

Target

Full Name
Chondroitin Sulfate Proteoglycan 4
Introduction
CSPG4P1Y (Chondroitin Sulfate Proteoglycan 4 Pseudogene 1, Y-Linked) is a Pseudogene.
Entrez Gene ID
101097242
UniProt ID
M3WGW7
Alternative Names
Chondroitin Sulfate Proteoglycan 4; Melanoma-Associated Chondroitin Sulfate Proteoglycan; Chondroitin Sulfate Proteoglycan 4 (Melanoma-Associated); Melanoma Chondroitin Sulfate Proteoglycan; Chondroitin Sulfate Proteoglycan NG2; MCSP; MEL-CSPG;
Function
Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
Biological Process
Activation of MAPK activity Source: Ensembl
Angiogenesis Source: UniProtKB-KW
Cell population proliferation Source: Ensembl
Chondroitin sulfate biosynthetic process Source: Reactome
Chondroitin sulfate catabolic process Source: Reactome
Dermatan sulfate biosynthetic process Source: Reactome
Glial cell migration Source: Ensembl
Intracellular signal transduction Source: UniProtKB
Platelet-derived growth factor receptor signaling pathway Source: Ensembl
Positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
Ruffle assembly Source: Ensembl
Substrate-dependent cell migration Source: Ensembl
Tissue remodeling Source: UniProtKB-KW
Cellular Location
Cell membrane; Apical cell membrane; Lamellipodium membrane; Cell surface. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes (By similarity).
Topology
Extracellular: 30-2224
Helical: 2225-2245
Cytoplasmic: 2246-2322
PTM
O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
More Infomation

Uranowska, K., Kalic, T., Valtsanidis, V., Kitzwögerer, M., Breiteneder, H., & Hafner, C. (2021). Expression of chondroitin sulfate proteoglycan 4 (CSPG4) in melanoma cells is downregulated upon inhibition of BRAF. Oncology Reports, 45(4), 1-1.

Uranowska, K., Samadaei, M., Kalic, T., Pinter, M., Breiteneder, H., & Hafner, C. (2021). A chondroitin sulfate proteoglycan 4‑specific monoclonal antibody inhibits melanoma cell invasion in a spheroid model. International journal of oncology, 59(3), 1-12.

Hoffmann, R. M., Crescioli, S., Mele, S., Sachouli, E., Cheung, A., Chui, C. K., ... & Karagiannis, S. N. (2020). A novel antibody-drug conjugate (ADC) delivering a DNA mono-alkylating payload to chondroitin sulfate proteoglycan (CSPG4)-expressing melanoma. Cancers, 12(4), 1029.

Henkel, D., Tatge, H., Schöttelndreier, D., Tao, L., Dong, M., & Gerhard, R. (2020). Receptor binding domains of TcdB from Clostridioides difficile for chondroitin sulfate proteoglycan-4 and frizzled proteins are functionally independent and additive. Toxins, 12(12), 736.

Ferrone, S., & Whiteside, T. L. (2020). Targeting CSPG4 for isolation of melanoma cell-derived exosomes from body fluids. HNO, 68(2), 100-105.

Kasten, B. B., Oliver, P. G., Kim, H., Fan, J., Ferrone, S., Zinn, K. R., & Buchsbaum, D. J. (2018). 212Pb-labeled antibody 225.28 targeted to chondroitin sulfate proteoglycan 4 for triple-negative breast cancer therapy in mouse models. International journal of molecular sciences, 19(4), 925.

Schiffer, D., Mellai, M., Boldorini, R., Bisogno, I., Grifoni, S., Corona, C., ... & Annovazzi, L. (2018). The significance of chondroitin sulfate proteoglycan 4 (CSPG4) in human gliomas. International Journal of Molecular Sciences, 19(9), 2724.

Ilieva, K. M., Cheung, A., Mele, S., Chiaruttini, G., Crescioli, S., Griffin, M., ... & Karagiannis, S. N. (2018). Chondroitin sulfate proteoglycan 4 and its potential as an antibody immunotherapy target across different tumor types. Frontiers in Immunology, 8, 1911.

Hsu, S. H. C., Nadesan, P., Puviindran, V., Stallcup, W. B., Kirsch, D. G., & Alman, B. A. (2018). Effects of chondroitin sulfate proteoglycan 4 (NG2/CSPG4) on soft-tissue sarcoma growth depend on tumor developmental stage. Journal of Biological Chemistry, 293(7), 2466-2475.

Pellegatta, S., Savoldo, B., Di Ianni, N., Corbetta, C., Chen, Y., Patané, M., ... & Dotti, G. (2018). Constitutive and TNFα-inducible expression of chondroitin sulfate proteoglycan 4 in glioblastoma and neurospheres: Implications for CAR-T cell therapy. Science translational medicine, 10(430), eaao2731.

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For research use only. Not intended for any clinical use.

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