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Mouse Anti-CRTC3 Recombinant Antibody (CBFYC-2243) (V2LY-1206-LY1266)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Dog, Pig, Horse, Cattle
Clone
CBFYC-2243
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 563-594 of human TORC3.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Dog, Pig, Horse, Cattle
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
CREB Regulated Transcription Coactivator 3
Entrez Gene ID
Human64784
Mouse70461
Rat365297
UniProt ID
HumanQ6UUV7
MouseQ91X84
RatF1LVL6
Function
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
Biological Process
Energy homeostasis Source: Ensembl
Macrophage activation Source: CACAO
Negative regulation of cAMP-mediated signaling Source: Ensembl
Negative regulation of lipid catabolic process Source: Ensembl
Positive regulation of CREB transcription factor activity Source: GO_Central
Positive regulation of transcription by RNA polymerase II Source: GO_Central
Protein homotetramerization Source: InterPro
Viral process Source: UniProtKB-KW
Cellular Location
Nucleus; Cytoplasm. Appears to be mainly nuclear (PubMed:15454081). Translocates to the nucleus following adenylyl cyclase or MAP kinase activation (PubMed:30611118).
PTM
Phosphorylation/dephosphorylation states of Ser-273 are required for regulating transduction of CREB activity (By similarity). CRTCs/TORCs are inactive when phosphorylated, and active when dephosphorylated at this site (By similarity). May be phosphorylated at Ser-391 by MAPK3/ERK1 and/or MAPK1/ERK2 or by some cyclin-dependent kinases such as CDK1,CDK2 or CDK5 (PubMed:30611118). Following adenylyl cyclase activation, dephosphorylated at Ser-162 and Ser-273 resulting in its dissociation from 14-3-3 proteins probably promoting CRTC3 translocation into the nucleus (PubMed:30611118).
More Infomation

Smith, L. I., Zhao, Z., Walker, J., Lightman, S., & Spiga, F. (2021). Activation and expression of endogenous CREB‐regulated transcription coactivators (CRTC) 1, 2 and 3 in the rat adrenal gland. Journal of Neuroendocrinology, 33(1), e12920.

Liu, J., Wang, L., Chen, W., Li, J., & Shan, T. (2021). CRTC3 regulates the lipid metabolism and adipogenic differentiation of porcine Intramuscular and subcutaneous adipocytes by activating the calcium pathway. Journal of Agricultural and Food Chemistry, 69(25), 7243-7255.

Kim, J. H., Hong, A. R., Kim, Y. H., Yoo, H., Kang, S. W., Chang, S. E., & Song, Y. (2020). JNK suppresses melanogenesis by interfering with CREB-regulated transcription coactivator 3-dependent MITF expression. Theranostics, 10(9), 4017.

Liu, J., Nong, Q., Wang, J., Chen, W., Xu, Z., You, W., ... & Shan, T. (2020). Breed difference and regulatory role of CRTC3 in porcine intramuscular adipocyte. Animal Genetics, 51(4), 521-530.

Xu, Z., Liu, J., You, W., Wang, Y., & Shan, T. (2019). Cold exposure induces nuclear translocation of CRTC3 in brown adipose tissue. Journal of Cellular Biochemistry, 120(6), 9138-9146.

Sonntag, T., Ostojić, J., Vaughan, J. M., Moresco, J. J., Yoon, Y. S., Yates III, J. R., & Montminy, M. (2019). Mitogenic signals stimulate the CREB coactivator CRTC3 through PP2A recruitment. Iscience, 11, 134-145.

Kim, Y. H., Yoo, H., Hong, A. R., Kwon, M., Kang, S. W., Kim, K., & Song, Y. (2018). NEDD4L limits cAMP signaling through ubiquitination of CREB‐regulated transcription coactivator 3. The FASEB Journal, 32(7), 4053-4062.

Yoon, Y. S., Tsai, W. W., Van de Velde, S., Chen, Z., Lee, K. F., Morgan, D. A., ... & Montminy, M. (2018). cAMP-inducible coactivator CRTC3 attenuates brown adipose tissue thermogenesis. Proceedings of the National Academy of Sciences, 115(23), E5289-E5297.

Kim, J. H., Hedrick, S., Tsai, W. W., Wiater, E., Le Lay, J., Kaestner, K. H., ... & Montminy, M. (2017). CREB coactivators CRTC2 and CRTC3 modulate bone marrow hematopoiesis. Proceedings of the National Academy of Sciences, 114(44), 11739-11744.

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For research use only. Not intended for any clinical use.

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